Effect of heavy-ion irradiation on insect cells

• Project/Area Number: 20780037
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: FUKAMOTO Kana Japan Atomic Energy Agency, 繊維学部, アソシエイト研究員 (40435607)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: カイコ / 昆虫細胞 / 重イオンビーム / 障害修復 / アポトーシス / 放射線障害修復 / 発生初期卵 / 放射線耐性 / Sf9細胞 / 昆虫

Research Abstract

Insect cells are considered to be more resistant to radiation compared with mammalian cells, but the detail mechanisms of this resistance have not been understood. To clarify the molecular mechanisms of radioresistance in insect cells to heavy ions, we investigated the effect of carbon ion (^<12>C^<5+>) irradiation on insect cells (Sf9). As a result, no effects of carbon ion irradiation on the viability of Sf9 cells were observed even at a dose of 400Gy. However, the dose-dependent inihibition of cell proliferation was found. These results indicate the insect cells are relatively high resistant to radiation with mammalian cells.

Research Products

• [Journal Article] Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells. (2010)
  • Author(s): Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Mutou Y, Kobayashi Y, Yorifuji H.
  • Journal Title: J.Electron Microsc. 59 Pages: 495-501
  • Peer Reviewed
• [Journal Article] Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells. (2010)
  • Author(s): Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Mutou Y, Kobayashi Y, Yorifuji H.
  • Journal Title: J Electron Microsc Volume: 59 Pages: 495-501
  • Peer Reviewed
• [Journal Article] Targeted heavy-ion microbeam irradiation of the embryo but not yolk in the diapause-terminated egg of the silkworm, Bombyx mori, induces the somatic mutation. (2009)
  • Author(s): Furusawa T, Fukamoto K, Sakashita T, Suzuki E, Kakizaki T, Hamada N, Funayama T, Suzuki H, Ishioka N, Wada S, Kobayashi Y, Nagaoka S.
  • Journal Title: J.Radiat.Res. 50 Pages: 371-375
  • NAID: 10025912407
  • Peer Reviewed
• [Journal Article] Insufficient membrane fusion in dysferlin-deficient muscle fibers after heavy-ion irradiation. (2009)
  • Author(s): Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Kobayashi Y, Yorifuji H.
  • Journal Title: Cell Struct.Funct 34 Pages: 11-15
  • NAID: 130004137540
  • Peer Reviewed
• [Journal Article] Effects of ionizing radiation on locomotory behavior and mechanosensation in Caenorhabditis elegans. (2009)
  • Author(s): Suzuki M, Sakashita T, Yanase S, Kikuchi M, Ohba H, Higashitani A, Hamada N, Funayama T, Fukamoto K, Tsuji T, Kobayashi Y.
  • Journal Title: J.Radiat.Res. 50 Pages: 119-125
  • NAID: 10025911390
  • Peer Reviewed
• [Journal Article] Targeted heavy-ion microbeam irradiation of the embryo but not yolk in the diapause-terminated egg of the silkworm, Bombyx mori, induces the somatic mutation. (2009)
  • Author(s): T.Furusawa
  • Journal Title: Journal of Radiation Research 50 Pages: 371-375
  • Peer Reviewed
• [Journal Article] Effects of Ionizing Radiation on Locomotory Behavior andMechanosensation in Caenorhabditis elegans. (2009)
