| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In this study we investigated the oxygen sensing mechanism(s) in lactic acid bacteria, and identified Spx homologues (SpxA and SpxB), which are known as RNA-binding protein of Bacillus subtilis, as regulators required for oxygen-dependent induction of antioxidants in Streptococcus mutans. The spxA and spxB defective mutant of S. mutans was shown to lose the abilities to induce the expression of antioxidants and to grow under aerobic conditions. We also purified recombinant SpxA and SpxB proteins and constructed an assay system to distinguish oxidized- and reduced-form of the proteins.
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