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Biocontrol activity of an endophytic Streptomyces sp. strain MBCN152-1 and its mode of action

Research Project

Project/Area Number 20780033
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionMie University

Principal Investigator

SHIMIZU Masafumi  Mie University, 大学院・生物資源研究科, 助教 (60378320)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords内生放線菌 / 生物防除 / 黒すす病 / 菌寄生 / 放線菌 / キャベツ / セル成型苗 / 植物内生 / Streptomyces / キャベツセル成型苗 / Alfernaria / 細胞壁分解酵素
Research Abstract

By scanning electron microscopy, it was revealed that Streptomyces sp. strain MBCN152-1 colonized cuticle surface and intercellular spaces of cabbage cotyledons. Light and fluorescent microscopic observation indicated that MBCN152-1 could discern A. brassicicola from other fungi, and parasitize hyphal cell wall of A. brassicicola. However, interestingly, MBCN152-1 exhibited intensive biocontrol effect against non-host pathogen Glomerella cingulata, causal agent of strawberry anthracnose. When cabbage seedlings colonized by MBCN152-1 were inoculated with A. brassicicola, infection by the pathogen was significantly suppressed, though formation of physical barrier such as papillae was not observed in the cells which were penetrated by infection hyphae of the pathogen. These results suggested that mycoparasitism and induction of systemic resistance without formation of physical barrier in the host plant cells were involved in the mechanisms of biocontrol by strain MBCN152-1.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (11 results)

All 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Book (2 results)

  • [Journal Article] A promising strain of endophytic Streptomyces sp. for biological control of cucumber anthracnose.2009

    • Author(s)
      Shimizu, M., Yazawa, S., Ushijima, Y.
    • Journal Title

      Journal of General Plant Pathology 75

      Pages: 27-36

    • NAID

      10029717207

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A promising strain of endophytic Streptomyces sp. for biological control of cucumber anthracnose2009

    • Author(s)
      Shimizu, M., Yazawa, S., Ushijima, Y.
    • Journal Title

      Journal of General Plant Pathology 75

      Pages: 27-36

    • NAID

      10029717207

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Streptomyces sp. MBCN152-1株に関する研究. (2)Alternaria brassicicolaに対するMBCN152-1株の寄生性.2009

    • Author(s)
      中山幹康・中筋智子・窪田昌春・鈴木智子・清水将文
    • Organizer
      平成21年度日本植物病理学会関西部会
    • Place of Presentation
      神戸市
    • Year and Date
      2009-10-17
    • Related Report
      2009 Final Research Report
  • [Presentation] Streptomyces sp.MBCN152-1株に関する研究.(2)Alternaria brassicicolaに対するMBCN152-1株の寄生性.2009

    • Author(s)
      中山幹康, ら
    • Organizer
      日本植物病理学会関西部会
    • Place of Presentation
      神戸市
    • Year and Date
      2009-10-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Hyperparasitism of Alternaria brassicicola by the biocontrol strain Streptomyces sp. MBCN152-1.2009

    • Author(s)
      Masafumi Shimizu, Mikiyasu Nakayama, Tomoko Suzuki.
    • Organizer
      The 2009 KSPP fall meeting and the 1st Japan-Korea joint symposium
    • Place of Presentation
      韓国済州島
    • Related Report
      2009 Final Research Report
  • [Presentation] Streptomyces sp. MBCN152-1株に関する研究. (1)キャベツセル成型苗黒すす病に対する生物防除活性.2009

    • Author(s)
      清水将文・中筋智子・中山幹康・窪田昌春・鈴木智子・山中聡
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      山形市
    • Related Report
      2009 Final Research Report
  • [Presentation] Hyperparasitism of Alternaria brassicicola by the biocontrol strain Streptomyces sp. MBCN152-1.2009

    • Author(s)
      Masafumi Shimizu, et al.
    • Organizer
      The 2009 KSPP fall meeting and the 1^<st> Japan-Korea joint symposium
    • Place of Presentation
      韓国済州島
    • Related Report
      2009 Annual Research Report
  • [Presentation] Streptomyces sp. MBCN152-1株に関する研究.(1)キャベツセル成型苗黒すす病に対する生物防除活性2009

    • Author(s)
      清水将文・中筋智子・中山幹康・窪田昌春・鈴木智子・山中聡
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形市
    • Related Report
      2008 Annual Research Report
  • [Presentation] ウリ類炭疽病に対する放線菌殺菌剤の開発〜有望内生放線菌株の探索〜2008

    • Author(s)
      清水将文・矢澤佐知子・牛島悠介
    • Organizer
      2008年度放線菌学会大会
    • Place of Presentation
      山梨市
    • Related Report
      2008 Annual Research Report
  • [Book] 微生物と植物の相互作用:病害と生物防除(分担:内生放線菌による誘導抵抗)2009

    • Author(s)
      清水将文
    • Publisher
      ソフトサイエンス社
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Book] 微生物と植物の相互作用-病害と生物防除-2009

    • Author(s)
      清水将文, 他49名
    • Publisher
      ソフトサイエンス社
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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