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Elucidation of biofunctional expression mechanism of food polyphenols by oxidative modification of protein

Research Project

Project/Area Number 20780101
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionOsaka Prefecture University

Principal Investigator

AKAGAWA Mitsugu  Osaka Prefecture University, 生命環境科学研究科, 助教 (70405356)

Co-Investigator(Renkei-kenkyūsha) ISHII Takeshi  静岡県立大学, 大学院・生活健康科学研究科, 助教 (50448700)
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsポリフェノール / カテキン / 緑茶 / ガン / 蛋白質 / p68 / エピガロカテキンがレート / エピガロカテキンガレート / ミリセチン
Research Abstract

In the present study, we searched for polyphenols that covalently bind to cellular proteins. A major green tea catechin, epigallocatechin-3-gallate (EGCG), was identified to bind to cellular proteins with high affinity. The regulation mechanism of protein function by the binding of EGCG was examined. We also identified cellular protein targets of EGCG in several functional categories including energy metabolism, glycolysis, chaperone, and RNA processing. The binding of EGCG to the target protein was suggested to be closely involved in anti-cancer effect of EGCG.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (29 results)

All 2010 2009 2008

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (18 results)

  • [Journal Article] Pro-oxidant action of pyrroloquinoline quinone : characterization of protein oxidative modifications.2010

    • Author(s)
      Ishii T, Akagawa M, Naito Y, Handa O, Takagi T, Mori T, Kumazawa S, Yoshikawa T, Nakayama T.
    • Journal Title

      Biosci. Biotechnol. Biochem. 74

      Pages: 663-666

    • NAID

      10027553208

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent detection of a-aminoadipic and g-glutamic semialdehydes in oxidized proteins2009

    • Author(s)
      Akagawa, M., Suyama, K., Uchida, K.
    • Journal Title

      Free Radic. Biol. Med. 46(6)

      Pages: 701-706

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Catechol-type polyphenol is a potential modifier of protein sulfhydryls: Development and application of a new probe for understanding the dietary polyphenol actions2009

    • Author(s)
      Ishii, T., Ishikawa, M., Miyoshi, N., Yasunaga, M., Akagawa, M., Uchida, K., Nakamura, Y.
    • Journal Title

      Chem. Res. Toxicol. 22(10)

      Pages: 1689-1698

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Catechol type polyphenol is a potential modifier of protein sulfhydryls : development and application of a new probe for understanding the dietary polyphenol actions.2009

    • Author(s)
      Ishii T, Ishikawa M, Miyoshi N, Yasunaga M, Akagawa M, Uchida K, Nakamura Y.
    • Journal Title

      Chem.Res.Toxicol. 22

      Pages: 1689-1698

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stereochemical configuration of 4-hydroxy-2-nonenal-cysteine adducts and their stereoselective formation in a redox-regulated pmtein.2009

    • Author(s)
      Wakita C, Maeshima T, Yamazaki A, Shibata T, Ito S, Akagawa M, Ojika M, Yodoi J, Uchida K.
    • Journal Title

      J.BioI.Chem. 284

      Pages: 28810-2822

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent detection of a-aminoadipic and y-glutamic semialdehydes in oxidized protein2009

    • Author(s)
      Akagawa, M., Suyama, K., and Uchida, K
    • Journal Title

      Free Radic. Biol. Med 46

      Pages: 701-705

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Covalent modification of proteins by green tea polyphenol (-)-epigallocatechin-3-gallate through autoxidation2008

    • Author(s)
      Ishii, T., Mori, T., Tanaka, T., Mizuno, D., Yamaji, R., Kumazawa, S., Nakayama, T., Akagawa, M.
    • Journal Title

      Free Radic. Biol. Med. 45(10)

      Pages: 1384-1394

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypoallergenic Characteristics of Wheat Flour Produced by Stepwise Polishing2008

    • Author(s)
      Handoyo, T., Akagawa, M., Morita, N., Maeda, T., Mitsunaga, T
    • Journal Title

      Intern. J. Food Proper 11

      Pages: 243-252

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein oxidative modification with pyrroloquinoline quinone2008

    • Author(s)
      Ishii, T., Mori, T., Kumazawa, S., Nakayama, T., and Akagawa, M
    • Journal Title

      J. Clin. Biochem. Nutr 43

      Pages: 571-574

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mass spectrometry-based study of protein redox modification by alpha-lipoic acid2008

    • Author(s)
      Mori, T., Wakabayashi, M., Ishii, T., Akagawa, M., and Nakayama, T
    • Journal Title

