Analysis and application of the availability of an inorganic polyphosphate specific to Mycobacterium tuberculosis

• Project/Area Number: 20780066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MORI Shigetarou 国立感染症研究所, 細菌第二部, 主任研究官 (60425676)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 結核菌 / ヌクレオチド / 加リン酸分解 / 遺伝子 / タンパク質 / 立体構造解析 / 加リン酸分解酵素

Research Abstract

In this study, the function and structure of a polyphosphate-related protein-Rv2613c-was analyzed. This protein is encoded by Rv2613c, a gene that is found in Mycobacterium tuberculosis H37Rv and has unknown function. The results showed that Rv2613c has diadenosine polyphosphate(Ap_nA) phosphorylase activity. Furthermore, Rv2613c was found to have a tetrameric structure that is unique among enzymes that use Ap_nA as a substrate, and it was found that the formation of the tetrameric structure of Rv2613c is essential for its enzymatic activity.

Research Products

• [Journal Article] Structural insights into the novel diadenosine 5', 5'_P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2011)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Journal Title: Journal of Molecular Biology Volume: 410 Issue: 1 Pages: 93-104
  • DOI: 10.1016/j.jmb.2011.04.059
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Journal Title: Acta Crystallographica Section F Volume: 66 Issue: 3 Pages: 279-281
  • DOI: 10.1107/s174430910905444x
  • Peer Reviewed
• [Journal Article] Purification and molecular characterization of a novel diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Journal Title: Protein Expression and Purification Volume: 69 Issue: 1 Pages: 99-105
  • DOI: 10.1016/j.pep.2009.09.010
  • Peer Reviewed
• [Journal Article] Crystallographic analysis of 5', 5"'-P^1,P^4-tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、荒川宜親
  • Journal Title: Photon Factory Activity Report 27巻 Pages: 228-228
• [Journal Article] Crystallization andpreliminary X-ray analysis of the diadenosine 5', 5"'-P^1,P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosisH37Rv (2010)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、荒川宜親
  • Journal Title: Acta Crystallogr. F66巻 Pages: 279-281
  • Peer Reviewed
• [Journal Article] Purification and molecular characterization of a novel diadenosine 5', 5"'-P^1,P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、荒川宜親
  • Journal Title: Protein Expr. Purif. 69巻 Pages: 99-105
  • Peer Reviewed
• [Journal Article] Purification and molecular characterization of a novel diadenosine 5',5"'-P(1),P(4)-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): 森茂太郎
  • Journal Title: Protein Expression and Purification 69 Pages: 99-105
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the diadenosine 5',5"'-P1,P4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): 森茂太郎
  • Journal Title: Acta Crystallographica Section F 66 Pages: 279-281
  • Peer Reviewed
• [Journal Article] Structural Insights into the Novel Diadenosine 5',5"'-P^1,P^4-Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv
  • Author(s): 雄郎、柴山恵吾、和知野純一、荒川宜親
  • Journal Title: Journal of Molecular Biology in Press
  • Peer Reviewed
• [Presentation] 結核菌由来ニコチン酸ホスホリボシルトランスフェラーゼの機能解析とピラジン酸による抗結核菌活性との関連性 (2012)
  • Author(s): 森茂太郎
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都府京都市)
  • Year and Date: 2012-03-24
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の活性発現に関わる構造的要因の解明 (2011)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 第63回日本生物工学大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2011-09-26
• [Presentation] 結核菌由来新規diadenosine 5',5"'-P^1,P^4-tetraphosphate加リン酸分解酵素の活性発現に関わる構造的要因の解明 (2011)
  • Author(s): 森茂太郎
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大学(東京都小金井市)
  • Year and Date: 2011-09-26
• [Presentation] Structural Insights into the Novel Diadenosine Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv (2011)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-10
• [Presentation] Structural Insights into the Novel Diadenosine Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv (2011)
  • Author(s): Shigetarou Mori
  • Organizer: International Union of Microbiological Societies 2011 congress
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
  • Year and Date: 2011-09-10
