Study of metal regulation mechanism at reproductive stage of rice plant

• Project/Area Number: 20780047
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science
• Research Institution: Utsunomiya University
• Principal Investigator: MICHIKO Takahashi Utsunomiya University, 農学部, 准教授 (90345182)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物 / 発生・分化 / 発現制御 / 金属栄養 / ストレス

Research Abstract

To find important genes expressed during embryogenesis of rice plant, microarray analysis was performed using ovary before and after anthesis and during seed development. In this study, we illuminated the importance of metal during embryogenesis and pollen germination of rice plant.

Research Products

• [Journal Article] 高等植物におけるニコチアナミンの機能解析と有用作物の作出 (2010)
  • Author(s): 高橋美智子
  • Journal Title: 農林水産技術研究ジャーナル 33巻 Pages: 54-57
  • NAID: 10025999576
• [Journal Article] Rice metal-nicotianamine transporter, OsYSL2, is required for long distance transport of iron and manganese. (2010)
  • Author(s): Ishimaru Y, Masuda H, Bashir K, Inoue H, Tsukamoto T, Takahashi M, Nakanishi H, Aoki N, Hirose T, Ohsugi R, Nishizawa NK.
  • Journal Title: Plant J. (in press)
  • Peer Reviewed
• [Journal Article] 高等植物におけるニコチアナミンの機能解析と有用作物の作出 (2010)
  • Author(s): 高橋美智子
  • Journal Title: 農林水産技術研究ジャーナル 33 Pages: 54-57
  • NAID: 10025999576
• [Journal Article] Rice metal-nicotianamine transporter, OsYSL2, is required for long distance transport of iron and manganese (2010)
  • Author(s): Ishimaru Y., Masuda H., Bashir K., Inoue H., Tsukamoto T., Takahashi M., Nakanishi H., Aoki N., Hirose T., Ohsugi R., Nishizawa NK.
  • Journal Title: Plant J. (In press)
  • Peer Reviewed
• [Journal Article] In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu. (2009)
  • Author(s): Takahashi, M., Nozoye, T., Kitajima. N., Fukuda, N., Hokura, A., Terada, Y., Nakai, I., Ishimaru, Y., Kobayashi, T., Nakanishi, H., Nishizawa, NK.
  • Journal Title: Plant Soil 325巻 Pages: 39-51
  • Peer Reviewed
• [Journal Article] OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints. (2009)
  • Author(s): Aoyama T, Kobayashi T, Takahashi M, Nagasaka S, Usuda K, Kakei Y, Ishimaru Y, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Plant Mol. Biol. 70巻 Pages: 681-692
  • Peer Reviewed
• [Journal Article] The rice transcription factor IDEF1 is essential for the early response to iron deficiency and induces vegetative expression of late embryogenesis abundant genes. (2009)
  • Author(s): Kobayashi, T., Nakanishi I.R., Ogo, Y., Kakei, Y., Nakanishi, H., Takahashi, M., Nishizawa, NK.
  • Journal Title: Plant J. 60巻 Pages: 948-961
  • Peer Reviewed
• [Journal Article] Time course analysis of gene expression over 24 hours in Fe-deficient barley roots. (2009)
  • Author(s): Nagasaka S, Takahashi M, Itai RN, Bashir K, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Plant Mol. Biol. 69巻 Pages: 621-631
  • Peer Reviewed
• [Journal Article] A highly sensitive, quick, and simple quantification method for nicotianamine and 2'-deoxymugineic acid from minimum samples using LC/ESI-TOF-MS achieves functional analysis of these components in plants. (2009)
  • Author(s): Inoue H, Kobayashi T, Nozoye T, Takahashi M, Kakei Y, Suzuki K, Nakazono M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: J. Biol. Chem. 284巻 Pages: 3470-3479
  • NAID: 10027344558
  • Peer Reviewed
• [Journal Article] A highly sensitive, quick, and simple quantification method for nicotianamine and 2'-deoxymugineic acid from minimum samples using LC/ESI-TOF-MS achieves functional analysis of these components in plants. (2009)
