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Molecular genetic analysis of Rubisco degradation to maintain leafphotosynthesis

Research Project

Project/Area Number 20780044
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

ISHIDA Hiroyuki  Tohoku University, 大学院・農学研究科, 准教授 (60312625)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsRubisco / 老化 / オートファジー / 葉緑体 / 植物
Research Abstract

I performed genetic analysis of the degradation mechanism of CO2-fixing enzyme, Rubisco, in senescing leaves, which is closely related to plant growth through photosynthesis and nutrient recycling. I isolated mutants which show abnormal stromule formation and mutants which delay the decrease in Rubisco content during leaf senescence. In addition, I revealed the mechanism of Rubisco degradation by autophagy.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (29 results)

All 2010 2009 2008

All Journal Article (15 results) (of which Peer Reviewed: 12 results) Presentation (14 results)

  • [Journal Article] Biochemical changes associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions.2010

    • Author(s)
      Nakano, R., Ishida, H., Kobayashi, M., Makino, A., Mae, T.
    • Journal Title

      Plant Biol. 12

      Pages: 35-45

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical changes associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions2010

    • Author(s)
      Nakano, R., Ishida, H., Kobayashi, M., Makino, A., Mae, T.
    • Journal Title

      Plant Biol. 12

      Pages: 35-45

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplasts autophagy during senescence of individually darkened leaves.2009

    • Author(s)
      Wada S., Ishida, H.
    • Journal Title

      Plant Signal Behav. 4

      Pages: 565-567

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy of whole and partial chloroplasts in individually darkened leaves: a unique system in plants?2009

    • Author(s)
      Ishida, H., Wada, S.
    • Journal Title

      Autophagy 5

      Pages: 736-737

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy plays a role in chloroplast degradation during senescence in individually darkened leaves.2009

    • Author(s)
      Wada, S., Ishida, H., Izumi, M., Yoshimoto, K., Ohsumi, Y., Mae, T., Makino, A.
    • Journal Title

      Plant Physiol. 149

      Pages: 885-893

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy of whole and partial chloroplasts in individually darkened leaves : a unique system in plants?2009

    • Author(s)
      Ishida, H., Wada, S.
    • Journal Title

      Autophagy 5

      Pages: 736-737

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplasts autophagy during senescence of individually darkened leaves2009

    • Author(s)
      Wada S., Ishida, H.
    • Journal Title

      Plant Signal Behav. 4

      Pages: 565-567

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplasts are partially mobilized to the vacuole by autophagy.2008

    • Author(s)
      Ishida, H., Yoshimoto, K.
    • Journal Title

      Autophagy 4

      Pages: 961-962

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mobilization of Rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process.2008

    • Author(s)
      Ishida, H., Yoshimoto, K., Izumi, M., Reisen, D., Yano, Y., Makino, A., Ohsumi, Y., Hanson, M.R., Mae, T.
    • Journal Title

      Plant Physiol. 148

      Pages: 142-155

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 葉緑体タンパク質の分解とオートファジー2008

    • Author(s)
      石田宏幸、和田慎也
    • Journal Title

      光合成研究 53

      Pages: 89-94

    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Journal Article] Rubisco and photosynthesis in cereal crops.2008

    • Author(s)
      Makino, A., Ishida, H.
    • Journal Title

      Tohoku Journal of Agricultural Research. 58

      Pages: 127-135

    • Related Report
      2009 Final Research Report
  • [Journal Article] Mobilization of Rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process2008

    • Author(s)
      Ishida, H., et.al.
    • Journal Title

      Plant Physiology 148

      Pages: 142-155

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplasts are partially mobilized to the vacuole by autophagy2008

    • Author(s)
      Ishida, H. and Yoshimoto, K.
    • Journal Title

      Autophagy 4

      Pages: 961-962

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy plays a role in chloroplast degradation during senescence in individually darkened leaves2008

    • Author(s)
      Wada, S., Ishida, H., et.al.
    • Journal Title

      Plant Physiology 149

      Pages: 885-893

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rubisco and photosynthesis in cereal crops2008

