Analysis of lipid metabolites produced by gut flora and studies on the mechanism of anti-inflammatory activity exerted by the compounds

• Project/Area Number: 20780096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: Kobe University
• Principal Investigator: NISHITANI Yosuke Kobe University, 自然科学系先端融合研究環重点研究部, 助教 (80457093)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 腸内細菌 / 脂質代謝産物 / 腸管免疫 / 脂質代謝

Research Abstract

It is known that various lipids are metabolized in vivo, and act as physiologically active substances. However, the participation of gut flora to lipid metabolism and the effect of lipid metabolites on gut immune system have yet to be fully elucidated. In this study, we tried to analyze lipid metabolites produced by intestinal microflora by using LC-MS/MS, though it had yet to be succeeded because of the effect of impurities. Meanwhile, we revealed that known lipid metabolites, including lipoxin A4 and resolvin E1, exert anti-inflammatory activity in intestinal tract.

Research Products

• [Journal Article] Lipoxin A(4) reduces lipopolysaccharide-induced inflammation in macrophages and intestinal epithelial cells through inhibition of nuclear factor-kappaB activation. (2010)
  • Author(s): Kure I、Nishiumi S、Nishitani Y, 他8名
  • Journal Title: J Pharmacol Exp Ther. 332 Pages: 541-548
  • Peer Reviewed
• [Journal Article] Resolvin E1, an endogenous lipid mediator derived from eicosapentaenoic acid, prevents dextran sulfate sodium-induced colitis. (2010)
  • Author(s): Ishida T、Yoshida M、Arita M、Nishitani Y, 他10名
  • Journal Title: Inflamm Bowel Dis. 16 Pages: 87-95
  • Peer Reviewed
• [Journal Article] Lipoxin A4 reduces lipopolysaccharide-induced inflammation in macrophages and intestial epithelial cells through inhibition of NF-κB activation. (2010)
  • Author(s): Kure, I., Nishiumi, S., Nishitani, Y., Tanoue, T., Ishida, T., Mizuno, M., Fujita, T., Kutsumi, H., Arita, M., Azuma, T., Yoshida, M.
  • Journal Title: J.Pharmacol. Exp.Ther. 332 Pages: 541-548
  • Peer Reviewed
• [Journal Article] Resolvin E1, an endogenous lipid mediator derived from eicosapentaenoic acid, prevents dextran sulfate sodium-induced colitis. (2010)
  • Author(s): Ishida, T., Yoshida, M., Arita, M., Nishitani, Y., Nishiumi, S., Masuda, A., Mizuno, S., Takagawa, T., Morita, Y., Kutsumi, H., Inokuchi, H., Serhan, C.N., Blumberg, R.S., Azuma, T.
  • Journal Title: Inflamm.Bowel Dis. 16 Pages: 87-95
  • Peer Reviewed
• [Presentation] EPA由来生理活性物質の抗炎症作用 (2008)
  • Author(s): 西谷洋輔
  • Organizer: 日本農芸化学会関西支部第454回講演会
  • Place of Presentation: 京都府立大学
  • Year and Date: 2008-05-31