| Project/Area Number |
20780032
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant pathology
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKEMOTO Daigo Nagoya University, 大学院・生命農学研究科, 助教 (30456587)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 糸状菌 / 植物病原菌 / 植物共生菌 / 活性酸素生成酵素 / 細胞極性 / 共生菌 |
|---|
| Research Abstract |
Regulated synthesis of reactive oxygen species (ROS) by fungal NADPH oxidases (Nox) plays a key role in interactions of pathogenic and symbiotic fungi with host plants. In this study, we isolated and investigated the function of novel regulator of fungal Nox. Generation of ROS by fungal Nox enzymes requires assembly of a multi-subunit complex composed of NoxA, NoxR, and RacA. Interaction assays showed that NoxR interacts with RacA, BemA and Cdc24, and Cdc42 interacts with BeinA. BeinA, Cdc24, NoxR and RacA preferentially localized to hyphal tips. These results demonstrate that fungal BemA and Cdc24 play a critical role in localizing and activating Nox proteins, in order to establish polarity of fungal hyphal cells during the infection to host plants.
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