• [Presentation] 結核菌由来新規diadenosine 5',5'''-P^1,P^4-tetraphosphate加リン酸分解酵素の活性発現に必要な構造的要因 (2011)
  • Author(s): 森茂太郎
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: (要旨集発行にて大会成立(京都府京都市で開催予定))
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の機能構造相関解析 (2010)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎シーガイア(宮崎県)
  • Year and Date: 2010-10-29
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の構造と機能の相関解析 (2010)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 第93回日本細菌学会関東支部総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-10-21
• [Presentation] Cloning, purification, and molecular characterization of a novel diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Organizer: The 110^<th> General Meeting of the American Society for Microbiology
  • Place of Presentation: San Dieeo Convention Center(USA)
  • Year and Date: 2010-05-27
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の結晶構造解析 (2010)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-03-29
• [Presentation] 結核菌由来新規diadenosine 5',5"'-P^1,P^4-tetraphosphate加リン酸分解酵素の機能構造相関解析 (2010)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、荒川宜親
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎市
• [Presentation] Cloning, purification, and molecular characterization of a novel diadenosine 5',5"'-P^1 P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、荒川宜親
  • Organizer: The 110^thGeneral Meeting of the American Society for Microbiology
  • Place of Presentation: San Diego,CA,USA
• [Presentation] 結核菌由来新規diadenosine 5',5"'-P1,p^4-p^1,P^4-tetraphosphate加リン酸分解酵素の結晶構造解析 (2010)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、荒川宜親
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
• [Presentation] Cloning, purification, and molecular characterization of a novel diadenosine 5',5'''-P^1,P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv. (2010)
  • Author(s): 森茂太郎
  • Organizer: The 110^<th> General Meeting of the American Society for Microbiology
  • Place of Presentation: San Diego,CA.アメリカ合衆国
• [Presentation] 結核菌由来新規diadenosine 5',5'''-P^1,P^4-tetraphosphate加リン酸分解酵素の構造と機能の相関解析 (2010)
  • Author(s): 森茂太郎
  • Organizer: 第92回日本細菌学会関東支部総会
  • Place of Presentation: 新宿区(東京医科歯科大学)
• [Presentation] 結核菌由来新規diadenosine 5',5'''-P^1,P^4-tetraphosphate加リン酸分解酵素の機能構造相関解析 (2010)
  • Author(s): 森茂太郎
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎市(宮崎シーガイヤ・ワールドコンベンションセンター)
• [Presentation] 結核菌由来新規diadenosine 5',5"'-P^1,P^4-tetraphosphate加リン酸分解酵素の結晶構造解析 (2010)
  • Author(s): 森茂太郎
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京(東京大学駒場キャンパス)
• [Presentation] 結核菌由来新規ヌクレオチド加水分解酵素の機能解析 (2009)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,朴貞玉,荒川宜親
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2009-03-29
• [Presentation] 結核菌由来新規ヌクレオチド加水分解酵素の機能解析 (2009)
  • Author(s): 森茂太郎
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-03-29
• [Presentation] 結核菌由来の新規ヌクレオチド加水分解酵素に関する研究 (2009)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,朴貞玉,荒川宜親
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-03-13
• [Presentation] 結核菌由来の新規ヌクレオチド加水分解酵素に関する研究 (2009)
  • Author(s): 森茂太郎
  • Organizer: 日本細菌学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-03-13
• [Presentation] 結核菌由来新規ヌクレオチド加水分解酵素の機能解析 (2009)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、朴貞玉、荒川宜親
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] 結核菌由来の新規ヌクレオチド加水分解酵素に関する研究 (2009)
  • Author(s): 森茂太郎、柴山恵吾、和知野純一、朴貞玉、荒川宜親
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Remarks] (1) Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa. Crystallographic analysis of diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv. Photon Factory Activity Report 2009# 27 Par B(2010), P228
• [Remarks] (2) Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa. Crystal structure of a novel diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv. Photon Factory Activity Report 2010# 28 Par B(2011), P260
• [Remarks] (3) Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa. Crystal Structure of a Novel Diadenosine 5', 5'-P^1, P^4-Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv. PDB ID : 3ANO, 2011年5月18日
• [Remarks] 結核菌由来新規Ap_4A加リン酸分解酵素の立体構造についてはProtein Data Bank
  • URL: http://www.pdb.org/