New insights from archaea into researches on isoprenoid metabolism

• Project/Area Number: 20780053
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Nagoya University
• Principal Investigator: HEMMI Hisashi Nagoya University, 大学院・生命農学研究科, 准教授 (60302189)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: アーキア / イソプレノイド / 膜脂質 / 糖キャリア脂質 / メタノフェナジン / ゲラニルゲラニル基還元酵素 / イソペンテニル二リン酸イソメラーゼ / ゲラニルファルネシル二リン酸合成酵素 / プレニル二リン酸合成酵素 / 還元酵素 / 異性化酵素

Research Abstract

Archaea produces many isoprenoid compounds that have structures distinct from those of bacterial or eukaryotic analogues. We isolated novel enzymes involved in their biosynthesis and made characterization or mechanistic analysis of the isoprenoid biosynthetic enzymes, which led us to further understanding of isoprenoid metabolism in archaea and gave us a knowledge of the evolution of the metabolic pathway.

Research Products

• [Journal Article] Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution. (2010)
  • Author(s): Ogawa, T., Yoshimura, T., Hemmi, H.
  • Journal Title: Biochemical and Biophysical Research Communications vol.393 Pages: 16-20
  • Peer Reviewed
• [Journal Article] Geranylfarnesyl diphosphate synthase from Methanosarcina mazei Different role, different evolution (2010)
  • Author(s): Ogawa T., et al.
  • Journal Title: Biochemical and Biophysical Research Communications 393 Pages: 16-20
  • Peer Reviewed
• [Journal Article] Effect of mutagenesis at the region upstream from the G(Q/E) motif of three types of geranylgeranyl diphosphate synthase on product chain-length. (2009)
  • Author(s): Noike, M., Katagiri, T., Nakayama, T., Nishino, T., Hemmi, H.
  • Journal Title: Journal of Bioscience and Bioengineering vol.107 Pages: 235-239
  • Peer Reviewed
• [Journal Article] Flavin' s new role as a general acid-base catalyst with no redox function in type 2 isopentenyl diphosphate isomerase. (2009)
  • Author(s): Unno, H., Yamashita, S., Ikeda, Y., Sekiguchi, S., Yoshida, N., Yoshimura, T., Kusunoki, M., Nakayama, T., Nishino, T., Hemmi, H.
  • Journal Title: Journal of Biological Chemistry vol.284 Pages: 9160-9167
  • Peer Reviewed
• [Journal Article] Effect of mutagenesis at the region upstream from the G(Q/E) motif of three types of geranylgeranyl diphosphate synthase on (2009)
  • Author(s): Noike M., et al.
  • Journal Title: Journal of Bioscience and Bioengineering 107 Pages: 235-239
  • Peer Reviewed
• [Journal Article] New role of flavin as a general acid-base catalyst with no redox function in type 2 isopentenyl… (2009)
  • Author(s): Unno H., et al.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 9160-9167
  • Peer Reviewed
• [Presentation] メタン生成アーキアMethanosarcina mazei由来ゲラニルファルネシルニリン酸合成酵素 (2010)
  • Author(s): 小川拓哉、吉村徹、邊見久
  • Organizer: 2010年度日本農芸化学会大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-28
