Environmental factor for cold-adaptation of psychrophilic metalloenzyme

• Project/Area Number: 20780074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kobe University
• Principal Investigator: TSURUTA Hiroki Kobe University, 連携創造本部, 准教授 (20346282)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 低温活性酵素 / 好冷性細菌 / 低温適応 / X線結晶構造解析 / 触媒反応効率 / フォスファターゼ / 低温活性金属酵素

Research Abstract

For the construction of structure leading high cold activity of psychrophilic phosphatase, the magnesium concentration during folding of this enzyme protein was an important environmental factor.

Research Products

• [Journal Article] Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme. (2010)
  • Author(s): M.Nakagawa, M.Ueyama, H.Tsuruta, T.Uno, K.Kanamaru, B.Mikami, H.Yamagata.
  • Journal Title: Journal of Biological Chemistry 285 Pages: 29797-29807
  • NAID: 120003220000
  • Peer Reviewed
• [Journal Article] Tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick, Ixodes persulcatus. (2010)
  • Author(s): E.Isogai, H.Isogai, K.Okumura, H.Hori, H.Tsuruta, Y.Kurebayashi.
  • Journal Title: Experimental & Applied Acarology 53(1) Pages: 71-77
  • Peer Reviewed
• [Journal Article] Crystal structure of cold-active alkaline phosphatase from psychrophile Shewanella sp. (2010)
  • Author(s): H.Tsuruta, B.Mikami, T.Higashi, Y.Aizono.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry 74(1) Pages: 69-74
  • NAID: 10027550057
  • Peer Reviewed
• [Journal Article] Crystal structure of cold-active alkaline phosphatase from psychrophile Shewanella sp. (2010)
  • Author(s): H.Tsuruta, et al.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry 74 Pages: 69-74
  • NAID: 10027550057
  • Peer Reviewed
• [Journal Article] 低温で機能する酵素の機能特性と構造特性、特集「化学品量産プロセスを志向する酵素研究」 (2008)
  • Author(s): 鶴田宏樹、相薗泰生
  • Journal Title: BIO INDUSTRY 25, No.7 Pages: 5-13
  • NAID: 40016106093
• [Journal Article] The Role of Group Bulkiness in the Catalytic Activity of Psychrophile Cold-active Protein Tyrosine Phosphatase (2008)
  • Author(s): H.Tsuruta, B.Mikami, C.Yamamoto, H.Yamagata.
  • Journal Title: FEBS Journal 275 Pages: 4317-4328
  • Peer Reviewed
• [Journal Article] Identification and cloning of a gene encoding tannase (tannin acylhydrolase) from Lactobacillus plantarum ATCC 14917T. (2008)
  • Author(s): K.Iwamoto, H.Tsuruta, Y.Nishitani, R.Osawa.
  • Journal Title: Systematic and Applied Microbiology 31(4) Pages: 269-277
  • NAID: 120001075316
  • Peer Reviewed
• [Journal Article] The Role of Group Bulkiness in the Catalytic Activity of Psychrophile Cold-active Protein Tyrosine Phosphatase (2008)
  • Author(s): H. Tsuruta, et.al.
  • Journal Title: FEBS Journal 275 Pages: 4317-4328
  • Peer Reviewed
• [Journal Article] Identification and cloning of a gene encoding tannase (tannin acylhydrolase) from Lactobacillus plantarum ATCC 14917^T (2008)
  • Author(s): K. Iwamoto, H. Tsuruta, et.al.
  • Journal Title: Systematic and Applied Microbiology 31 Pages: 269-277
  • NAID: 120001075316
  • Peer Reviewed
• [Journal Article] 相菌泰生、低温で機能する酵素の機能特性と構造特性 (2008)
  • Author(s): 鶴田宏樹、相薗泰生
  • Journal Title: BIO INDUSTRY 25 Pages: 5-13
• [Presentation] Structural features for adaptation of psychrophilic cold-active phosphatase (2010)
  • Author(s): H.Tsuruta, B.Mikami.
  • Organizer: PACIFICHEM2010
  • Place of Presentation: Hawaii (USA)
  • Year and Date: 2010-12-18
• [Presentation] Mgイオン存在下で再生したCold-active protein tyrosine phosphataseの立体構造解析 (2010)
  • Author(s): 鶴田宏樹、三上文三
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学、東京
  • Year and Date: 2010-03-28
• [Presentation] Mgイオン存在下で再生したCold-active protein tyrosine phosphataseの立体構造解析 (2010)
  • Author(s): 鶴田宏樹、三上文三
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-03-28
• [Presentation] 好冷菌Shewanella sp.が産生するCold-active alkaline phosphataseの結晶構造 (2009)
  • Author(s): 鶴田宏樹、三上文三、東哲生、相薗泰生
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド、兵庫
  • Year and Date: 2009-10-22
• [Presentation] 好冷菌Shewanella sp.が産生するCold-active alkaline phosphataseの結晶構造 (2009)
  • Author(s): 鶴田宏樹、三上文三、東哲生、相薗泰生
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2009-10-22
• [Presentation] 配位金属の相違が低温活性発現能に与える影響 (2008)
  • Author(s): 鶴田宏樹
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫
  • Year and Date: 2008-12-12
• [Presentation] 低温活性フォスファターゼの低温での効率より機能発現に関わる要因 (2008)
  • Author(s): 田宏樹
  • Organizer: 極限微生物学会年会
  • Place of Presentation: 立教大学、東京
  • Year and Date: 2008-11-04
• [Presentation] 低温活性フォスファターゼの低温での効率よい機能発現に関わる要因 (2008)
  • Author(s): 鶴田宏樹
  • Organizer: 極限微生物学会年会
  • Place of Presentation: 立教大学、東京
  • Year and Date: 2008-11-04
• [Remarks] ホームページ
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