Analysis of the biosynthetic pathway and the regulatory mechanism of secondary metabolites in Streptomyces
| Project/Area Number |
20780083
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
ARAKAWA Kenji Hiroshima University, 大学院・先端物質科学研究科, 助教 (80346527)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 生合成 / 抗生物質 / ポリケチド / 制御遺伝子 / 転写活性化因子 / 糖転移酵素 / アミンオキシダーゼ / 大員環 / ポリケチド合成酵素 / P450 / ガンマブチロラクトン |
|---|
| Research Abstract |
We analyzed the function of the secondary metabolites genes located on the linear plasmid pSLA2-L. In LC biosynthesis, the C-18 amine group in an acyclic compound is oxidized by LkcE to form the iminium intermediate, which then receives the nucleophilic attack from C-2 enolate to form the 17-membered macrocyclic LC. We also determined the order of post-PKS modification in LM biosynthesis. The extensive transcriptional analysis has revealed that the γ-butyrolactone signaling cascade goes from srrX through srrA to srrY, leading to LC and LM production.
|
Report
(3 results)
Research Products
(39 results)
-
-
[Journal Article] The structure of ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics2010
Author(s)
T. Auerbach, I. Mermershtain, C. Davidovich, A. Bashan, M. Belousoff, I. Wekselman, E. Zimmerman, L. Xiong, D. Klepacki, K. Arakawa, H. Kinashi, A. Mankin, A. Yonath
-
Journal Title
Proc. Natl. Acad. Sci. USA 107
Pages: 1983-1988
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
