Evaluation of oxygen sensing mechanism(s) in lactic acid bacterial
| Project/Area Number |
20780060
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kitasato University |
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Principal Investigator |
YAMAMOTO Yuji Kitasato University, 獣医学部, 講師 (10453507)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 微生物制御 / 発現制御 / 乳酸菌 / 酸素 / 活性酸素 / 連鎖球菌 / 制御メカニズム / 応答 |
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| Research Abstract |
In this study we investigated the oxygen sensing mechanism(s) in lactic acid bacteria, and identified Spx homologues (SpxA and SpxB), which are known as RNA-binding protein of Bacillus subtilis, as regulators required for oxygen-dependent induction of antioxidants in Streptococcus mutans. The spxA and spxB defective mutant of S. mutans was shown to lose the abilities to induce the expression of antioxidants and to grow under aerobic conditions. We also purified recombinant SpxA and SpxB proteins and constructed an assay system to distinguish oxidized- and reduced-form of the proteins.
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Report
(3 results)
Research Products
(13 results)
