Cloning and application of cannabinoid biosynthetic genes based on EST analysis
| Project/Area Number |
20780084
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
TAURA Futoshi Kyushu University, 大学院・薬学研究院, 助教 (00301341)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 生合成 / EST / カンナビノイド / 遺伝子クローニング |
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| Research Abstract |
In this study, I cloned a gene encoding oli vetol synthase, the polyketide synthase that synthesizes the phenolic moiety of cannabi noids in Cannabis sativa, by means of EST analysis and RT-PCR, and then characterized the bacterially expressed recombinant oli vetol synthase to clarify the structural and functional properties in detail. In addition, I attempted to obtaina gene for the prenyltransferase that synthesize the carbon skeleton of cannabinoids, resultingincloning of two candi date genes for the prenyltransferase. Interestingly, I confirmed that these putativeprenyltransferases showmore that 50% identity with the prenyltransferase involved in secondary net abolismin hop, a close relative of.C. sative.
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Report
(3 results)
Research Products
(15 results)
