Establishment of plant regeneration system from endosperm-derived cultures and analysis for endosperm development and regeneration by analyzing imprinting genes

• Project/Area Number: 20780016
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Horticulture/Landscape architecture
• Research Institution: Hokkaido University
• Principal Investigator: HOSHINO Yoichiro Hokkaido University, 北方生物圏フィールド科学センター, 助教 (50301875)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: クロミノウグイスカグラ / ハスカップ / 胚乳 / インプリント遺伝子 / 倍数性 / 培養 / FIE (fertilization independent endosperm) / 雄性配偶子 / FIE(fertilization independent endosperm)

Research Abstract

In this study, the novel culture procedure using endosperm has been developed in Lonicera caerulea. The cultured endosperm could regenerate into plantlets. The regenerated plants showed same ploidy level with endosperm. This means that endosperm culture is novel procedure for producing polyploid plants. To analyze regeneration ability from endosperm, genome imprinting has been focused. In this study, one of imprint genes, fertilization independent endosperm (FIE) was sequenced in the endosperm of L. caerulea. The regeneration machinery might be clarified by analyzing expression patterns of imprint genes during endosperm culture.

Research Products

• [Journal Article] Evaluation of the horticultural traits of genus Alstroemeria L. and genus Bomarea Mirb.(Alstroemeriaceae). (2011)
  • Author(s): Kashihara Y, Shinoda K, Murata N, Araki H, Hoshino Y
  • Journal Title: Turkish Journal of Botany 35 Pages: 239-245
  • Peer Reviewed
• [Journal Article] Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan. (2011)
  • Author(s): Miyashita T, Araki H, Hoshino Y
  • Journal Title: Journal of Plant Research 124 Pages: 1-9
  • NAID: 10027701364
  • URL: http://hdl.handle.net/2115/44725
  • Peer Reviewed
• [Journal Article] Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan. (2011)
  • Author(s): Miyashita T., Araki H., Hoshino Y.
  • Journal Title: Journal of Plant Research Volume: 124 Pages: 1-9
  • NAID: 10027701364
  • Peer Reviewed
• [Journal Article] Evaluation of the horticultural traits of genus Alstroemeria L.and genus Bomarea Mirb.(Alstroemeriaceae). (2011)
  • Author(s): Kashihara Y, Shinoda K, Murata N, Araki H, Hoshino Y
  • Journal Title: Turkish Journal of Botany Volume: 35 Pages: 239-245
  • Peer Reviewed
• [Journal Article] Capture of male gamete dynamics in pollen tubes.In : Pollen : Structure, Types and Effects. (2010)
  • Author(s): Hirano T, Hoshino Y
  • Journal Title: Nova Science Publishers, Inc.(Editors : Benjamin J.Kaiser.) Pages: 127-134
  • Peer Reviewed
• [Journal Article] Interspecific hybridization in Lonicera caerulea and Lonicera gracillipes : The occurrence of green/albino plants by reciprocal crossing. (2010)
  • Author(s): Miyashita T, Hoshino Y
  • Journal Title: Scientia Horticulturae 125 Pages: 692-699
  • URL: http://hdl.handle.net/2115/43307
  • Peer Reviewed
• [Journal Article] Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii. (2010)
  • Author(s): Hirano T, Hoshino Y
  • Journal Title: Sexual Plant Reproduction 23 Pages: 153-162
  • NAID: 120002834666
  • URL: http://hdl.handle.net/2115/44961
  • Peer Reviewed
• [Journal Article] Evaluation of pre-fertilization barriers by observation of pollen tube growth and attempts for overcoming post-fertilization barriers in intergeneric hybridization between Alstroemeria and Bomarea by ovule Culture. (2010)
