Research Project
Grant-in-Aid for Young Scientists (B)
Protein phosphorylation plays a key regulatory role in a variety of cellular processes. To better understand the function of protein phosphorylation in seed maturation, I employed a PCR-based cloning method and isolated five cDNA clones (pvcipk1-5) for protein kinases and two cDNA clones (pvcbl1 and 2) for CBLs from a cDNA library prepared from immature seeds of kidney bean (Phaseolus vulgaris L.). Yeast two-hybrid assay indicated that among the five PvCIPKs, only PvCIPK1 interacts with both PvCBL1 and PvCBL2. These results suggest that calcium-dependent protein phosphorylation-signaling via CBL-CIPK complexes occurs during seed development. Furthermore I identified heat shock protein 82 (Hsp82) and nucleooside diphosphate kinase 1 (NDPK1) as phosphoproteins in rice developing seed. The results of subcellular localization, gene expression and mutant analysis of these proteins indicated that Hsp82 and NDPK1 are involved in starch biosynthesis in plat seed.
All 2009 2008 Other
All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (17 results)
Phytochemistry 70(3)
Pages: 370-379
Phytochemistry 70(4)
Pages: 501-507
Plant J 60(1)
Pages: 146-155
The Plant Journal 60
Phytochemistry 70
Plant J 55(3)
Pages: 443-454
The Plant Journal 55
