Development of an assessment system of probiotics based on host-microbial crosstalk information

• Project/Area Number: 20780105
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FUKUDA Shinji The Institute of Physical and Chemical Research, 免疫系構築研究チーム, 基礎科学特別研究員 (80435677)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: プロバイオティクス / 腸内細菌 / メタボローム / トランスクリプトーム / ゲノム / オミクス / 相互作用 / ビフィズス菌 / 代謝物プロファイリング / 複合オミクス / 遺伝子発現プロファイリング / 複合オミックス / 遺伝子発現プロフティリング

Research Abstract

To elucidate the prevention mechanisms by probiotic bifidobacteria against E. coli O157:H7 lethal infection, we took advantage of multi-omics-based approach. Detailed research revealed that the fecal concentration of short chain fatty acid (SCFA) in probiotic bifidobacteria-associated mice was significantly higher than that in non-probiotic bifidobacteria-associated mice. SCFA could inhibit colonic epithelial cells from cell death provoked by O157 infection, suggesting that the abilities of sugar metabolism and SCFA production in probiotic bifidobacteria play a critical role in the prevention of O157 lethal infection.

Research Products

• [Journal Article] Identification of TOSO/FAIM3 as an Fc receptor for IgM (2010)
  • Author(s): Shima, H. *, Takatsu, H. *, Fukuda, S., Ohmae, M., Hase, K., Kubagawa, H., Wang, J.Y., Ohno, H.
  • Journal Title: Int. Immunol 22 Pages: 149-156
  • NAID: 10027391592
  • Peer Reviewed
• [Journal Article] Evaluation and characterization of bacterial metabolic dynamics with a novel profiling technique, real-time metabolotyping (2009)
  • Author(s): Fukuda, S., Nakanishi, Y., Chikayama, E., Ohno, H., Hino, T., Kikuchi, J.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Uptake via Glycoprotein 2 of FimH+ bacteria by M cells initiates mucosal immune response (2009)
  • Author(s): Hase, K., Kawano, K., Nochi, T, Pontes, G.S., Fukuda, S., Ebisawa, M., Kadokura, K., Tobe, T., Fujimura, Y., Kawano, S., Nakato, G., Kimura, S., Murakami, T., Iimura, M., Hamura, K., Fukuoka, S.I., Lowe, A.W., Waguri, S., Itoh, K, Kiyono, H., Ohno, H.
  • Journal Title: Nature 462 Pages: 226-230
  • Peer Reviewed
• [Journal Article] New approach for M-cell-specific molecules screening by comprehensive transcriptome analysis (2009)
  • Author(s): Nakato, G., Fukuda, S., Hase, K., Goitsuka, R., Cooper, DM, Ohno, H.
  • Journal Title: DNA Res. 16 Pages: 227-235
  • Peer Reviewed
• [Journal Article] 腸管出血性大腸菌O157感染症に対するプロバイオティクスの防御効果 (2009)
  • Author(s): 福田真嗣、大野博司
  • Journal Title: 腸内細菌学会誌 23 Pages: 271-278
  • NAID: 10027119289
• [Journal Article] Evaluation and characterization of bacterial metabolic dynamics with a novel profiling technique, real-time metabolotyping (2009)
  • Author(s): 福田真嗣
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] 腸管出血性大腸菌O157感染症に対するプロバイオティクスの防御効果 (2009)
  • Author(s): 福田真嗣
  • Journal Title: 腸内細菌学会誌 23 Pages: 271-278
  • NAID: 10027119289
• [Journal Article] Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production (2008)