• [Presentation] メタン生成アーキアMethanosarcina mazei由来ゲラニルファルネシルニリン酸合成酵素 (2010)
  • Author(s): 小川拓哉, 他
  • Organizer: 2010年度日本農芸化学会大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-28
• [Presentation] Type 2 isopentenyl diphosphate isomerase: unusual reaction mechanism of a flavoenzyme (2009)
  • Author(s): Hisashi Hemmi, Hideaki Unno, Satoshi Yamashita, Yosuke Ikeda, Shin-ya Sekiguchi, Norie Yoshida, Tohru Yoshimura, Masami Kusunoki, Toru Nakayama, Tokuzo Nishino
  • Organizer: Italy-Japan Symposium of New Trends in Enzyme Science and Technology
  • Place of Presentation: Institute of Protein Biochemistry, Naples
  • Year and Date: 2009-10-26
• [Presentation] Type 2 isopentenyl diphosphate isomerase : unusual reaction mechanism of a flavoenzyme (2009)
  • Author(s): Hisashi Hemmi, et al.
  • Organizer: Italy-Japan Symposium of New Trends in Enzyme Science and Technology
  • Place of Presentation: Institute of Protein Biochemistry, Naples
  • Year and Date: 2009-10-26
• [Presentation] アーキア膜脂質の生合成におけるゲラニルゲラニル基還元酵素の役割 (2009)
  • Author(s): 邊見久
  • Organizer: 第61回日本生物工学会大会シンポジウム「脂質工学の将来像」
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-25
• [Presentation] アーキア膜脂質の生合成におけるゲラニルゲラニル基還元酵素の役割 (2009)
  • Author(s): 邊見久
  • Organizer: 第61回日本生物工学会大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-25
• [Presentation] 好熱性アーキアSulfolobus acidocaldarius由来ゲラニルゲラニルレダクターゼの酵素学的研究 (2009)
  • Author(s): 岩田有希、吉村徹、邊見久
  • Organizer: 日本Archaea研究会第22回講演会
  • Place of Presentation: 産業技術総合研究所北海道センター
  • Year and Date: 2009-07-10
• [Presentation] 古細菌膜脂質生合成に関わるフラビン酵素の分子機構解明と応用 (2009)
  • Author(s): 邊見久
  • Organizer: 第10回酵素応用シンポジウム
  • Place of Presentation: メルパルクNAGOYA
  • Year and Date: 2009-06-12
• [Presentation] 古細菌膜脂質生合成に関わるフラビン酵素の分子機構解明と応用 (2009)
  • Author(s): 邊見久
  • Organizer: 第10回酵素応用シンポジウム
  • Place of Presentation: メルパルク名古屋
  • Year and Date: 2009-06-12
• [Presentation] 部位特異的変異導入によるタイプ2イソペンテニル二リン酸イソメラーゼの反応機構解析 (2009)
  • Author(s): 遊見久、長井拓也、海野英昭、吉村徹
  • Organizer: 日本ビタミン学会第61回大会
  • Place of Presentation: 京都学園大学
  • Year and Date: 2009-05-31
• [Presentation] The reaction mechanism of type 2 isopentenyl diphosphate isomerase (2009)
  • Author(s): Hisashi Hemmi, Hideaki Unno, Satoshi Ya-ashita, Yosuke Ikeda, Shin-ya Sekiguchi, Norie Yoshida, Tohru Yoshirnura, Masami Kusunoki, Toru Nakayama, Tokuzo Nishino
  • Organizer: TERPNET 2009 9th Intemational Meeting: Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites
  • Place of Presentation: 東京大学本郷キャンパス弥生講堂
  • Year and Date: 2009-05-28
• [Presentation] The reaction mechanism of type 2 isopentenyl diphosphate isomerase (2009)
  • Author(s): 邊見久, 他
  • Organizer: TERPNET 2009
  • Place of Presentation: 東京大学
  • Year and Date: 2009-05-28
• [Presentation] The role of Gln160 in the reaction of type 2 isopentenyl diphosphate isomerase (2009)
  • Author(s): Takuya Nagai, et al.
  • Organizer: TERPNET 2009
