| Project/Area Number |
20780083
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
ARAKAWA Kenji Hiroshima University, 大学院・先端物質科学研究科, 助教 (80346527)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 生合成 / 抗生物質 / ポリケチド / 制御遺伝子 / 転写活性化因子 / 糖転移酵素 / アミンオキシダーゼ / 大員環 / ポリケチド合成酵素 / P450 / ガンマブチロラクトン |
|---|
| Research Abstract |
We analyzed the function of the secondary metabolites genes located on the linear plasmid pSLA2-L. In LC biosynthesis, the C-18 amine group in an acyclic compound is oxidized by LkcE to form the iminium intermediate, which then receives the nucleophilic attack from C-2 enolate to form the 17-membered macrocyclic LC. We also determined the order of post-PKS modification in LM biosynthesis. The extensive transcriptional analysis has revealed that the γ-butyrolactone signaling cascade goes from srrX through srrA to srrY, leading to LC and LM production.
|
