Symbiotic relationship between human and bifidobacteria : Understanding and exploiting the functions of bifidobacterial glycosidases.

• Project/Area Number: 20780056
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: KATAYAMA Takane Ishikawa Prefectural University, 生物資源環境学部, 准教授 (70346104)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ビフィズス菌 / ヒトミルクオリゴ糖 / ムチン型糖鎖 / グリコシダーゼ / フコシダーゼ / フコシルオリゴ糖 / グリコシンターゼ / エンド-α-N-アセチルガラクトサミニダーゼ / Bifidobacterium bifidum / Bifidobacterium longum / lacto-N-biosidase / lacto-N-biose transporter / human milk oligosaccharide

Research Abstract

Breast-fed infants normally have intestinal microbiota dominated by bifidobacteria, in contrast to formula-fed infants. This selective growth has been attributed to oligosaccharides contained in human milk (HMOs). We found that several bifidobacterial strains have a unique metabolic pathway for degrading HMOs. We isolated the specific enzymes involved in this pathway, determined their properties and x-ray structures, and revealed their structure-function relationship. Also, we succeeded in synthesizing 2'-fucosyllactose, a major component of HMOs, by exploiting 1, 2-α-L-fucosidase.

Research Products

• [Journal Article] Analyses of bifidobacterial glycosidases involved in the metabolism of oligosaccharides. (2010)
  • Author(s): Yamamoto, K., Katayama, T., Kitaoka, M., Fushinobu, S.
  • Journal Title: Bioscience and Microflora 29(1) Pages: 23-30
  • NAID: 10026975142
  • Peer Reviewed
• [Journal Article] Analyses of bifidobacterial glycosidases involved in the metabolism of oligosaccharides (2010)
  • Author(s): Yamamoto, Katayama, Kitaoka, Fushinobu
  • Journal Title: Bioscience and Microflora 29 Pages: 23-30
  • NAID: 10026975142
  • Peer Reviewed
• [Journal Article] Crystal structure of GH101 endo-α-N-acetylgalactosaminidase from Bifidobacterium longum. (2009)
  • Author(s): Suzuki, R., Katayama, T., Fushinobu, S., Kitaoka, M., Kumagai, H., Wakagi, T., Shoun, H., Ashida, H., Yamamoto, K.
  • Journal Title: J. Appl. Glycosci. 56 Pages: 105-110
  • NAID: 10025409021
  • Peer Reviewed
• [Journal Article] Conversion of inverting glycoside hydrolases into catalysts for synthesizing glycosides employing a glycosynthase technology. (2009)
  • Author(s): Kitaoka, M., Honda, Y., Fushinobu, S., Hidaka, M., Katayama, T., Yamamoto, K.
  • Journal Title: Trends in Glycoscience and Glycotechnology. 21(117) Pages: 23-39
  • Peer Reviewed
• [Journal Article] Two distinctα-L-fucosidases from Bifidobacterium bifidum are essential for the utilization of fucosylated milk oligosaccharides and glycoconjugates. (2009)
  • Author(s): Ashida, H., Miyake, A., Kiyohara, M., Wada, J., Yoshida, E., Kumagai, H., Katayama, T., Yamamoto, K
  • Journal Title: Glycobiology 19(9) Pages: 1010-1017
• [Journal Article] Crystallographic and mutational analyses of substrate recognition of endo-α-N-acetylgalactosaminidase from Bifidobacterium longum. (2009)
  • Author(s): Suzuki, R., Katayama, T., Kitaoka, M., Kumagai, H., Wakagi, T., Shoun, H., Ashida, H., Yamamoto, K. Fushinobu, S.
  • Journal Title: J. Biochem. 146(3) Pages: 389-398
  • NAID: 10026303375
• [Journal Article] Two distinct α-L-fuxosidases from Bifidobacterium bifidum are essential for the utilization of fucosylated milk oligosaccharides and glycoconjugates (2009)
  • Author(s): Ashida, Miyake, Kiyohara, Wada, Yoshida, Kumagai, Katayama, Yamamoto
  • Journal Title: Glycobiology 19 Pages: 1010-1017
  • Peer Reviewed
• [Journal Article] Crystallographic and mutational analyses of substrate recognition of endo-α-N-acetylgalactosaminidase from Bifidobacterium longum (2009)
  • Author(s): Suzuki, Katayama, Kitaoka, Kumagai, Wakagi, Shoun, Ashida, Yamamoto, Fushinobu
  • Journal Title: Journal of Biochemistry 146 Pages: 389-398
  • NAID: 10026303375
