Development of a new insect pest control method targeting to insect sex determination genes

• Project/Area Number: 20780041
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SUZUKI MasatakaG. The Institute of Physical and Chemical Research, 大学院・新領域創成科学研究科, 准教授 (30360572)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 性決定 / doublesex / 選択的スプライシング / カイコ / 性決定遺伝子 / Bmdsx / transformer2 / Bmtra2 / BmPSI / KHドメイン / 性決定遣伝子

Research Abstract

The functional consequence of the interaction between MSP55 and BmPSI was examined using RNA mobility shift analysis. MSP55 increased BmPSI RNA binding activity by decreasing the rate of BmPSI dissociation from target RNA. Truncation analysis of MSP55 indicated that KH type RNA binding domains appeared to be responsible for enhancing BmPSI RNA binding activity.

Research Products

• [Journal Article] Identification of a male-specific RNA binding protein that regulates sex-specific splicing of Bmdsx by increasing RNA binding activity of BmPSI. (2010)
  • Author(s): Suzuki, M.G., Imanishi, S., Dohmae, N., Nishimura, T., Matsumoto, S.
  • Journal Title: Mol.Cell. iol. 30 Pages: 5776-5886
  • Peer Reviewed
• [Journal Article] Sex determination : insights from the silkworm. (2010)
  • Author(s): Masataka G.Suzuki
  • Journal Title: Journal of Genetics 89(3) Pages: 357-363
• [Journal Article] Sex determination : insights from the silkworm. (2010)
  • Author(s): Masataka G.Suzuki
  • Journal Title: Journal of Genetics Volume: 89 Pages: 357-363
  • Peer Reviewed
• [Journal Article] Identification of a male-specific RNA binding protein that regulates sex-specific splicing of Bmdsx by increasing RNA binding activity of BmPSI. (2010)
  • Author(s): Masataka G.Suzuki
  • Journal Title: Molecular and Cellular Biology Volume: 30 Pages: 5776-5886
  • Peer Reviewed
• [Journal Article] 昆虫の性決定機構-性決定の鍵を握るスプライシング (2009)
  • Author(s): 鈴木雅京
  • Journal Title: 生物の科学 遺伝 63 Pages: 49-56
• [Journal Article] Establishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons. (2008)
  • Author(s): Suzuki, M.G., Imanishi, S., Dohmae, N., Nishimura, T., Shimada, T., Matsumoto, S.
  • Journal Title: Mol.Cell.Biol. 28 Pages: 333-343
  • Peer Reviewed
• [Journal Article] Establishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons (2008)
  • Author(s): Masataka G. Suzuki
  • Journal Title: Molecular and Cellular Biology 28 Pages: 333-343
  • Peer Reviewed
• [Presentation] オス特異的RNA結合タンパク質MSP55によるBmdsxの性特異的スプライシング制御 (2010)
  • Author(s): 鈴木雅京、今西重雄、松本正吾
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学農学部
  • Year and Date: 2010-04-03
• [Presentation] オス特異的RNA結合タンパク質MSP55によるBmdsxの性特異的スプライシング制御 (2010)
  • Author(s): 鈴木雅京
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 長野県信州大学農学部
  • Year and Date: 2010-04-03
• [Presentation] tra2のカイコホモログBmtra2の機能解析 (2010)
  • Author(s): 鈴木啓二、青木不学、鈴木雅京
  • Organizer: 日本蚕糸学会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2010-03-21
• [Presentation] 昆虫培養細胞を利用した性決定機構の解析 (2010)
  • Author(s): 鈴木雅京、松本正吾
  • Organizer: バイオアーキテクト研究推進グループ
  • Place of Presentation: 静岡県熱海市
  • Year and Date: 2010-02-24
• [Presentation] Bmdsxの性特異的スプライシングに関わるオス特異的RNA結合タンパク質 (2009)
  • Author(s): 鈴木雅京、今西重雄、松本正吾
  • Organizer: 日本蚕糸学会
  • Place of Presentation: 東京農工大学農学部
  • Year and Date: 2009-03-21
• [Book] 生物の科学遺伝,63(1)(昆虫の性決定機構-性決定の鍵を握るスプライシング) (2009)
  • Author(s): 鈴木雅京
• [Remarks] ホームページ等
  • URL: http://park.itc.u-tokyo.ac.jp/seigyo/index.html
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/seigyo/index.html
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/research/lab/wako/entomology/index.html
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/research/lab/wako/entomology/index.html