| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The transcription factors DRE (dehydration responsive element) binding protein 1s (DREB1s)/ C-repeat (CRT) binding factors (CBFs) specifically interact with the DRE/CRT cis-acting element and control the expression of many stress-inducible genes in Arabidopsis thaliana. Rice (Oryza sativa) has 10 genes for DREB1 orthologues, OsDREB1s. We analyzed the 10 OsDREB1 genes comprehensively. Many OsDREB1 genes were induced by cold stress (4℃) specifically, and some OsDREB1 genes were also induced by high-salinity (250mM NaCl) or drought stresses. Although all the OsDREB1s except for OsDREB1D have transactivation activity, there is a difference of DNA binding preference among the 10 OsDREB1s. Overexpression of OsDREB1G which has the highest transactivation activity among the 10 OsDREB1s affected expression of many genes in transgenic rice plants which contain the OsDREB1G controlled under a promoter responsive to an animal hormone, dexamethasone (DEX) and some of the dowmstream genes were also regulated by OsDREB1A. These results indicate that rice has some DREB1 genes and utilizes these genes elaborately according to various stresses.
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