| Project/Area Number |
20780072
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OGAWA Tetsuhiro The University of Tokyo, 大学院・農学生命科学研究科, 助教 (40323480)
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|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 微生物 / マイクロアレイ / 発現制御 / tRNA |
|---|
| Research Abstract |
To examine the mechanism of cell cycle regulation depending on tRNA status, tRNA knock down was carried out with tRNA-specific RNase, colicin D, in Saccharomyces cerevisiae. Furthermore, Rny1p, which is newly revealed to cleave tRNA resulting in cell cycle arrest during oxdative stress, was expressed in S. cerevisiae, and gene expression profile was comprehensively analyzed by DNA micro array.
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