| Project/Area Number |
20780058
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
TANI Shuji Osaka Prefecture University, 生命環境科学研究科, 助教 (80405357)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Aspergillus aculeatus / (hemi)cellulase / gene regulation / Agrobacterium tumefaciens-mediated transformation / TAIL-PCR / Insertional mutagenesis / Agrobacterium tumefaciens / Transformation / Gene tagging / Cellulase / Gene regulation / Agrobacterium / transformation |
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| Research Abstract |
We report the successful Agrobacterium tumefaciens-mediated transformation (AMT) of Aspergillus aculeatus for functional genomics. We isolated 40 transformants expected to be disruptants of the regulator(s) controlling cellulase gene expressions in A. aculeatus from 15,000 transformants obtained by AMT. Mutation of the geneA resulted in 5-fold reduction on cellobiohydrolase gene expression in transformant by comparing with that in host strain. Now, we are further analyzing the function of putative transcription factor identified in this study and seeking the other regulators.
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