  • Author(s): Morita, H., Toh, H., Fukuda, S., Horikawa, H., Oshima, K., Suzuki, T., Murakami, M., Hisamatsu, S., Kato, Y., Takizawa, T., Fukuoka, H., Yoshimura, T., Itoh, K., O'Sullivan, D.J., McKay, L.L., Ohno, H., Kikuchi J., Masaoka, T., Hattori, M.
  • Journal Title: DNA Res. 15 Pages: 151-161
  • Peer Reviewed
• [Journal Article] Development of a monitoring approach for metabolic dynamics in microbial ecosystems by stable isotope labeling technologies
  • Author(s): Date, Y., Nakanishi, Y., Fukuda, S., Kato, T., Tsuneda, S., Ohno, H., Kikuchi, J.
  • Journal Title: J. Biosci. Bioeng. (in press)
  • Peer Reviewed
• [Journal Article] Evaluation and characterization of bacterial metabolic dynamics with a novel pro filing technique, real-time metabolotyping
  • Author(s): Fukuda, S.
  • Journal Title: PLoS ONE (in press)
  • Peer Reviewed
• [Presentation] Gut environment assessment system based on multiple omics approach (2010)
  • Author(s): Fukuda, S., Date, Y., Kato, T., Nakanishi, Y., Chikayama, E., Tsuboi, Y., Tsuneda, S., Moriya, S., Kikuchi, J., Ohno, H.
  • Organizer: National Institute of Health Human Microbiome Project Research Network Meeting
  • Place of Presentation: Houston
• [Presentation] 腸管出血性大腸菌O157:H7感染マウスモデルに対するプロバイオティクスの防御効果の解析 (2010)
  • Author(s): 福田真嗣、大野博司
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] Multi-omic analysis identifies microbial metabolites to protect host from enterohemorrhagic Escherichia coli O157:H7 lethal infection (2009)
  • Author(s): Fukuda, S.
  • Organizer: Keystone Symposia: Innate, Adaptive and Regulatory Immune Response to Intestinal Microbiota
  • Place of Presentation: Taos
  • Year and Date: 2009-01-14
• [Presentation] Multi-omics analysis identifies microbial metabolites to protect host from enterohemorrhagic Escherichia coli O157:H7 lethal infection (2009)
  • Author(s): Fukuda, S., Nakanishi, Y., Toh, H., Yoshimura, K., Oshima, K., Morita, H., Tobe, T., Itoh, K., Kikuchi, J., Hattori, M., Ohno, H.
  • Organizer: Keystone Symposia: Innate, Adaptive and Regulatory Immune Response to Intestinal Microbiota
  • Place of Presentation: Taos
• [Presentation] 複合オミックス解析による腸内環境評価系の構築 (2009)
  • Author(s): 福田真嗣・伊達康博・加藤完・中西裕美子・近山英輔・坪井裕理・常田聡・守屋繁春・菊地淳・大野博司
  • Organizer: 第13回腸内細菌学会
  • Place of Presentation: 東京
• [Presentation] Multiple Omics Identified Microbial Metabolites to Protect Host from Enterohemorrhagic Escherichia coli O157:H7 Lethal Infection (2009)
  • Author(s): Fukuda, S., Nakanishi, Y., Toh, H., Yoshimura, K., Oshima, K., Tobe, T., Morita, H., Itoh, K., Kikuchi, J., Hattori, M., Ohno, H.
  • Organizer: The 8th International Workshop on Advanced Genomics
  • Place of Presentation: Tokyo
• [Presentation] Multiple Omics Identified Microbial Metabolites to Protect Host from Enterohemorrhagic Escherichia coli O157:H7 Lethal Infection (2009)
  • Author(s): Fukuda, S., Nakanishi, Y., Toh, H., Yoshimura, K., Oshima, K., Tobe, T., Morita, H., Itoh, K., Kikuchi, J., Hattori, M., Ohno, H.
