| Project/Area Number |
20780057
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
HAMANO Yoshimitsu Fukui Prefectural University, 生物資源学部, 講師 (50372834)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アミノ酸ホモポリマー / ポリアミド / 非リボソームペプチド合成酵素 / ペプチド / 放線菌 / ポリリジン / Streptomyces albulus / 二次代謝産物 / 生合成遺伝子 / 抗生物質 / NRPS / Stretomyces albulus |
|---|
| Research Abstract |
epsilon-Poly-L-Lysine (ε-PL) produced by microorganisms is a highly safe antibacterial substance used widely as one of the world's few natural food preservatives. Although ε-PL has a simple polymer structure consisting of a normal chain of lysine, an amino acid, how it is synthesized inside microorganisms had not been clarified. In this study, we characterized the ε-PL synthetase and its gene. We also revealed that the ε-PL synthetase has a highly unusual domain architecture and reaction mechanism (Nature Chemical Biology, 4, 766-772, 2008).
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