  • Place of Presentation: 東京大学
  • Year and Date: 2009-05-28
• [Presentation] TERPNET 2009 9th International Meeting: Biosynthesis and Function of Isoprenoids in Plants (2009)
  • Author(s): Takuya Nagai, Tohru Yoshimura, Hisashi Hemmi
  • Organizer: TERPNET 2009 9th International Meeting: Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites
  • Place of Presentation: 東京大学本郷キャンパス弥生講堂
  • Year and Date: 2009-05-27
• [Presentation] タイプ2イソペンテニルニリン酸イソメラーゼの反応機構:一般酸塩基触媒としてのフラビンの機能 (2009)
  • Author(s): 邊見久、海野英昭、山下哲、池田陽介、関口信哉、吉田宜永、吉村徹、楠木正巳、中山亨、西野徳三
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-03-29
• [Presentation] タイプ2イソペンテニル二リン酸イソメラーゼの反応機構:一般酸塩基触媒としてのフラビンの機能 (2009)
  • Author(s): 邊見久, 他
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-03-29
• [Presentation] タイプ2イソペンテニルニリン酸イソメラーゼの反応機構 (2008)
  • Author(s): 邊見久、海野英昭、山下哲、池田陽介、関口信哉、吉田宜永、中山亨、西野徳三、楠木正巳、吉村徹
  • Organizer: 第18回ドリコールおよびイソプレノイド研究会例会
  • Place of Presentation: 東北大片平さくらホール
  • Year and Date: 2008-08-30
• [Presentation] タイプ2イソペンテニル二リン酸イソメラーゼの反応機構 (2008)
  • Author(s): 邊見久, 他
  • Organizer: 第18回ドリコールおよびイソプレノイド研究会例会
  • Place of Presentation: 東北大学
  • Year and Date: 2008-08-30
• [Presentation] タイプ2イソペンテニル二リン酸イソメラーゼの結晶構造と予想される反応機構 (2008)
  • Author(s): 邊見久、海野英昭、池田陽介、関口信哉、吉田宜永、中山亨、西野徳三、楠木正巳、吉村徹
  • Organizer: 第60回日本生物工学会大会
  • Place of Presentation: 東北学院大学
  • Year and Date: 2008-08-27
• [Presentation] 好熱好酸性アーキアの糖キャリア脂質 (2008)
  • Author(s): 宮川真仁、高橋義洋、吉村徹、西野徳三、邊見久
  • Organizer: 日本Archaea研究会第21回講演会
  • Place of Presentation: 沖縄県男女共同参画センター「ているる」
  • Year and Date: 2008-07-06
• [Presentation] 好熱好酸性アーキアの糖キャリア脂質 (2008)
  • Author(s): 宮川真仁, 他
  • Organizer: 日本Archaea研究会第21回講演会
  • Place of Presentation: 那覇てぃるる
  • Year and Date: 2008-07-06
• [Presentation] Geranylgeranyl Reductases Responsible for Archaeal Membrane Lipid Biosynthesis (2008)
  • Author(s): Hisashi Hemmi, Motomichi Murakami, Sho Sato, Kyohei Shibuya, Yoshihiro Takahashi, Toru Nakayama, Tokuzo Nishino, Tohru Yoshimura
  • Organizer: 7th US-Japan Seminar on the Biosynthesis of Natural Products: Enzymology, Structural Biology, and Drug Discovery
  • Place of Presentation: カリフォルニア大サンディエゴ校
  • Year and Date: 2008-06-24
• [Presentation] Geranylgeranyl Reductases Responsible for Archaeal Membrane Lipid Biosynthesis (2008)
  • Author(s): 邊見久, 他
  • Organizer: 7^<th> US-Japan Seminar on the Biosynthesis of Natural Products
  • Place of Presentation: 米国カリフォルニア大学サンディエゴ校
  • Year and Date: 2008-06-24
• [Presentation] 好熱性アーキア由来タイプ2イソペンテニル二リン酸イソメラーゼの結晶構造解析 (2008)
  • Author(s): 邊見久、海野英昭、池田陽介、関口信哉、吉田宜永、中山亨、西野徳三、楠木正巳、吉村徹
  • Organizer: 日本ビタミン学会第60回大会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2008-06-13
• [Book] "Mevalonate Pathway in Bacteria and Archaea" In Comprehensive Natural Products II Chemistry and Biology (2010)
  • Author(s): Kuzuyama T, Hemmi, H, Takahashi S.
  • Total Pages: 24
  • Publisher: Elsevier