  • Author(s): Kashihara Y, Hirano T, Murata N, Shinoda K, Araki H, Hoshino Y
  • Journal Title: Acta Horticulturae 855 Pages: 159-164
  • URL: http://hdl.handle.net/2115/44887
  • Peer Reviewed
• [Journal Article] Evaluation of pre-fertilization barriers by observation of pollen tube growth and attempts for overcoming post-fertilization barriers in intergeneric hybridization between Alstroemeria and Bomarea by ovule Culture. (2010)
  • Author(s): Kashihara Y., Hirano T., Murata N., Shinoda K., Araki H., Hoshino Y.
  • Journal Title: Acta Horticulturae Volume: 855 Pages: 159-164
  • Peer Reviewed
• [Journal Article] Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii. (2010)
  • Author(s): Hirano T., Hoshino Y.
  • Journal Title: Sexual Plant Reproduction Volume: 23 Pages: 153-162
  • NAID: 120002834666
  • Peer Reviewed
• [Journal Article] Interspecific hybridization in Lonicera caerulea and Lonicera gracillipes : The occurrence of green/albino plants by reciprocal crossing. (2010)
  • Author(s): Miyashita T., Hoshino Y.
  • Journal Title: Scientia Horticulturae Volume: 125 Pages: 692-699
  • Peer Reviewed
• [Journal Article] Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii. Sexual Plant Reproduction (2010)
  • Author(s): Hirano T., Hoshino Y.
  • Journal Title: Sexual Plant Reproduction (掲載確定)
  • Peer Reviewed
• [Journal Article] Ploidy distribution and DNA content variations of Lonicera caerulea(Caprifoliaceae)in Japan (2010)
  • Author(s): Miyashita T., Araki H., Hoshino Y.
  • Journal Title: Journal of Plant Research (掲載確定)
  • NAID: 10027701364
  • Peer Reviewed
• [Journal Article] Evaluation of pre-fertilization barriers by observation of pollen tube growth and attempts for overcoming post-fertilization barriers in intergeneric hybridization between Alstroemeria and Bomarea by ovule Culture (2010)
  • Author(s): Kashihara Y., Hirano T., Murata N., Shinoda K, Araki H., Hoshino Y.
  • Journal Title: Acta Horticulturae (掲載確定)
  • Peer Reviewed
• [Journal Article] Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var.emphyllocalyx. (2009)
  • Author(s): Miyashita T, Ohashi T, Shibata F, Araki H, Hoshino Y
  • Journal Title: Plant Cell, Tissue and Organ Culture 98 Pages: 291-301
  • URL: http://hdl.handle.net/2115/44168
  • Peer Reviewed
• [Journal Article] Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea. (2009)
  • Author(s): Hirano T, Hoshino Y
  • Journal Title: Journal of Plant Research 122 Pages: 225-234
  • NAID: 10025179739
  • URL: http://hdl.handle.net/2115/36650
  • Peer Reviewed
• [Journal Article] Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea (2009)
  • Author(s): Hirano T., Hoshino Y.
  • Journal Title: Journal of Plant Research 122 Pages: 225-234
  • NAID: 10025179739
  • Peer Reviewed
• [Journal Article] Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx (2009)
  • Author(s): Miyashita T., Ohashi T., Shibata F., Araki H., Hoshino Y.
  • Journal Title: Plant Cell, Tissue and Organ Culture 98 Pages: 291-301
  • NAID: 120002564616
  • Peer Reviewed
• [Journal Article] Plant regeneration from suspension cells induced from hypocotyls derived from interspecific cross Alstroemeria pelegrina×A.magenta and transformation with Agrobacterium tumefaciens. (2008)
