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Development of thermostable protein production system using the radioresistant bacterium

Research Project

Project/Area Number 20780079
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionJapan Atomic Energy Agency

Principal Investigator

SATOH Katsuya  Japan Atomic Energy Agency, 量子ビーム応用研究部門, 研究員 (90370402)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords生物工学 / 遺伝子工学 / 放射線抵抗性細菌 / 高度好熱菌 / 耐熱性タンパク質 / タンパク質生産系 / シャトルベクター / プロモーター配列
Research Abstract

Some proteins originated from thermophile had difficulty with production by the conventional method using Escherichia coli as a host cell. In this study, to develop a novel system for thermostable protein production using the radioresistant bacterium, we searched a useful promoter sequence for gene expression and constructed a novel expression plasmid vector. As a result, the production of thermostable protein without insolubilization could be confirmed using a radioresistant bacterium as a host cell.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (27 results)

All 2010 2009 2008 Other

All Journal Article (10 results) (of which Peer Reviewed: 8 results) Presentation (13 results) Remarks (4 results)

  • [Journal Article] Deinococcus aetherius sp. nov., isolated from the stratosphere2010

    • Author(s)
      Yinjie Yang, Takashi Itoh, Shin-ichi Yokobori, Haruo Shimada, Shiho Itahashi, Katsuya Satoh, Hirofumi Ohba, Issay Narumi, Akihiko Yamagishi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology Vol.60,No.4

      Pages: 776-779

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deinococcus aetherius sp.nov., isolated from the stratosphere2010

    • Author(s)
      Yinjie Yang
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 60

      Pages: 776-779

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deinococcus aerius sp. nov., isolated from the high atmosphere2009

    • Author(s)
      Yinjie Yang, Takashi Itoh, Shin-ichi Yokobori, Shiho Itahashi, Haruo Shimada, Katsuya Satoh, Hirofumi Ohba, Issay Narumi, Akihiko Yamagishi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology Vol.59,No.8

      Pages: 1862-1866

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of versatile shuttle vectors for Deinococcus grandis2009

    • Author(s)
      Katsuya Satoh, Zhenli Tu, Hirofumi Ohba, Issay Narumi
    • Journal Title

      Plasmid Vol.62,No.1

      Pages: 1-9

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Issay Narumi, Identification of PprM: a modulator of the PprI-dependent DNA damage response in Deinococcus radiodurans2009

    • Author(s)
      Hirofumi Ohba, Katsuya Satoh, Haitham Sghaier, Tadashi Yanagisawa
    • Journal Title

      Extremophiles Vol.13,No.3

      Pages: 471-479

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of PprM : a modulator of the PprI-dependent DNA damage response in Deinococcus radiodurans2009

    • Author(s)
      Hirofumi Ohba
    • Journal Title

      Extremophiles 13

      Pages: 471-479

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of versatile shuttle vectors for Deinococcus grandis2009

    • Author(s)
      Katsuya Satoh
    • Journal Title

      Plasmid 62

      Pages: 1-9

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deinococcus aerius sp.nov., isolated from the high atmosphere2009

    • Author(s)
      Yinjie Yang
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 59

      Pages: 1862-1866

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 放射線抵抗性細菌の放射線耐性の謎に迫る2008

    • Author(s)
      大庭寛史、佐藤勝也、鳴海一成
    • Journal Title

      放射線と産業 Vol.118

      Pages: 50-53

    • Related Report
      2009 Final Research Report
  • [Journal Article] 放射線抵抗性細菌の超放射線耐性の謎に迫る2008

    • Author(s)
      大庭寛史
    • Journal Title

      放射線と産業 118

      Pages: 50-53

    • Related Report
      2008 Annual Research Report
  • [Presentation] 放射線抵抗性細菌における新規遺伝子操作系の開発2009

    • Author(s)
      佐藤勝也
    • Organizer
      第10回極限環境微生物学会年会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 放射線抵抗性細菌由来DNA修復促進タンパク質PprAの構造機能解析2009

    • Author(s)
      山田貢
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 放射線抵抗性細菌のプラスミドを用いた新規遺伝子操作系の開発2009

    • Author(s)
      佐藤勝也
    • Organizer
      第4回高崎量子応用研究シンポジウム
    • Place of Presentation
      高崎
    • Year and Date
      2009-10-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] DNA鎖切断の効率的検出法2009

    • Author(s)
      佐藤勝也
    • Organizer
      第8回産学官連携推進会議
    • Place of Presentation
      京都
    • Year and Date
      2009-06-20
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] プロテオーム解析による新規DNA修復関連タンパク質の同定2008

    • Author(s)
      大庭寛史
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2009 Final Research Report
  • [Presentation] プロテオーム解析による新規DNA修復関連タンパク質の同定2008

    • Author(s)
      大庭寛史
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 放射線抵抗性細菌デイノコッカス・ラジオデュランスの放射線応答機構に関与する新規タンパク質の発見2008

    • Author(s)
      大庭寛史
    • Organizer
      第3回高崎量子応用研究シンポジウム
    • Place of Presentation
      高崎
    • Year and Date
      2008-10-10
    • Related Report
      2009 Final Research Report
  • [Presentation] 放射線抵抗性細菌デイノコッカス・ラジオデュランスの放射線応答機構に関与する新規タンパク質の発見2008

    • Author(s)
      大庭寛史
    • Organizer
      第3回高崎量子応用研究シンポジウム
    • Place of Presentation
      高崎市
    • Year and Date
      2008-10-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 放射線抵抗性細菌のDNA修復機構の研究2008

    • Author(s)
      佐藤勝也
    • Organizer
      第39回UV/EB研究会
    • Place of Presentation
      大阪
    • Year and Date
      2008-09-24
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Role of RecF in radiation-induced DNA double-strand breaks repair pathway in Deinococcus radiodurans2008

    • Author(s)
      Katsuya Satoh
    • Organizer
      The 7th International conference on Extremophiles (Extremophiles 2008)
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2008-09-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Functional characterization of a novel modulator for radiation response mechanism in Deinococcus radiodurans2008

    • Author(s)
      Hirofumi Ohba
    • Organizer
      The 7th International conference on Extremophiles (Extremophiles 2008)
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2008-09-09
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Three-dimensional architecture of the Deinococcus radiodurans PprA protein2008

    • Author(s)
      Issay Narumi
    • Organizer
      The 7th International conference on Extremophiles (Extremophiles 2008)
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2008-09-09
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Role of RecF in radiation-induced DNA double-strand breaks repair pathway inDeinococcus radiodurans2008

    • Author(s)
      Katsuya Satoh
    • Organizer
      The 7th International conference on Extremophiles (Extremophiles 2008)
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2008-09-09
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.taka.jaea.go.jp/rab_div/grr/index_j.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.taka.jaea.go.jp/rab_div/grr/index_j.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://yayoi.kansai.jaea.go.jp/biou/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.taka.jaea.go.jp/rab_div/grr/index_j.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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