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The in vivo roles of PKD1L3 and PKD2L1 in sour taste detection

Research Project

Project/Area Number 20780089
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionThe University of Tokyo

Principal Investigator

ISHIMARU Yoshiro  The University of Tokyo, 大学院・農学生命科学研究科, 特任助教 (10451840)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords味覚受容体 / 酸味 / TRPチャネル / シグナル伝達 / 遺伝子破壊マウス / 味神経応答解析 / カルシウムイメージング解析
Research Abstract

To elucidate the physiological roles of a candidate sour taste receptor, PKD1L3/PKD2L1, we generated gene knockout mice for PKD1L3 and/or PKD2L1. To investigate phenotype of these mice in taste sensation including sour taste, behavioral and electrophysiological analyses were performed. In the behavioral analyses such as two-bottle preference test and brief-access licking test, PKD1L3, PKD2L1, and double KO mice showed normal preference or avoidance behavior similar to the wild-type littermates for all the five basic taste qualities. In contrast, both whole-nerve and single-fiber chorda tympani nerve responses of PKD2L1 KO and double KO mice to sour solutions such as citric acid, HCl, and acetic acid significantly reduced compared with wild-type mice, while PKD1L3 KO mice showed normal responses. In glossopharyngeal nerve recordings, PKD1L3, PKD2L1, and double KO mice showed normal responses compared with wild-type mice. In addition, PKD1L3, PKD2L1, and double KO mice showed responses s … More imilar to wild-type mice upon stimulation with salt,sweet, bitter, and umami (savory) taste compounds. Collectively, these results demonstrate that PKD2L1 functions as a sour taste receptor in vivo and suggest that there are multiple mechanisms underlying sour taste detection such as somatosensation other than via taste buds.
We identified the N-terminal extracellular region of human PKD1L3 and the full-length of human PKD2L1. HEK 293T cells expressing human PKD2L1 and chimeric PKD1L3, which consists of the N-terminal extracellular region of human PKD1L3 and the following region of mouse PKD1L3, showed off-response upon stimulation with sour solution such as citric acid. These cells still showed robust response to sour stimuli in the presence of a taste-modifying protein, neoculin, extracted from Curculigo latifolia. These results raise the possibility that neoculin inhibits the region other than the N-terminal extracellular region of human PKD1L3. Alternatively, inhibition event occurs at the level of taste cells or taste neurons, not at the taste receptors. Less

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (28 results)

All 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (13 results) Remarks (3 results)

  • [Journal Article] Signal peptide peptidases are expressed in the shoot apex of rice, localized to the endoplasmic reticulum2009

    • Author(s)
      Tamura T, Kuroda M, Oikawa T, Kyozuka J, Terauchi K, Ishimaru Y, Abe K, Asakura T
    • Journal Title

      Plant Cell Rep. 28巻

      Pages: 1615-1621

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acetic acid activates PKD1L3-PKD2L1 channel - a candidate sour taste receptor2009

    • Author(s)
      Ishii S, Misaka T, Kishi M, Kaga T, Ishimaru Y, Abe K.
    • Journal Title

      Biochem. Biophys. Res. Commun. 385巻

      Pages: 346-350

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of taste transduction in vertebrates2009

    • Author(s)
      Ishimaru Y.
    • Journal Title

      Odontology 97巻

      Pages: 1-7

    • NAID

      10027301053

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transient receptor potential (TRP) channels and taste sensation2009

    • Author(s)
      Ishimaru Y, Matsunami H.
    • Journal Title

      J. Dent. Res. 88巻

      Pages: 212-218

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] pH-Dependent Inhibition of the Human Bitter Taste Receptor hTAS2R16 by a Variety of Acidic Substances2009

    • Author(s)
      Sakurai T, MisakaT, Nagai T, Ishimaru Y, Matsuo S, Asakura T, Abe K.
    • Journal Title

      J. Agric. Food Chem. 57巻

      Pages: 2508-2514

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acetic acid activates PKD1L3-PKD2L1 channel - a candidate sour taste receptor2009

    • Author(s)
      Ishii, S., et al
    • Journal Title

      Biochem.Biophys.Res.Commun. 385

      Pages: 346-350

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Signal peptide peptidases are expressed in the shoot apex of rice, localized to the endoplasmic reticulum2009