      J. Clin. Biochem. Nutr 43

      Pages: 456-459

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Covalent modification of proteins by green tea polyphenol(-)-epigallocate chin-3-gallate through autoxidation2008

    • Author(s)
      Ishii, T., Mori, T., Tanaka, T., Mizuno, D., Yamaji, R., Kumazawa, S., Nakayama, T., and Akagawa, M
    • Journal Title

      Free Radic_ Biol. Med 45

      Pages: 1384-1394

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] ピロロキノリンキノン(PQQ)による細胞増殖促進機構の解析2010

    • Author(s)
      木村一城、高田真由美、石井剛志、内藤健太郎、赤川貢
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] カテキン類とプロテアソームとの反応性2009

    • Author(s)
      森大気、石井剛志、赤川貢、中山勉
    • Organizer
      第14回日本フードファクター学会
    • Place of Presentation
      神戸
    • Year and Date
      2009-11-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] カテキン類とグルタチオン-S-転移酵素P1-1との共有結合反応2009

    • Author(s)
      森大気、石井剛志、赤川貢、中村宜督、中山勉
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] α-リボ酸のリンクルケア効果の解明へのアプローチ2009

    • Author(s)
      石倉聖子、佐伯裕史、赤川貢、内藤健太郎
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Epigallocatechin-3-gallateの上皮成長因子受容体への結合による癌細胞の増殖抑制機構2009

    • Author(s)
      田中智子、前田智裕、木村一城、久保嘉輝、石井剛志、赤川貢
    • Organizer
      第6回日本カテキン学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 飲料ポリフェノールのアミンオキシダーゼ様活性に関する研究2009

    • Author(s)
      赤川貢、重光智子、須山享三
    • Organizer
      第6回日本カテキン学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] カテキン類のB環、ガロイル基及びC環2, 3位の立体配置が蛋白質システイン残基との共有結合反応に及ぼす影響2009

    • Author(s)
      森大気、石井剛志、赤川貢、中村宜督、中山勉
    • Organizer
      第6回日本カテキン学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] カテキン類と相互作用する血清タンパク質の網羅的探索2009

    • Author(s)
      勝間田知治、石井剛志、森大気、赤川貢、中山勉
    • Organizer
      第6回日本カテキン学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 日本農芸化学会2009年度大会2009

    • Author(s)
      石井剛志、若林美由紀、伊藤創平、赤川貢、中山勉
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] α-トリポ酸による蛋白質のレドックス制御反応2009

    • Author(s)
      瓦谷みのり、石井剛志、若林美由紀、森大気、中山勉、赤川貢
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒト血清アルブミンによるエピガロカテキンガレートの酸化抑制機構2009

    • Author(s)
      市川達也、石井剛志、伊藤創平、赤川貢、中山勉
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 還元的アミノ化反応を用いたタンパク質カルボニルのプロテオミクス解析2009

    • Author(s)
      脇田知佳、石野孔祐、山横敦史、柴田貴広、赤川貢、内田浩二
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 培養細胞系におけるepigallocatechin-3-gallate結合タンパク質の解析2008

    • Author(s)
      田中智子、石井剛志、水野大輔、森大気、熊澤茂則、中山勉、赤川貢
    • Organizer
      第13回日本フードファクター学会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-17
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] (-)epigallocatechin gallateとglutathion-S-transferase P1-1との共有結合反応2008

    • Author(s)
      森大気、石井剛志、田中智子、赤川貢、中山勉
    • Organizer
      第13回日本フードファクター学会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] 蛋白質システイン残基との共有結合反応に関するカテキン類の酸化特性2008

    • Author(s)
      若林美由紀、石井剛志、森大気、赤川貢、中山勉
    • Organizer
      日本農芸化学会中部支部第153回例会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-11-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] Formation of Phosphatidylglycolaldehyde, A Novel Aldehydic Phospholipid, by Oxidative Deamination of Phosphatidylethanolamine2008

    • Author(s)
      Mitsugu Akagawa, Takaaki Kondo, Masaharu Narike, Koji Uchida, and Kyozo Suyama
    • Organizer
      The international Symposium on Lipid Peroxidation 2008
    • Place of Presentation
      軽井沢
    • Year and Date
      2008-10-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] リジン残基の酸化的脱アミノ化によるタンパク質の架橋化反応2008

    • Author(s)
      赤川貢
    • Organizer
      第27回静岡生命科学若手セミナー
    • Place of Presentation
      静岡
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] カテキン類の酸化安定性と蛋白質システイン残基との反応性2008

    • Author(s)
      森大気、石井剛志、赤川貢、中山勉
    • Organizer
      日本酸化ストレス学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2008-06-20
    • Related Report
      2009 Final Research Report 2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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