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Cloning and application of cannabinoid biosynthetic genes based on EST analysis

Research Project

Project/Area Number 20780084
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionKyushu University

Principal Investigator

TAURA Futoshi  Kyushu University, 大学院・薬学研究院, 助教 (00301341)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords生合成 / EST / カンナビノイド / 遺伝子クローニング
Research Abstract

In this study, I cloned a gene encoding oli vetol synthase, the polyketide synthase that synthesizes the phenolic moiety of cannabi noids in Cannabis sativa, by means of EST analysis and RT-PCR, and then characterized the bacterially expressed recombinant oli vetol synthase to clarify the structural and functional properties in detail. In addition, I attempted to obtaina gene for the prenyltransferase that synthesize the carbon skeleton of cannabinoids, resultingincloning of two candi date genes for the prenyltransferase. Interestingly, I confirmed that these putativeprenyltransferases showmore that 50% identity with the prenyltransferase involved in secondary net abolismin hop, a close relative of.C. sative.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (15 results)

All 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (4 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Characterization of olivetol synt hase, a polyketide synthase put atively involved in cannabinoid biosynthetic pathway2009

    • Author(s)
      F. Taura, S. Tanaka, C. Taguchi, T. Fukamizu, H Tanaka, Y. Shoyama, S. Morimoto
    • Journal Title

      FEBS letters 583

      Pages: 2061-2066

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Studies on tetrahydrocannabi nolic acid synt hase that produces the acidic precursor of tetrahydrocannabinol, the pharnatcologically active cannabi noid in narijuana2009

    • Author(s)
      F. Taura
    • Journal Title

      Drug Discoveries and Therapeutics 3

      Pages: 83-87

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway2009

    • Author(s)
      F.Taura, S.Tanaka, C.Taguchi, T. Fukamizu, H.Tanaka, Y.Shoyama, S.Morimoto
    • Journal Title

      FEBS Letters 583

      Pages: 2061-2066

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on tetrahydrocannabinolic acid synthase that produces the acidic precursor of tetrahydrocannabinol, the pharmacologically active can nabinoid in marijuana2009

    • Author(s)
      F.Taura
    • Journal Title

      Drug Discoveries & Therapeutics 3

      Pages: 83-87

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Grystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa.2008

    • Author(s)
      C. Taguchi, F. Tatra, T. Tanada, Y. Shoyana, Y. Shoyana, H. Tanaka, R. Kiroki, S. Morinoto
    • Journal Title

      Acta Crystallographica Section F: Structural Biology and Crystallization Communications 64

      Pages: 217-220

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of the biosynthetic enzyne for the biotechnological production of tetrahydrocannabinol, the active constituent of narijuana2008

    • Author(s)
      F. Taura
    • Journal Title

      Drug Discoveries and Therapeutics 2

      Pages: 59-59

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray diffraction studies of polyketide synthase from Cannabis sativa2008

    • Author(s)
      Taguchi, C., Taura, F., et.al.
    • Journal Title

      Acta Cryst., F 64

      Pages: 217-220

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of the biosynthetic enzyme for the biotechnol ogical production of tetrahydrocannabinol, the active constituent of mari juana2008

    • Author(s)
      Taura, F.
    • Journal Title

      Drug Discoveries and Therapeutics 2, Suppl

      Pages: 39-39

    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway2009

    • Author(s)
      F. Taura
    • Organizer
      Asian Synposium for Pharnaceutical Sciences in JSPS Asian Core Programin Fukuoka
    • Place of Presentation
      福岡市
    • Year and Date
      2009-11-06
    • Related Report
      2009 Final Research Report
  • [Presentation] Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway2009

    • Author(s)
      F.Taura
    • Organizer
      Asian Symposium for Pharmaceutical Sciences in JSPS Asian Core Program in Fukuoka
    • Place of Presentation
      福岡市
    • Year and Date
      2009-11-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大麻成分の生合成酵素に関する研究2009

    • Author(s)
      田浦太志
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京者区市
    • Year and Date
      2009-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] 大麻成分の生合成酵素に関する研究2009

    • Author(s)
      田浦太志
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Book] Cannabinoid in Nature and Medicine (DM Iambert (Ed. ))2009

    • Author(s)
      F. Taura, S. Sirikantaramas, Y. Shoyama, Y. Shoyama, S. Morimoto
    • Publisher
      Springer Verlag
    • Related Report
      2009 Final Research Report
  • [Book] Phytocannabinoids in Cannabis sativa : Recent studies on biosynthetic enzymes in : D.M. Lambert (Ed.), Cannabinoid in Nature and Medicine2009

    • Author(s)
      F.Taura, S.Sirikantaramas, Y.Shoyama, Y.Shoyama, S.Morimoto
    • Total Pages
      416
    • Publisher
      Springer Verlag
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://seigyo.phar.kyushu-u.ac.jp/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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