| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
The genes involving archaeosine biosynthesis were successfully disrupted, indicating that these genes are not essential for cell viability. The growth profiles of ΔqueD, Δtgt and ΔarcS mutants were almost identical with that of host strain at 85℃ (the optimum growth temperature) and 93℃ (the upper limit of growth temperature), suggesting that archaeosine could not contribute to stabilization the tertiary structure of tRNA. The queE gene disruptant showed poor cell growth at 85 and 93℃. The queC gene disruptant showed poor cell growth at 93℃.
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