Basic Study for Developing Silk Protein Containing Unnatural Functional Groups
| Project/Area Number |
20780039
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
TERAMOTO Hidetoshi National Institute of Agrobiological Sciences, 絹タンパク素材開発ユニット, 主任研究員 (60391562)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | シルク / カイコ / 培養細胞 / 非天然アミノ酸 / フェニルアラニル-tRNA / 合成酵素 / フェニルアラニルーtRNA合成酵素 / フェニルアラニル-tRNA合成酵素 |
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| Research Abstract |
The genes encoding phenylalanyl-tRNA synthetase (PheRS) were cloned from the silk glands of silkworm, Bombyx mori. Based on the sequence comparison with PheRSs from other organisms, residues responsible for discriminating phenylalanine (Phe) from others were identified, and these residues were mutated to amino acids with smaller side chains to obtain five mutant PheRS genes. In vitro assays revealed that the PheRS mutant with Ala 450 to Gly mutation recognizes Phe analogs bearing Cl and Br at the para position of the phenyl ring.
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Report
(3 results)
Research Products
(5 results)