  • Author(s): Hoshino Y, Kashihara Y, Hirano T, Murata N, Shinoda K
  • Journal Title: Plant Cell, Tissue and Organ Culture 94 Pages: 45-54
  • URL: http://hdl.handle.net/2115/38770
  • Peer Reviewed
• [Journal Article] Advances in Alstroemeria biotechnology. Floriculture, Ornamental and Plant Biotechnology : Advances and Topical Issues. (2008)
  • Author(s): Hoshino Y
  • Journal Title: Volume 5.Edited by Jaime A.Teixeira da Silva.Global Science Books, Middlesex, United Kingdom Pages: 540-547
  • URL: http://hdl.handle.net/2115/34118
  • Peer Reviewed
• [Presentation] ハスカップにおける倍数体シリーズの作出 (2010)
  • Author(s): 宮下朋美・中野英樹・高虫慧子・高橋太郎・堀廣孝・星野洋一郎
  • Organizer: 園芸学研究第9巻別冊2号p.412
  • Place of Presentation: 大分・大分大学
  • Year and Date: 2010-09-20
• [Presentation] キルタンサスにおける花粉管伸長過程での精細胞の異型化 (2010)
  • Author(s): 平野智也・星野洋一郎
  • Organizer: 園芸学研究第9巻別冊1号
  • Place of Presentation: 神奈川・日本大学
  • Year and Date: 2010-03-22
• [Presentation] Exploitation of wild Lonicera caerulea in Japan : ploidy distribution and DNA content variations. (2010)
  • Author(s): Hoshino Y, Miyashita T.
  • Organizer: 28th International Horticultural Congress.Lisbon, Portugal
  • Place of Presentation: ポルトガル・リスボン
• [Presentation] Exploitation of wild Lonicera caerulea in Japan : ploidy distribution and DNA content variations. (2010)
  • Author(s): Miyashita T., Hoshino Y.
  • Organizer: 28th International Horticultural Congress
  • Place of Presentation: Lisboa Congress Centre(ポルトガル、リスボン)
• [Presentation] ハスカップにおける倍数体シリーズの作出 (2010)
  • Author(s): 宮下朋美、中野英樹、高虫慧子、高橋太郎、堀廣孝、星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 大分大学(大分県)
• [Presentation] ハスカップ各系統における果実と葉のロガニン, キナ酸およびクエン酸含有量の評価 (2010)
  • Author(s): 桐山和也・本西誠亮・中野英樹・高虫慧子・堀廣孝・高橋太郎・荒木肇・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 日本大学湘南キャンパス、神奈川県
• [Presentation] キルタンサスにおける花粉管伸長過程での精細胞の異型化 (2010)
  • Author(s): 平野智也・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 日本大学湘南キャンパス、神奈川県
• [Presentation] 国内に自生するクロミノウグイスカグラの地理的分布およびその倍数性変異について (2009)
  • Author(s): 宮下朋美・柴田洋・桐山和也・荒木肇・星野洋一郎
  • Organizer: 園芸学研究第8巻別冊1号p.42
  • Place of Presentation: 東京・明治大学
  • Year and Date: 2009-03-19
• [Presentation] 国内に自生するクロミノウグイスカグラの地理的分布およびその倍数性変異について (2009)
  • Author(s): 宮下朋美・柴田洋・桐山和也・荒木肇・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 明治大学駿河台キャンパス(東京都)
  • Year and Date: 2009-03-19
• [Presentation] Evaluation of pre-fertilization barriers by observation of pollen tube growth and attempts for overcoming post-fertilization barriers in intergeneric hybridization between Alstroemeria and Bomarea by ovule culture (2009)
  • Author(s): Kashihara Y., Hirano T., Murata N., Shinoda K, Araki H., Hoshino, Y.
  • Organizer: 23^<rd> EUCARPIA SYMPOSIUM-Section Ornamentals "Colourful Breeding and Genetics"
  • Place of Presentation: オランダ、ライデン
• [Presentation] Production of triploid plants from crosses between diploid and tetraploid plants by ovule culture in Haskap (Lonicera caerulea var. emphyllocaryx) (2008)
  • Author(s): 宮下朋美・星野洋一郎
  • Organizer: Sixth International Symposium on In Vitro Culture and Horticultural Breeding
  • Place of Presentation: Chifley at Lennons, Bris bane, Australia
  • Year and Date: 2008-08-25
• [Book] Capture of male gamete dynamics in pollen tubes.(Pollen : Structure, Types and Effects) (2010)
  • Author(s): Hirano T, Hoshino Y
  • Publisher: Nova Science Publishers, Inc.