Development of stress diagnosis technology for photosynthesis based on genomic biology

• Project/Area Number: 20780108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Forest science
• Research Institution: Hokkaido University
• Principal Investigator: SAITO Hideyuki Hokkaido University, 大学院・農学研究院, 助教 (70312395)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ストレス診断 / ブナ / ゲノム生態学 / 光合成 / 遺伝子発現

Research Abstract

We firstly generated a normalized and full-length enriched cDNA library of leaves of beech (Fagus crenata Blume). The 5'end one pass sequence for 5,376 clones was analyzed, and 3,629 non-redundant clones were annotated. Second, we evaluated the stability of candidate reference gene mRNA transcript level to seasonal change and various abiotic stresses ; remove of leaf, dry, heat, chilling, hydrogen peroxide, resulting that ef1α and EIF1A were relatively universally available reference gene of F. crenata leave from the different season and stresses. Third, we found abiotic stress-dependent expression of genes. Consequently, we suggested transcriptome analysis was a promising approach for stress diagnosis of beech tree in field.

Research Products

• [Journal Article] エアロゾル・オゾン等による植物影響-増加する越境大気汚染から森林を守る- (2010)
  • Author(s): 斎藤秀之, 伊森允一, 青山千穂, 渡辺誠, シンポジウム
  • Journal Title: に参加して, 北方林業 (掲載予定)
• [Journal Article] Stress diagnosis of beech tree by genomic ecophysiology - A perspective. (2009)
  • Author(s): Saito H
  • Journal Title: Ecology and Silviculture of Beech Pages: 178-180
• [Journal Article] ブナの葉で発現する遺伝子の完全長cDNAクローンの網羅的収集 (2009)
  • Author(s): 斎藤秀之
  • Journal Title: 北海道の林木育種 51(2) Pages: 1-4
  • NAID: 40016523642
• [Journal Article] Stress diagnosis of beech tree by genomic ecolikysiology-A Perspective (2009)
  • Author(s): Saito H
  • Journal Title: Proceedings of the 8^<th> IUFRO Internationl Beech Symposium "Ecology and Silviculture of Beech" held in 8-13 September 2008, Nanae, Hokkaido, Japan (Eds. Terazawa K. Madsen P. and Sagheb-Talebi K.) 8 Pages: 178-180
• [Journal Article] ブナの葉で発現する遺伝子の完全長cDNAクローンの網羅的収集 (2009)
  • Author(s): 斎藤秀之
  • Journal Title: 北海道の林木育種 2 Pages: 1-4
  • NAID: 40016523642
  • Peer Reviewed
• [Presentation] ブナ花成の光周性に関わる転写因子CONSTANS様遺伝子群の単離と発現特性～どうして毎年花を咲かさないか? (2010)
  • Author(s): 斎藤秀之・大宮泰徳・高田直樹
  • Organizer: 日本森林学会
  • Place of Presentation: つくば市, 筑波大学
  • Year and Date: 2010-04-04
• [Presentation] ポストゲノム時代における新たな森林研究の展開(テーマ別シンポジウム) (2010)
  • Author(s): 高田克彦・斎藤秀之・渡辺敦史・高橋誠
  • Organizer: 日本森林学会
  • Place of Presentation: つくば市, 筑波大学
  • Year and Date: 2010-04-04
• [Presentation] ブナ花成関連遺伝子の単離と解析 (2010)
  • Author(s): 大宮泰徳・松田修一・高田直樹・上村松生・斎藤秀之・赤田辰治
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本市, 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] 樹木の機能遺伝子研究の現状と今後の利用にむけて(テーマ別シンポジューム) (2009)
  • Author(s): 斎藤秀之・楠城時彦
  • Organizer: 日本森林学会
  • Place of Presentation: 京都大学(京都)
  • Year and Date: 2009-03-26
• [Presentation] ブナの葉の遺伝子発現パターンを指標にした生理生態評価-林木の生理生態評価における既存手法の限界、ゲノム情報への期待- (2009)
  • Author(s): 斎藤秀之
  • Organizer: 日本森林学会
  • Place of Presentation: 京都大学(京都)
  • Year and Date: 2009-03-26
• [Presentation] Stress diagnosis of beech tree by genomic ecophysiology - A perspective. (2008)
  • Author(s): Saito H.
  • Organizer: 8th IUFRO Internationl Beech Symposium " Ecology and Silviculture of Beech"
  • Place of Presentation: Onuma International Seminar House, Nanae, Hokkaido
• [Presentation] Stress diagnosis of beech tree by genomic ecophysiology-A perspective (2008)
  • Author(s): Saito H
  • Organizer: 8^<th> IUFRO Internationi Beech Symposium "Ecology and Silviculture of Beech"
  • Place of Presentation: Onuma International Seminar House (Nanae, Hokkaido)