Epigenetic reprogramming associated with specification and early development of primordial germ cells.

• Project/Area Number: 20770186
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SEKI Yoshiyuki The Institute of Physical and Chemical Research, 理工学部, 専任講師 (20435655)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生殖細胞 / エピゲノム / 発生・再生 / 分化全能性

Research Abstract

Birth of life commence with fertilization of sperm and oocyte. Fertilized eggs can from complex and compact body correctly through developmental program. The ability, which one cell can form individual, is called cellular 'totipotency', which is regulated by epigenetic mechanisms. In this research, we focused on epigenetic reprogramming in primordial germ cells to understand how germ cells acquire cellular totipotency. In this result, we identified new gene, Prdm14, and elucidated that Prdm14 deficient mice have infertility in both male and female.

Research Products

• [Journal Article] Specification of the germ cell lineage in mice:A process orchestrated by the PR-domain proteins, Blimpl and Prdm14. (2008)
  • Author(s): Kurimoto, K., Yamaji, M., Seki, Y., Saitou, M.
  • Journal Title: Cell cycle 7(22)
  • Peer Reviewed
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice. (2008)
  • Author(s): Yamaji, M. *, Seki, Y. *, Kurimoto, K. *, Yabuta, Y., Yuasa, M., Shigeta, M., Yamanaka., K., Ohinata., Y., Saitou, M.
  • Journal Title: Nature Genetics 40(8) Pages: 1016-1022
  • Peer Reviewed
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice (2008)
  • Author(s): Yamaji, M., Seki, Y., Kurimoto, M.
  • Journal Title: Nature Genetics 40(8) Pages: 1016-1022
  • Peer Reviewed
• [Journal Article] Specification of the germ cell lineage in mice: A process orchestrated by the PR-domain proteins, Blimp1 and Prdm14 (2008)
  • Author(s): Kurimoto, K.
  • Journal Title: Cell Cycle 7(22) Pages: 3514-3518
  • Peer Reviewed
• [Presentation] 始原生殖細胞において消えるエピゲノムと消えないエピゲノム (2009)
  • Author(s): 関由行
  • Organizer: 第82回生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] 始原生殖細胞において消えるエピゲノムと消えないエピゲノム (2009)
  • Author(s): 関由行
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] マウス始原生殖細胞によるDNA脱メチル化機構の解明 (2009)
  • Author(s): 関由行
  • Organizer: 第3回エピジェネティクス研究会年会
  • Place of Presentation: 学術総合センター、東京
  • Year and Date: 2009-05-22
• [Presentation] 始原生殖細胞によるエピゲノム再編成機構の解明 (2008)
  • Author(s): 関由行
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸
• [Book] Epigenetic reprogramming in primordial germ cell development. Genetical and epigenetical control of mammalian germ cell development and their function. Genetic and epigenetic control of mammalian germ cell development and function. (2008)
  • Author(s): Seki, Y. *, Saitou, M*.
  • Publisher: Research Signpost
• [Remarks]
  • URL: http://sci-tech.ksc.kwansei.ac.jp/~seki/SekiLab..html