| Project/Area Number |
20780079
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
SATOH Katsuya Japan Atomic Energy Agency, 量子ビーム応用研究部門, 研究員 (90370402)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 生物工学 / 遺伝子工学 / 放射線抵抗性細菌 / 高度好熱菌 / 耐熱性タンパク質 / タンパク質生産系 / シャトルベクター / プロモーター配列 |
|---|
| Research Abstract |
Some proteins originated from thermophile had difficulty with production by the conventional method using Escherichia coli as a host cell. In this study, to develop a novel system for thermostable protein production using the radioresistant bacterium, we searched a useful promoter sequence for gene expression and constructed a novel expression plasmid vector. As a result, the production of thermostable protein without insolubilization could be confirmed using a radioresistant bacterium as a host cell.
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