  • Author(s): Kakei Y, Yamaguchi I, Kobayashi T, Takahashi M, Nakanishi H, Yamakawa T, Nishizawa NK.
  • Journal Title: Plant Cell Physiol. 50巻 Pages: 1988-1993
  • NAID: 10027344558
  • Peer Reviewed
• [Journal Article] Overexpression of the barley nicotianamine synthase gene HvNAS1 increases iron and zinc concentrations in rice grains. (2009)
  • Author(s): Masuda H, Usuda K, Kobayashi T, Ishimaru Y, Kakei Y, Takahashi M, Higuchi K, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Rice 2巻 Pages: 155-166
  • Peer Reviewed
• [Journal Article] In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu (2009)
  • Author(s): Takahashi, M., Nozoye, T., Kitajima, N., Fukuda, N., Hokura, A., Terada, Y., Nakai, I., Ishimaru, Y., Kobayashi, T., Nakanishi, H., Nishizawa, NK.
  • Journal Title: Plant Soil 325 Pages: 39-51
  • Peer Reviewed
• [Journal Article] OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints (2009)
  • Author(s): Aoyama T., Kobayashi T., Takahashi M., Nagasaka S., Usuda K., Kakei Y., Ishimaru Y., Nakanishi H., Mori S., Nishizawa NK.
  • Journal Title: Plant Mol.Biol. 70 Pages: 681-692
  • Peer Reviewed
• [Journal Article] The rice transcription factor IDEF1 is essential for the early response to iron deficiency and induces vegetative expression of late embryogenesis abundant genes (2009)
  • Author(s): Kobayashi, T., Nakanishi I.R., Ogo, Y., Kakei, Y., Nakanishi, H., Takahashi, M., Nishizawa, NK.
  • Journal Title: Plant J. 60 Pages: 948-961
  • Peer Reviewed
• [Journal Article] Time course analysis of gene expression over 24 hours in Fe-deficient barley roots (2009)
  • Author(s): Nagasaka S, Takahashi M, Itai RN, Bashir K, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Plant Mol.Biol. 169 Pages: 621-631
  • Peer Reviewed
• [Journal Article] Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings (2009)
  • Author(s): Inoue H., Kobayashi T., Nozoye T., Takahashi M., Kakei Y., Suzuki K., Nakazone M., Nakanishi H., Mori S., Nishizawa NK.
  • Journal Title: J.Biol.Chem. 284 Pages: 3470-3479
  • Peer Reviewed
• [Journal Article] A highly sensitive, quick, and simple quantification method for nicotianamine and 2'-deozymugineic acid from minimum samples using LC/ESl-TOF-MS achieves functional analysis of these components in plants (2009)
  • Author(s): Kakei Y, Yamaguchi I, Kobayashi T, Takahashi, M, Nakanishi H, Yamakawa T, Nishizawa NK.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1988-1993
  • Peer Reviewed
• [Journal Article] Overexpression of the barley nicotianamine synthase gene HvNAS1 increases iron and zinc concentrations in rice grains (2009)
  • Author(s): Masuda H., Usuda K, Kobayashi T., Ishimaru Y, Kakei Y, Takahashi M, Higuchi K, Nakanishi H., Mori S, Nishizawa NK.
  • Journal Title: Rice 2 Pages: 155-166
  • Peer Reviewed
• [Journal Article] A novel transcription factor IDEF2 that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants. (2008)
  • Author(s): Ogo Y, Kobayashi T, Nakanishi Itai R, Nakanishi H, Kakei Y, Takahashi M, Toki S, Mori S, Nishizawa NK.
  • Journal Title: J. Biol. Chem. 283巻 Pages: 13407-13417
  • Peer Reviewed
• [Journal Article] Deoxymugineic acid increases Zn translocation in Zn-deficient rice plants. (2008)
  • Author(s): Suzuki M, Tsukamoto T, Inoue H, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Plant Mol. Biol. 66巻 Pages: 609-617
  • Peer Reviewed
• [Journal Article] Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants. (2008)
  • Author(s): Ishimaru Y, Suzuki M, Ogo Y, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
