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Elucidation of archaea-specific archaeosine modification system

Research Project

Project/Area Number 20780062
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionToyo University

Principal Investigator

HIGASHIBATA Hiroki  東洋大学, 生命科学部, 准教授 (20344864)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords微生物代謝 / アーキア / tRNA / tRNA修飾酵素
Research Abstract

The genes involving archaeosine biosynthesis were successfully disrupted, indicating that these genes are not essential for cell viability. The growth profiles of ΔqueD, Δtgt and ΔarcS mutants were almost identical with that of host strain at 85℃ (the optimum growth temperature) and 93℃ (the upper limit of growth temperature), suggesting that archaeosine could not contribute to stabilization the tertiary structure of tRNA. The queE gene disruptant showed poor cell growth at 85 and 93℃. The queC gene disruptant showed poor cell growth at 93℃.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (20 results)

All 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (16 results) Remarks (2 results)

  • [Journal Article] アーキアの核酸安定化戦略2010

    • Author(s)
      東端啓貴
    • Journal Title

      極限環境微生物学会誌

      Volume: 8巻2号 Pages: 69-76

    • NAID

      130004486998

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] アーキアの核酸安定化戦略2010

    • Author(s)
      東端啓貴
    • Journal Title

      極限環境微生物学会誌 8(2)

      Pages: 69-76

    • NAID

      130004486998

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Thermococcus kodakarensisのアーキオシン生合成関連遺伝子破壊株の増殖特性2011

    • Author(s)
      東端啓貴, 他2名
    • Organizer
      第12回極限環境生物学会年会
    • Place of Presentation
      良順会館(長崎大学)
    • Year and Date
      2011-11-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Thermococcus kodakarensisのアーキオシン生合成関連遺伝子破壊株の増殖特性2011

    • Author(s)
      東端啓貴
    • Organizer
      第12回極限環境生物学会年会
    • Place of Presentation
      長崎大学良順会館(長崎県)
    • Year and Date
      2011-11-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Disruption of genes involved in archaeosine biosynthesis in hyperthermophilic archaeon Thermococcus kodakarensis2011

    • Author(s)
      Hiroki, HIGASHIBATA, 他1名
    • Organizer
      Thermophiles2011
    • Place of Presentation
      Huntley Lodge Bigsky, Montana, America
    • Year and Date
      2011-09-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Disruption of genes involved in archaeosine biosynthesis in hyperthermophilic archaeon Thermococcus kodakarensis2011

    • Author(s)
      Hiroki, HIGASHIBATA
    • Organizer
      Thermophiles 2011
    • Place of Presentation
      Huntley Lodge Bigsky, Montana, America
    • Year and Date
      2011-09-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] アーキオシン生合成遺伝子破壊株の増殖特性2011

    • Author(s)
      東端啓貴, 他2名
    • Organizer
      日本農芸化学会2011年度(平成23年度)大会(震災により中止したが、要旨集の発行を以て開催したとみなす)
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Final Research Report
  • [Presentation] アーキオシン生合成遺伝子破壊株の増殖特性2011

    • Author(s)
      東端啓貴
    • Organizer
      日本農芸化学会2011年度(平成23年度)大会
    • Place of Presentation
      学会開催予定地:京都女子大学(京都府)(震災により中止となったが、大会講演要旨集の発行をもって成立とする)
    • Year and Date
      2011-03-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Disruption of genes involved in archaeosine biosynthetic pathway in hyperthermophilic archaeon Thermococcus kodakarensis2010

    • Author(s)
      Hiroki, HIGASHIBATA, 他1名
    • Organizer
      Pacifichem2010
    • Place of Presentation
      Hawai'i Convention Center, Honolulu, Hawaii, America
    • Year and Date
      2010-12-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Disruption of genes involved in archaeosine biosynthetic pathway in hyperthermophilic archaeon Thermococcus kodakarensis2010

    • Author(s)
      Hiroki, HIGASHIBATA
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Hawai'i Convention Center, Honolulu, Hawaii, America
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超好熱性アーキアThermococcus kodakarensisのアーキオシン生合成経路2010

    • Author(s)
      東端啓貴, 他1名
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 超好熱性アーキアThermococcus kodakarensisのアーキオシン生合成経路2010

    • Author(s)
      東端啓貴
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超好熱菌由来のアーキオシン生合成遺伝子の解析2010

    • Author(s)
      馬渡麻未、東端啓貴, 他1名
    • Organizer
      第11回極限環境生物学会年会
    • Place of Presentation
      京都大学化学研究所(京都)
    • Year and Date
      2010-11-16
    • Related Report
      2010 Final Research Report
  • [Presentation] 超好熱菌由来のアーキオシン生合成遺伝子の解析2010

    • Author(s)
      馬渡麻未、東端啓貴, 他1名
    • Organizer
      第11回極限環境生物学会年会
    • Place of Presentation
      京都大学化学研究所(京都府)
    • Year and Date
      2010-11-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Archaeosine biosynthetic pathway in hyperthermophilic archaeon Thermococcus kodakaraensis2009

    • Author(s)
      東端啓貴
    • Organizer
      東洋大学バイオ・ナノエレクトロニクス研究センター・国際シンポジウム
    • Place of Presentation
      東洋大学(東京)
    • Year and Date
      2009-11-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Archaeosine biosynthetic pathway in hyper the rmophilic archaeon The rmococcus kodakaraensis2009

    • Author(s)
      東端啓貴
    • Organizer
      東洋大学バイオ・ナノエレクトロニクス研究センター・国際シンポジウム
    • Place of Presentation
      東洋大学(東京)
    • Year and Date
      2009-11-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] アーキオシン生合成遺伝子の解析2009

    • Author(s)
      東端啓貴
    • Organizer
      第10回極限環境微生物学会年会
    • Place of Presentation
      明治大学(東京)
    • Year and Date
      2009-10-29
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 超好熱性アーキアの核酸関連酵素の解析2009

    • Author(s)
      東端啓貴
    • Organizer
      極限環境微生物学会第10回シンポジウム
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2009-06-09
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Remarks]

    • URL

      http://ris.toyo.ac.jp/details/index.php?user_id=790

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://ris.toyo.ac.jp/details/index.php?user_id=790

    • Related Report
      2010 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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