  • Peer Reviewed
• [Journal Article] Functions of novel glycosidases isolated from bifidobacteria. (2008)
  • Author(s): Katayama, T., Wada, J., Fujita, K., Kiyohara, M., Ashida, H., Yamamoto, K.
  • Journal Title: J. Appl. Glycosci. 55 Pages: 101-109
  • NAID: 10020738602
• [Journal Article] 1, 2-α-L-Fucosynthase: A glycosynthse derived from an invertingα-glycosidase with an unusual reaction mechanism. (2008)
  • Author(s): Wada, J., Honda, Y., Nagae, M., Kato, R., Wakatsuki, S., Katayama, T., Taniguchi, H., Kumagai, H., Kitaoka, M., Yamamoto, K.
  • Journal Title: FEBS Lett. 582 Pages: 3739-3743
• [Journal Article] Bifidobacterium bifidum lacto-N-biosidase, a critical enzyme for the degradation of human milk oligosaccharides with a type-1 structure. (2008)
  • Author(s): Wada, J., Ando, T., Kiyohara, M., Ashida, H., Kitaoka, M., Yamaguchi, M., Kumagai, H., Katayama, T., Yamamoto, K.
  • Journal Title: Appl. Environ. Microbiol. 74(13) Pages: 3996-4004
• [Journal Article] Structural and thermodynamic analyses of solute-binding protein from Bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I. (2008)
  • Author(s): Suzuki, R., Wada, J., Katayama, T., Fushinobu, S., Wakagi, T., Shoun, H., Sugimoto, H., Tanaka, A., Kumagai, H., Ashida, H., Kitaoka, M., Yamamoto, K.
  • Journal Title: J. Biol. Chem. 283(19) Pages: 13165-13173
• [Journal Article] Structural and thermodynamic analyses of solute-binding protein from Bifidobacterium longum specific for Core 1 disaccharide and lacto-N-biose (2008)
  • Author(s): Ryuichiro Suzuki, et.al.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 13615-16173
  • Peer Reviewed
• [Journal Article] Bifidobacterium bifidum lacto-N-biosidase, a critical enzyme for the degradation of human milk oligosaccharides with a type 1 structure (2008)
  • Author(s): Jun Wada, et.al.
  • Journal Title: Applied and Environmental Microbiology 74 Pages: 3996-4004
  • Peer Reviewed
• [Journal Article] Functions of novel glycosidases isolated from bifidobacteria (2008)
  • Author(s): Takane Katayama, et.al.
  • Journal Title: Journal of Applied Glycosciences 55 Pages: 101-109
  • NAID: 10020738602
• [Presentation] ビフィズス菌由来1, 2-α-L-フコシダーゼを用いたヒトミルクオリゴ糖2'-フコシルラクトースの合成 (2009)
  • Author(s): 片山高嶺、和田潤、本多裕司、長江雅倫、加藤龍一、若槻壮市、谷口肇、熊谷英彦、北本光、山本憲二
  • Organizer: 2009年度乳酸菌・腸内細菌工学研究部会講演会
  • Place of Presentation: 加賀観光ホテル
  • Year and Date: 2009-05-15
• [Presentation] ビフィズス菌由来1,2-α-L-フコシダーゼを用いたヒトミルクオリゴ糖2-フコシルラクトースの合成 (2009)
  • Author(s): 片山高嶺、和田潤、本多裕司、長江雅倫、加藤龍一、若槻壮市、谷口肇、熊谷英彦、北岡本光、山本憲二
  • Organizer: 2009年度乳酸菌・腸内細菌工学研究部会講演会
  • Place of Presentation: 加賀観光ホテル
  • Year and Date: 2009-05-15
• [Presentation] Bifidobacterium bifidumが分泌する特異的なα-1, 3/4-L-fucosidaseの解析 (2009)
  • Author(s): 三宅章子、清原正志、和田潤、片山高嶺、芦田久、山本憲二
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2009-03-28
• [Presentation] A unique pathway in bifidobacteria for the degradation of human milk oligosaccharides (2008)
  • Author(s): Takane Katayama
  • Organizer: The 11^<th> Swiss-Japanese Joint Meeting on Biotechnology and Bioprocess Development
  • Place of Presentation: Minusio, Switzerland
  • Year and Date: 2008-10-28
• [Presentation] ビフィズス菌のヒトミルクオリゴ糖資化経路 (2008)
  • Author(s): 和田潤、清原正志、片山高嶺、芦田久、鈴木龍一郎、伏信進矢、熊谷英彦、北岡本光、山本憲二
  • Organizer: 2008年日本糖質学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-08-19
• [Presentation] ビフィズス菌のヒトミルクオリゴ糖資化経路 (2008)
  • Author(s): 和田潤
  • Organizer: 日本糖質学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-08-19
• [Presentation] ヒトミルクオリゴ糖に作用するビフィズス菌のグリコシダーゼ (2008)
  • Author(s): 片山高嶺、和田潤、山本憲二、芦田久、清原正志
  • Organizer: 2008年度日本乳酸菌学会
  • Place of Presentation: 京都大学百周年時計台記念館
  • Year and Date: 2008-07-15
• [Presentation] ヒトミルクオリゴ糖に作用するビフィズス菌のグリコシダーゼ (2008)
  • Author(s): 片山高嶺
  • Organizer: 日本乳酸菌学会
  • Place of Presentation: 京都大学百周年時計台記念館
  • Year and Date: 2008-07-15