    • Author(s)
      Makino, A. and Ishida, H.
    • Journal Title

      Tohoku Journal of Agricultural Research 58

      Pages: 127-135

    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナ切離葉における栄養要因がRCBの形成に及ぼす影響の解析2010

    • Author(s)
      泉正範、石田宏幸、牧野周
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-20
    • Related Report
      2009 Final Research Report
  • [Presentation] シロイヌナズナにおけるデンプン代謝の変異がRCBの形成に及ぼす影響の解析2010

    • Author(s)
      泉正範、石田宏幸、牧野周
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] The role of autophagy in nutrient starvation and aging.2009

    • Author(s)
      Yoshimoto, K., Ishida, H., Wada, S., Ohsumi, Y., Shirasu, K.
    • Organizer
      5th International Symposium on Autophagy
    • Place of Presentation
      大津
    • Year and Date
      2009-09-24
    • Related Report
      2009 Final Research Report
  • [Presentation] The analysis of factors affecting the creation of Rubisco-containing bodies(RCBs), a specific autophagic process for chloroplast degradation in plants.2009

    • Author(s)
      Izumi, M., Ishida, H., Makino, A.
    • Organizer
      5th International Symposium on Autophagy
    • Place of Presentation
      大津
    • Year and Date
      2009-09-24
    • Related Report
      2009 Final Research Report
  • [Presentation] The chloroplast degradation in two different pathways in individually darkened leaves of Arabidopsis by autophagy.2009

    • Author(s)
      Wada, S., Ishida, H., Yoshimoto, K., Ohsumi, Y., Makino, A.
    • Organizer
      5th International Symposium on Autophagy
    • Place of Presentation
      大津
    • Year and Date
      2009-09-24
    • Related Report
      2009 Final Research Report
  • [Presentation] シロイヌナズナ切離葉における栄養要因がRCBの形成に及ぼす影響の解析2009

    • Author(s)
      泉正範、石田宏幸、牧野周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] シロイヌナズナの切離葉におけるRCBの形成に影響を及ぼす要因の解析2009

    • Author(s)
      泉正範、石田宏幸、牧野周
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-22
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] シロイヌナズナ個別暗処理葉におけるオートファジーに依存した葉緑体の分解2009

    • Author(s)
      和田慎也、石田宏幸, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-22
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] The role of autophagy in nutrient starvation and aging2009

    • Author(s)
      Yoshimoto, K., Ishida, H., et al
    • Organizer
      International Symposium on Autophagy
    • Place of Presentation
      大津プリンスホテル
    • Related Report
      2009 Annual Research Report
  • [Presentation] The analysis of factors affecting the creation of Rubisco-containing bodies(RCBs), a specific autophagic process for chloroplast degradation in plants2009

    • Author(s)
      泉正範, 石田宏幸, 牧野周
    • Organizer
      International Symposium on Autophagy
    • Place of Presentation
      大津プリンスホテル
    • Related Report
      2009 Annual Research Report
  • [Presentation] he chloroplast degradation in two different pathways in individually darkened leaves of Arabidopsis by autophagy2009

    • Author(s)
      Wada, S., Ishida, H., et al
    • Organizer
      International Symposium on Autophagy
    • Place of Presentation
      大津プリンスホテル
    • Related Report
      2009 Annual Research Report
  • [Presentation] 膜小胞RCBを介した液胞におけるRubiscoの分解2008

    • Author(s)
      泉正範、石田宏幸、牧野周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2008-09-09
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] シロイヌナズナの個葉・個体暗処理による老化誘導とオートファジーによる葉緑体分解2008

    • Author(s)
      和田慎也、石田宏幸, 他
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2008-09-09
    • Related Report
      2009 Final Research Report
  • [Presentation] シロイネナズナの個葉・個体暗処理による老化誘導とオートファジーによる葉緑体分解2008

    • Author(s)
      和田慎也、石田宏幸、他
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2008-09-09
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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