Searching for susceptible genes acting metabolic disorders induced by a high-fat diet

• Project/Area Number: 20780093
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: Nagoya University
• Principal Investigator: KOBAYASHI Misato Nagoya University, 大学院・生命農学研究科, 助教 (20456586)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 高脂肪食 / 脂質代謝 / 脂肪肝 / コンソミック / マウス / 血中インスリン / QTL / 遺伝解析 / 糖代謝 / 耐糖能

Research Abstract

To identify susceptible genes for diet-induced obesity, dyslipidemia, and impaired glucose metabolism, we used mouse chromosome substitution strains (consomic) which is a powerful animal model for genetic analyses. Consomic mouse showed that gene(s) on chromosome 12 control susceptibility for high-fat diet induced not only fatty liver but also insulin resistance. In addition, we could select one candidate gene for these traits using gene expression analysis in liver.

Research Products

• [Journal Article] Confirmation of diabetes-related quantitative trait loci derived from SM/J and A/J mice by using congenic strains fed a high-carbohydrate or high-fat diet. (2009)
  • Author(s): Kobayashi M, Hada N, Hoshino H, Ozawa T, Umeshita K, Nishimura H, Murai A, Ohno T, Horio F.
  • Journal Title: J NUtr Sci Vitaminol (Tokyo) vol.55 Pages: 257-63
  • NAID: 10025971398
  • Peer Reviewed
• [Journal Article] Confirmation of diabetes-related quantitative trait loci derived from SM/J and A/J mice by using congenic strains fed a high-carbohydrate or high-fat diet. (2009)
  • Author(s): Kobayashi M, Hada N, et al.
  • Journal Title: J Nutr Sci Vitaminol (Tokyo) 55(3) Pages: 257-263
  • NAID: 10025971398
  • Peer Reviewed
• [Journal Article] Quantitative trait loci for impaired glucose tolerance in nondiabetic SM/J and A/J mice. (2008)
  • Author(s): Hada N, Kobayashi H, Fujiyoshi M, Ishikawa A, Kuga M, Nishimura M, Ebihara S, Ohno T, Horio F.
  • Journal Title: Physiol Ganomics vol.35 Pages: 65-74
  • Peer Reviewed
• [Journal Article] Quantitative trait loci for impaired glucose tolerance in nondiabetic SM/J and A/J mice (2008)
  • Author(s): 羽田奈津子
  • Journal Title: Physiol Genomics 35(1) Pages: 65-74
  • Peer Reviewed
• [Presentation] マウス第12番染色体に存在する脂肪肝感受性遺伝子の遺伝子発現解析による探索 (2010)
  • Author(s): 小林美里, 他
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キヤンパス
  • Year and Date: 2010-03-28
• [Presentation] マウス第12番染色体に存在する脂肪肝感受性遺伝子の遺伝子発現解析による探索 (2010)
  • Author(s): 小林美里, 田口千恵, ら
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-28
• [Presentation] マウス第12番染色体に存在する脂肪肝感受性遺伝子座の解析 (2010)
  • Author(s): 小林美里, 他
  • Organizer: 第24回日本糖尿病・肥満動物学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2010-01-22
• [Presentation] 生活習慣病に関わる遺伝因子と食事因子 (2009)
  • Author(s): 小林美里, 他
  • Organizer: 第14回日本フードファクター学会
  • Place of Presentation: 神戸大学六甲台キャンパス
  • Year and Date: 2009-11-17
• [Presentation] コンソミックマウスを用いた第12番染色体上の脂肪肝原因遺伝子の探索 (2009)
  • Author(s): 田口千恵、小林美里, 他
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28