Yeast two-hybrid study of BmMLV protein and host protein interactions
Project/Area Number |
20780035
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Applied entomology
|
Research Institution | Utsunomiya University |
Principal Investigator |
IWANAGA Masashi Utsunomiya University, 農学部, 准教授 (40400717)
|
Project Period (FY) |
2008 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | ウイルス / 培養細胞 / 持続感染 / カイコ / 感染性クローン / 相互作用 / ヘリカーゼ / two-hybrid / 遺伝子 / タンパク質 |
Research Abstract |
Bombyx mori macula-like virus (BmMLV) persistently infects to B. mori-derived cell lines. However, the mechanism of virus replication is unknown. To clarify this, yeast two-hybrid screening was performed and showed that the interaction of BmMLV HELICASE and several translational factor of B. mori. Also, the screening showed that the dimerization of BmMLV HELICASE. In addition, we developed an infectious full-length cDNA clone of BmMLV.
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Report
(3 results)
Research Products
(12 results)