  • Organizer: 14th International Congress of Mucosal Immunology
  • Place of Presentation: Boston
• [Presentation] Multiple Omics Identified Microbial Metabolites to Protect Host from Enterohemorrhagic Escherichia coli O157:H7 Lethal Infection (2009)
  • Author(s): Fukuda, S., Hase, K., Itoh, K., Morita, H., Ohno, H.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
• [Presentation] 複合オミックス解析による腸内環境評価系の構築 (2009)
  • Author(s): 福田真嗣・伊達康博・加藤完・中西裕美子・近山英輔・坪井裕理・常田聡・守屋繁春・菊地淳・大野博司
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 腸管出血性大腸菌O157:H7投与マウスモデルを用いた宿主-腸内フローラ間相互作用の解析 (2009)
  • Author(s): 福田真嗣、大野博司
  • Organizer: 第12回日本臨床腸内微生物学会総会
  • Place of Presentation: 岐阜
• [Presentation] 複合オミックス解析による腸内環境評価系の構築 (2009)
  • Author(s): 福田真嗣、大野博司
  • Organizer: 第19回日本数理生物学会年会
  • Place of Presentation: 東京
• [Presentation] Multi-omic analysis identifies microbial metabolites to protect host from enterohemorrhagic Escherichia coli O157:H7 lethal infection (2008)
  • Author(s): Fukuda, S.
  • Organizer: A Global COE International Symposium 「Systems Biology for the Young Scientists」
  • Place of Presentation: Nagoya
  • Year and Date: 2008-12-02
• [Presentation] 腸管出血性大腸菌0157:H7投与マウスモデルを用いた宿主-腸内フローラ間相互作用の解析 (2008)
  • Author(s): 福田真嗣
  • Organizer: 第12回腸内細菌学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-12
• [Presentation] 腸管出血性大腸菌O157:H7投与マウスモデルを用いた宿主-腸内フローラ間相互作用の解析 (2008)
  • Author(s): 福田真嗣・中西裕美子・吉村和敏・伊藤喜久治・菊地淳・大野博司
  • Organizer: 第12回腸内細菌学会
  • Place of Presentation: 東京
• [Presentation] 網羅的解析手法による宿主-腸内フローラ間相互作用の解明 (2008)
  • Author(s): 福田真嗣、大野博司
  • Organizer: 第60回日本生物工学会
  • Place of Presentation: 仙台
• [Presentation] Multi-omic analysis identifies microbial metabolites to protect host from enterohemorrhagic Escherichia coli O157:H7 lethal infection (2008)
  • Author(s): Fukuda, S., Ohno, H.
  • Organizer: ○A Global COE International Symposium「Systems Biology for the Young Scientists」Nagoya
• [Presentation] リンパ濾胞上皮層における宿主-微生物間相互作用の解析 (2008)
  • Author(s): 福田真嗣、大野博司
  • Organizer: 第14回Hindgut Club Japanシンポジウム
  • Place of Presentation: 東京
• [Book] 難培養微生物研究の最新技術II～ゲノム解析を中心とした最前線と将来展望～監修工藤俊章/大熊盛也 (2010)
  • Author(s): 福田真嗣(共著)
  • Publisher: シーエムシー出版
• [Remarks] 福田真嗣、大野博司"腸内フローラと腸管免疫"ネスレ栄養科学会議栄養科学トピックス
  • URL: http://www.nestle.co.jp/science/topics/topics0912_2.asp
• [Patent(Industrial Property Rights)] 腸管バリア機能改善剤 (2009)
  • Inventor(s): 福田真嗣、藤英博、服部正平、大野博司
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2009-204157
  • Filing Date: 2009-09-03
• [Patent(Industrial Property Rights)] IgA腎症の予測方法およびIgA腎症患者の選択方法 (2009)
  • Inventor(s): 長澤康行、飯尾健一郎、猪阪善隆、福田真嗣、大野博司
  • Industrial Property Rights Holder: 国立大学法人大阪大学・独立行政法人理化学研究所
  • Industrial Property Number: 2009-029227
  • Filing Date: 2009-02-12
• [Patent(Industrial Property Rights)] 原核生物、特に真正細菌のcDNAの簡易な調製方法 (2008)
  • Inventor(s): 守屋繁春、福田真嗣、加藤完、大野博司、菊地淳
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2008-052503
  • Filing Date: 2008-03-03