Directional control of cell polarization revealed by quantitative measurement and mathematical modeling

• Project/Area Number: 20770187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ARATA Yukinobu The Institute of Physical and Chemical Research, 佐甲細胞情報研究室, 研究員 (40360482)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 画像解析プログラム / 数理モデル / 細胞極性 / 方向制御 / 一分子計測 / タンパク質細胞内動態 / 非対称局在 / PARタンパク質 / 分子生物学 / 細胞生物学 / 発生生物学

Research Abstract

Cells polarize along with the axes of the animal body. Cell polarity is maintained by dynamic equilibrium of protein movements. In this study, I succeeded to apply and develop the new systems to reveal protein dynamics quantitatively to developmental biology (Edge-detection image processing program, Fluorescence correlation spectroscopy (FCS), Total internal reflection fluorescence microscope (TIRFM)). Based on the achievements of this study, I will carry out mathematical modeling to understand cell polarization as a dynamical system.

Research Products

• [Journal Article] Extracellular control of PAR protein localization during asymmetric cell division in the C. elegans embryo (2010)
  • Author(s): Arata Yukinobu, Lee Jen-Yi, Goldstein Bob, Sawa Hitoshi
  • Journal Title: Development
• [Journal Article] Extracellular control of PAR protein localization during asymmetric cell division in the C.elegans embryo. (2010)
  • Author(s): Arata Y, Lee JY, Goldstein B, Sawa H.
  • Journal Title: Development. Volume: 137 Pages: 3337-45
  • Peer Reviewed
• [Presentation] PARタンパク質依存的な細胞極性の方向制御 (2010)
  • Author(s): 荒田幸信
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 宮城県仙台市(東北大学)
  • Year and Date: 2010-09-16
• [Presentation] (2010)
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 宮城
• [Presentation] (2009)
  • Organizer: 第82回日本生化学会大会
• [Presentation] (2009)
  • Organizer: 17th International C. elegans Meeting
• [Presentation] (2009)
  • Organizer: 日本発生生物学会第42回大会
  • Place of Presentation: 新潟
• [Presentation] Directional control of PAR-dependent polarization in C.elegans (2009)
  • Author(s): 荒田幸信
  • Organizer: 発生生物学会年会
  • Place of Presentation: 新潟コンベンションセンター(朱鷺メッセ)
• [Presentation] Directional control of PAR-dependent polarization in C.elegans (2009)
  • Author(s): 荒田幸信
  • Organizer: 17th international C.elegans meeting
  • Place of Presentation: University of California, Los Angeles, California
• [Presentation] 細胞単離・操作法を用いてPARタンパク質依存的な細胞極性を細胞外から方向付けする (2009)
  • Author(s): 荒田幸信
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] PAE-dependent polarization is oriented by an extracellular signal MES-1 in C. elegans (2008)
  • Author(s): 荒田幸信
  • Organizer: C. elegans Development and Evolution meeting
  • Place of Presentation: ウィスコンシン大学
• [Presentation] 線虫の初期胚において、PAR依存的な細胞の極性化はMES-1/SRCシグナルによって方向付けされる (2008)
  • Author(s): 荒田幸信
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] PAE-dependent polarization is oriented by an extracellular signal MES-1 in C. elegans (2008)
  • Author(s): 荒田幸信
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
  • URL: http://www.riken.jp/cell-info/
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/MultiOrg/Multicellular/Publication.html
• [Remarks]
  • URL: http://www.cdb.riken.jp/cfd/HP6/