Identification of genes affecting the efficiency of genetic transformation in wheat.

Research Project

Project/Area Number	20780007
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Breeding science
Research Institution	National Agricultural Research Organization
Principal Investigator	ABE Fumitaka National Agricultural Research Organization, 作物研究所麦類遺伝子技術研究チーム, 主任研究員 (30370547)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	植物分子育種 / 形質転換 / 網羅的遺伝子発現解析 / 形質転換効率 / コムギ / 組織培養 / 網羅的発現解析 / 軽質転換効率
Research Abstract	In order to improve the system of genetic transformation in wheat, gene expression patterns of wheat cultured cells through transformation process were studied. Wheat cultivar "Bobwhite" which is the most suitable cultivar to transform was used as plant material. The change of gene expression levels caused by improved condition of transformation was analyzed using microarray. Expression variation of selected genes was compared with two varieties, Bobwhite and Nourin-61, by RT-PCR.

Report

(4 results)