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Investigation into the molecular mechanisms of reproductive manipulation of host insect by endosymbiotic bacteria.

Research Project

Project/Area Number 20780042
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied entomology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

ANBUTSU Hisashi  National Institute of Advanced Industrial Science and Technology, 生物プロセス研究部門, 研究グループ付 (30392583)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords共生細菌 / ショウジョウバエ / スピロプラズマ / ファージ / 生殖操作 / 雄殺し / 免疫機構 / 性比 / P58遺伝子ファミリー / 抗菌タンパク質 / 分子機構
Research Abstract

This study has been conducted using the endosymbiotic system consists of host insects, Drosophila fruit flies, and symbiotic bacteria, Spiroplasma, which cause "male killing" on its hosts. From the three Spiroplasma strains, genome sequences of their phages were identified and characterized. It was shown that Spiroplasma infections neither induced nor suppressed the systemic immune response of the host. In addition, it was also determined that the male-killing Spiroplasma is capable of suppressing the constitutive immune response and is resistant to mounted immune attacks. Sixteen insertion lines, which showed extremely male-biased sex ratio, have been obtained and the possible causative genes have been identified.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (12 results)

All 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] Spiroplasma as a model insect endosymbiont2011

    • Author(s)
      安佛尚志、深津武馬
    • Journal Title

      Environmental Microbiology Reports Vo1.3, No.2

      Pages: 144-153

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spiroplasma as a model insect endosymbiont2011

    • Author(s)
      安佛尚志・深津武馬
    • Journal Title

      Environmental Microbiology Reports

      Volume: VOL.3 Pages: 144-153

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evasion, suppression and tolerance of Drosophila innate immunity by a male-killing Spiroplasma endosymbiont2010

    • Author(s)
      安佛尚志、深津武馬
    • Journal Title

      Insect Molecular Biology. Vol.19, No.4

      Pages: 481-488

    • Related Report
      2010 Final Research Report
  • [Journal Article] Evasion, suppression and tolerance of Drosophila innate immuuity by a male-killing Spiroplasma endosymbiont2010

    • Author(s)
      安佛尚志・深津武馬
    • Journal Title

      Insect Molecular Biology

      Volume: VOL.19 Pages: 481-488

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 宿主昆虫の生殖を操作する共生細菌スピロプラズマ2010

    • Author(s)
      安佛尚志
    • Organizer
      第37回日本マイコプラズマ学会学術集会
    • Place of Presentation
      国立感染症研究所(東京都)
    • Year and Date
      2010-06-10
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Infection dynamics and immune resistance of male-killing and non-male-killing spiroplasmas2010

    • Author(s)
      安佛尚志、深津武馬
    • Organizer
      Symposium "Microbial Interactions Leading to Novel Biological Functions"
    • Place of Presentation
      産業技術総合研究所(茨城県つくば市)
    • Year and Date
      2010-01-08
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Expression of antimicrobial peptide genes in Drosophila infected with Spiroplasma endosymbiont and its effect on infection dynamics2009

    • Author(s)
      安佛尚志、深津武馬
    • Organizer
      The 9th Japanese Drosophila Research Conference
    • Place of Presentation
      ヤマハリゾートつま恋(静岡県掛川市)
    • Year and Date
      2009-07-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Expression of Antimicrobial Peptide Genes in Drosophila Infected with Spiroplasma Endosymbiont and Its Effect on Infection Dynamics2009

    • Author(s)
      安佛尚志・深津武馬
    • Organizer
      The 9th Japanese Drosophila Research Conference
    • Place of Presentation
      ヤマハリゾートつま恋(静岡県掛川市)
    • Year and Date
      2009-07-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] ショウジョウバエ・スピロプラズマ共生系を用いて昆虫内部共生のメカニズムを探る2009

    • Author(s)
      安佛尚志
    • Organizer
      日本節足動物発生学会第45回大会
    • Place of Presentation
      ホテルニュー白亜紀(茨城県ひたちなか市)
    • Year and Date
      2009-06-05
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] なぜ共生細菌スピロプラズマは宿主の免疫システムによって排除されないのか2009

    • Author(s)
      安佛尚志
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] なぜ共生細菌スピロプラズマは宿主の免疫システムによって排除されないのか2009

    • Author(s)
      安佛 尚志
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Remarks] 産総研論文検索システム(産総研リポジトリ)

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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