  • Journal Title: Soil Sci. Plant Nutr. 54巻 Pages: 417-423
  • NAID: 110007326872
  • Peer Reviewed
• [Journal Article] A novel transcription factor IDEF2 that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants. (2008)
  • Author(s): Ogo Y, Kobayashi T, Nakanishi Itai R, Nakanishi H, Ka kei Y. Takahashi M. Told S. Mori S. Nishizawa NK
  • Journal Title: Journal of Biological Chemistry (in press)
  • Peer Reviewed
• [Journal Article] Deoxymugineic acid increases Zn translocation in Zn-deficient rice plants (2008)
  • Author(s): Suzuki M, Tsukamoto T, Inoue H, Watanabe S, Matsuha shi S. Takahashi M. Nakanishi H. Mod S. Nishizawa NK
  • Journal Title: Plant Molecular Biology (in press)
  • Peer Reviewed
• [Journal Article] Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants. (2008)
  • Author(s): Ishimam Y, Suzuki M, Ogo Y, Takahashi M, Naka nishi H. Mod S. Nishizawa NK
  • Journal Title: Soil Science and Plant Nutrition (in press)
  • NAID: 110007326872
  • Peer Reviewed
• [Presentation] イネの種子成熟過程における遺伝子発現およびメタボローム解析 (2010)
  • Author(s): 高橋美智子、臼田華奈子、草野都子、及川彰、斉藤和季、平井優美、西澤直子
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] イネの種子成熟過程におけるメタボローム解析 (2009)
  • Author(s): 臼田華奈子、高橋美智子、草野都子、及川彰、斉藤和季、平井優美、西澤直子
  • Organizer: 日本土壌肥料学会2009年度京都大会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-09-17
• [Presentation] イネの種子成熟過程におけるメタボローム解析 (2009)
  • Author(s): 臼田華奈子、高橋美智子、草野都子、及川彰、斉藤和季、平井優美、西澤直子
  • Organizer: 日本士壌肥料学会2009年度京都大会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-09-17
• [Presentation] 生殖成長期および種子成熟期における金属元素動態の解明 (2009)
  • Author(s): 高橋美智子、北島信行、福田直樹、長坂征治、保倉明子、寺田靖子、中井泉、西澤直子
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 2009-03-21
• [Presentation] Metal Distribution in Rice Seeds during The Germination Process using X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu. (2009)
  • Author(s): Michiko Takahashi, Nobuyuki Kitajima, Naoki Fukuda, Akiko Hokura, Yasuko Terada, Izumi Nakai, Naoko K Nishizawa.
  • Organizer: International Symposium on Metallomics Conference
  • Place of Presentation: シンシナティU.S.A.
• [Presentation] In vivo analysis of metal distribution in rice seeds during the germination process using X-ray fluorescence imaging of Fe, Zn, Mn, and Cu. (2009)
  • Author(s): Michiko Takahashi, Tomoko Nozoye, Nobuyuki Kitajima, Naoki Fukuda, Akiko Hokura, Yasuko Terada, Izumi Nakai, Naoko K Nishizawa.
  • Organizer: The International Plant Nutrition Colloquium.
  • Place of Presentation: Sacramento, California U.S.A.
• [Presentation] Metal Distribution in Rice Seeds during The Germination Process using X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu (2009)
  • Author(s): Michiko Takahashi, Nobuyuki Kitajima, Naoki Fukuda, Akiko Hokura, Yasuko Terada, Izumi Nakai, Naoko K Nishizawa
  • Organizer: International Symposium on Metallomics Conference
  • Place of Presentation: シンシナティ U.S.A.
• [Presentation] In vivo analysis of metal distribution in rice seeds during the germination process using X-ray fluorescence imaging of Fe, Zn, Mn, and Cu (2009)
  • Author(s): Michiko Takahashi, Tomoko Nozoye, Nobuyuki Kitajima, Naoki Fukuda, Akiko Hokura, Yasuko Terada, Izumi Nakai, Naoko K Nishizawa
  • Organizer: The International Plant Nutrition Colloquium
  • Place of Presentation: Sacramento, California U.S.A.
• [Presentation] 種子発芽過程における金属移行 (2008)
  • Author(s): 高橋美智子、北島信行、福田直樹、保倉明子、寺田靖子、中井泉、西澤直子
  • Organizer: 日本土壌肥料学会2008年大会
  • Place of Presentation: 名古屋市立大学畑キャンパス川澄キャンパス
  • Year and Date: 2008-09-10
• [Remarks]
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/PNP/