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Studies on the mechanism of cell cycle regulation depending on tRNA status

Research Project

Project/Area Number 20780072
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

OGAWA Tetsuhiro  The University of Tokyo, 大学院・農学生命科学研究科, 助教 (40323480)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords微生物 / マイクロアレイ / 発現制御 / tRNA
Research Abstract

To examine the mechanism of cell cycle regulation depending on tRNA status, tRNA knock down was carried out with tRNA-specific RNase, colicin D, in Saccharomyces cerevisiae. Furthermore, Rny1p, which is newly revealed to cleave tRNA resulting in cell cycle arrest during oxdative stress, was expressed in S. cerevisiae, and gene expression profile was comprehensively analyzed by DNA micro array.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (22 results)

All 2010 2009 2008

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (14 results)

  • [Journal Article] Cellular and transcriptional responses of yeast to the cleavage of cytosolic tRNAs induced by colicin2009

    • Author(s)
      茂松恵、小川哲弘、木戸淳裕、北本宏子、日高真誠、正木春彦
    • Journal Title

      D. Yeast 26

      Pages: 663-673

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of D-amino acids in purified proteins synthesized in Escherichia coli.2009

    • Author(s)
      宮本哲也、関根正恵、小川哲弘、日高真誠、本間浩、正木春彦
    • Journal Title

      Amino Acids 38

      Pages: 1377-1385

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 多彩なバクテリオシン:mRNAやtRNAをまねるコリシンの構造と機能2009

    • Author(s)
      正木春彦、小川哲弘、矢嶋俊介
    • Journal Title

      蛋白質核酸酵素 54

      Pages: 593-600

    • Related Report
      2009 Final Research Report
  • [Journal Article] Colicin E5 ribonuclease domain cleaves Saccharomyces cerevisiae tRNAs leading to impairment of the cell growth.2009

    • Author(s)
      小川哲弘、日高真誠、河野憲二、正木春彦
    • Journal Title

      Journal of Biochemistry 145

      Pages: 461-466

    • NAID

      10026302064

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular recognition of a tRNA-specific colicin and its inhibitor protein, both mimicking the mRNA-tRNA interaction2009

    • Author(s)
      正木春彦
    • Journal Title

      蛋白質核酸酵素 54

      Pages: 593-600

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Detection of D : -amino acids in purified proteins synthesized in Escherichia coli2009

    • Author(s)
      宮本哲也
    • Journal Title

      Amino Acids 38

      Pages: 1377-1385

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular and transcriptional responses of yeast to the cleavage of cytosolic tRNAs induced by colicin D2009

    • Author(s)
      茂松恵
    • Journal Title

      Yeast 26

      Pages: 663-673

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Colicin E5 ribonuclease domain cleaves Saccharomyces cerevisiae tRNAs leading to impairment of the cell growth.2009

    • Author(s)
      小川哲弘
    • Journal Title

      Journal of Biochemistry (印刷中)

    • NAID

      10026302064

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] コリシンDの基質tRNAにおける選択性の決定機構2010

    • Author(s)
      小川哲弘、中西孝太郎、伊藤考太郎、高橋一敏、日高真誠、正木春彦
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学駒場キャンパス(東京)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 酸化ストレスに応答したtRNA切断の分子機構の解析2010

    • Author(s)
      大本哲也、茂松恵、小川哲弘、日高真誠、正木春彦
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学駒場キャンパス(東京)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] CELLULAR AND TRANSCRIPTIONAL RESPONSES TO TRNA CLEAVAGE INDUCED BY TRNA-TARGETING RIBONUCLEASE, COLICIN D.2009

    • Author(s)
      茂松恵、大本哲也、小川哲弘、北本宏子、日高真誠、正木春彦
    • Organizer
      Cold Spring Harbor Laboratory Meeting YEAST CELL BIOLOGY
    • Place of Presentation
      Cold Spring Harbor Laboratory (NY, USA)
    • Year and Date
      2009-08-13
    • Related Report
      2009 Final Research Report
  • [Presentation] CELLULAR AND TRANSCRIPTIONAL RESPONSES TO TRNA CLEAVAGE INDUCED BY TRNA-TARGETING RIBONUCLEASE, COLICIN D2009

    • Author(s)
      茂松恵
    • Organizer
      Cold Spring Harbor Laboratory Meeting YEAST CELL BIOLOGY
    • Place of Presentation
      Cold Spring Harbor Laboratory(NY, USA)
    • Year and Date
      2009-08-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] tRNAの切断が接合シグナル伝達系を活性化させる2009

    • Author(s)
      茂松恵、大本哲也、小川哲弘、北本宏子、日高真誠、正木春彦
    • Organizer
      酵母遺伝学フォーラム(つくばノバホール
    • Place of Presentation
      茨城
    • Year and Date
      2009-07-30
    • Related Report
      2009 Final Research Report
  • [Presentation] tRNAの切断が接合シグナル伝達系を活性化させる2009

    • Author(s)
      茂松恵
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      つくばノバホール(茨城県)
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] tRNA損傷による生育阻害を抑制するユビキチンリガーゼSan1p2009

    • Author(s)
      大本哲也、茂松恵、小川哲弘、日高真誠、正木春彦
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      マリンメッセ福岡(福岡)
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] tRNA切断とDNA損傷修復機構との関連性の検討2009

    • Author(s)
      茂松恵、大本哲也、小川哲弘、北本宏子、日高真誠、正木春彦
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      マリンメッセ福岡(福岡)
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] tRNA切断とDNA損傷修復機構との関連性の検討2009

    • Author(s)
      茂松恵
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] tRNA損傷による生育阻害を抑制するユビキチンリガーゼSanlp2009

    • Author(s)
      大本哲也
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] ユビキチンリガーゼがtRNAノックダウンによる細胞周期停止を抑制する2008

    • Author(s)
      大本哲也、茂松恵、小川哲弘、日高真誠、正木春彦
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      北海道大学・学術交流館(北海道)
    • Year and Date
      2008-09-11
    • Related Report
      2009 Final Research Report
  • [Presentation] ユビキチンリガーゼがtRNAノックダウンによる細胞周期停止を抑制する2008

    • Author(s)
      大本哲也
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      北海道大学・学術交流館
    • Year and Date
      2008-09-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] tRNAノックダウンに対するDNA損傷応答の関与2008

    • Author(s)
      茂松恵、大本哲也、小川哲弘、日高真誠、正木春彦
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      北海道大学・学術交流館(北海道)
    • Year and Date
      2008-09-10
    • Related Report
      2009 Final Research Report
  • [Presentation] tRNAノックダウンに対するDNA損傷応答の関与2008

    • Author(s)
      茂松恵
    • Organizer
      酵母遺伝学ラォーラム
    • Place of Presentation
      北海道大学・学術交流館
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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