Analysis and application of the availability of an inorganic polyphosphate specific to Mycobacterium tuberculosis
Project/Area Number |
20780066
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
|
Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
MORI Shigetarou 国立感染症研究所, 細菌第二部, 主任研究官 (60425676)
|
Project Period (FY) |
2008 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 結核菌 / ヌクレオチド / 加リン酸分解 / 遺伝子 / タンパク質 / 立体構造解析 / 加リン酸分解酵素 |
Research Abstract |
In this study, the function and structure of a polyphosphate-related protein-Rv2613c-was analyzed. This protein is encoded by Rv2613c, a gene that is found in Mycobacterium tuberculosis H37Rv and has unknown function. The results showed that Rv2613c has diadenosine polyphosphate(Ap_nA) phosphorylase activity. Furthermore, Rv2613c was found to have a tetrameric structure that is unique among enzymes that use Ap_nA as a substrate, and it was found that the formation of the tetrameric structure of Rv2613c is essential for its enzymatic activity.
|
Report
(6 results)
Research Products
(36 results)