    • Author(s)
      Tamura, T., et al
    • Journal Title

      Plant Cell Rep. 28

      Pages: 1615-1621

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of taste transduction in vertebrates2009

    • Author(s)
      Ishimaru, Y.
    • Journal Title

      Odontology 97

      Pages: 1-7

    • NAID

      10027301053

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient receptor potential (TRP) channels and taste sensation.2009

    • Author(s)
      Ishimaru, Y. & Matsunami, H.
    • Journal Title

      J. Dent. Res. 88

      Pages: 212-218

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] pH-Dependent Inhibition of the Human Bitter Taste Receptor hTAS2R16 by a Variety of Acidic Substances.2009

    • Author(s)
      Sakurai T. et.al
    • Journal Title

      J. Agric. Food. Cheni. 57

      Pages: 2508-2514

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L12008

    • Author(s)
      Inada H, Kawabata F, Ishimaru Y, Fushiki T, Matsunami H, Tominaga M.
    • Journal Title

      EMBO Reports 9巻

      Pages: 690-697

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1.2008

    • Author(s)
      Inada, H.,et.al
    • Journal Title

      EMBO Reports 9

      Pages: 690-697

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 酢酸刺激に対する培養細胞の応答の蛍光イメージングによる解析2010

    • Author(s)
      石井翔、三坂巧、岸幹也、山上圭吾、石丸喜朗、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ヒト苦味受容体hTAS2R16のリガンド受容部位の解析2010

    • Author(s)
      櫻井敬展、石黒正路、三坂巧、石丸喜朗、朝倉富子、松尾伸二、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 酸味受容体候補PKD1L3/PKD2L1遺伝子破壊マウスの作出と行動学的表現型解析2010

    • Author(s)
      石丸喜朗、秋場雅人、成川真隆、朝倉富子、松波宏明、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 哺乳類酸味受容体PKD1L3/PKD2L1の構造・機能相関解析2010

    • Author(s)
      片野佑香、石丸喜朗、朝倉富子、松波宏明、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 酸味情報伝達神経回路の可視化2010

    • Author(s)
      山本くるみ、石丸喜朗、應本真、朝倉富子、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] グリシニン欠失大豆の特性と登熟期における遺伝子発現の網羅的解析2010

    • Author(s)
      田村倫子、成川知世、石丸喜朗、舟木淳子、阿部啓子、朝倉富子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 施肥量の異なる区で栽培されたイネ種子における遺伝子発現の網羅的解析2010

    • Author(s)
      成川知世、黒田昌治、石丸喜朗、阿部啓子、朝倉富子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 哺乳類酸味受容体PKD1L3/PKD2L1のサブユニット間相互作用領域の解析2009

    • Author(s)
      片野佑香、石丸喜朗、朝倉富子、松波宏明、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      博多
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 酢酸に対する酸味受容体候補分子PKD1L3/PKD2L1の応答測定条件の最適化2009

    • Author(s)
      石井翔、三坂巧、岸幹也、加賀孝之、石丸喜朗、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      博多
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] ヒト苦味受容体のリガンド認識はpH低下とともに抑制される2009

    • Author(s)
      櫻井敬展、三坂巧、石丸喜朗、朝倉富子、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      博多
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 大豆種子成熟過程における発現遺伝子の網羅的解析2009

    • Author(s)
      成川知世、田村倫子、寺内かえで、石丸喜朗、阿部啓子、朝倉富子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      博多
    • Year and Date
      2009-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] イネの膜結合型酵素シグナルペプチドペプチダーゼの発現解析2009

    • Author(s)
      田村倫子、黒田昌治、及川鉄男、中井雄治、石丸喜朗、三坂巧、経塚淳子、阿部啓子、朝倉富子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      博多
    • Year and Date
      2009-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] PKD1L3/PKD2L1チャネル複合体による酸味受容の分子機構2008

    • Author(s)
      富永真琴、稲田仁、石丸喜朗、松波宏明
    • Organizer
      中部日本生理学会
    • Place of Presentation
      愛知
    • Year and Date
      2008-10-17
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/tastescience/index.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/tastescience/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/tastescience/index.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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