  • Author(s): M.Suzuki
  • Journal Title: Journal of Radiation Research 50 Pages: 161-169
  • Peer Reviewed
• [Journal Article] Insufficient membrane fusion in dysferlin-deficient muscle fibers after heavy-ion irradiation. (2009)
  • Author(s): M.Hino
  • Journal Title: Cell Structure and Function 34 Pages: 11-15
  • Peer Reviewed
• [Journal Article] Heavy-ion microbeam system at JAEA-Takasaki for microbeam biology. (2008)
  • Author(s): Funayama T, Wada S, Yokota Y, Fukamoto K, Sakashita T, Taguchi M, Kakizaki T, Hamada N, Suzuki M, Furusawa Y, Watanabe H, Kiguchi K, Kobayashi Y.
  • Journal Title: J.Radia.t Res. 49 Pages: 71-82
  • NAID: 110006570530
  • Peer Reviewed
• [Journal Article] Heavy-ion microbeam system at JAEA-Takasaki for microbe am biology (2008)
  • Author(s): Tomoo Funayama
  • Journal Title: Journal of Radiation Research 49 Pages: 71-82
  • Peer Reviewed
• [Journal Article] 重イオンマイクロビームを用いた生物学研究 (2008)
  • Author(s): 小林泰彦
  • Journal Title: 放射線生物研究 43 Pages: 151-169
• [Presentation] 重イオン照射造血器官の崩壊・再生機構:血球前駆細胞の重イオンに対する応答 (2010)
  • Author(s): 深本花菜・小林智史・土屋志織・舟山知夫・横田裕一郎・坂下哲哉・小林泰彦・木口憲爾・白井孝治
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学
  • Year and Date: 2010-04-03
• [Presentation] エビガラスズメ緑色幼虫の体色発現機構:色素顆粒中のINS凝集成分X について (2010)
  • Author(s): 白井孝治・福島壽斗・片桐千仭・深本花菜・木口憲爾
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学
  • Year and Date: 2010-04-03
• [Presentation] 重イオン照射造血器官の崩壊・再生機構:血球前駆細胞の重イオンに対する応答 (2010)
  • Author(s): 深本花菜・小林智史・土屋志織・舟山知夫・横田裕一郎・坂下哲哉・小林泰彦・木口憲爾・白井孝治
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2010-04-03
• [Presentation] エビガラスズメ緑色幼虫の体色発現機構:色素顆粒中の工NS凝集成分Xについて (2010)
  • Author(s): 白井孝治・福島壽斗・片桐千仭・深本花菜・木口憲爾
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2010-04-03
• [Presentation] 重イオン照射カイコ造血器官の崩壊・再生機構の解明-低線量照射における影響- (2009)
  • Author(s): 小林智史・深本花菜・白井孝治・木口憲爾・舟山知夫・坂下哲哉・小林泰彦
  • Organizer: 日本蚕糸学会中部支部第65回・東海支部第57回合同研究発表会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-12-03
• [Presentation] 重イオンビーム照射カイコ造血器官の崩壊・再生機構の解明-低線量照射における影響- (2009)
  • Author(s): 小林智史・深本花菜・白井孝治・木口憲爾・舟山知夫・坂下哲哉・小林泰彦
  • Organizer: 日本蚕糸学会中部支部第65回・東海支部第57回合同研究発表会
  • Place of Presentation: 名古屋大学野依記念学術交流会館
• [Presentation] 昆虫培養細胞Sf9におけるバイスタンター効果の有無について (2008)
  • Author(s): 土屋志織
  • Organizer: 日本蚕糸学会中部支部会
  • Place of Presentation: 長野県上田市
  • Year and Date: 2008-11-27
• [Presentation] 重イオン照射造血器官の崩壊・再生機構-崩壊期における eIF2 α kinase (BeK)の発現の調査- (2008)
  • Author(s): 小林智史
  • Organizer: 日本蚕糸学会中部支部会
  • Place of Presentation: 長野県上田市
  • Year and Date: 2008-11-27
• [Presentation] 重イオン照射造血器官の崩壊・再生機構-崩壊期におけるeIF2αキナーゼ(BeK)の発現調査 (2008)
  • Author(s): 小林智史・土屋志織・白井孝治・木口憲爾・深本花菜・横田裕一郎・舟山知夫・坂下哲哉・小林泰彦
  • Organizer: 日本蚕糸学会中部支部第64回・東海支部第56回合同研究発表会
  • Place of Presentation: 信州大学
• [Presentation] 昆虫培養細胞Sf9におけるバイスタンダー効果の有無について (2008)
  • Author(s): 土屋志織・白井孝次・木口憲爾・深本花菜・横田裕一郎・舟山知夫・坂下哲哉・小林泰彦
  • Organizer: 日本蚕糸学会中部支部第64回・東海支部第56回合同研究発表会
  • Place of Presentation: 信州大学
• [Remarks] ホームページ
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