Gross production and consumption measurement of N2O and CH4 by using stable isotope tracer technique

• Project/Area Number: 20780113
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Forest science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: KOBA Keisuke Tokyo University of Agriculture and Technology, 大学院・農学研究院, 准教授 (90311745)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 窒素同位体 / メタン / 一酸化二窒素 / 同位体トレーサー / 15N / 180 / 18O

Research Abstract

Soil can act as a sink and a source of methane and nitrous oxide. To distinguish between production and consumption of these gases, I attempted to develop a new, handy analytical method for isotope tracer. Dual labeled N2O is now produced via 15N- and 18O-labelled nitrogen compound, and it is possible to separate N2O production and consumption for a single soil sample.

Research Products

• [Journal Article] Nitrogen deposition an dofrest nitrogen cycling along an urban-rural transect in southern China (2011)
  • Author(s): Fang Y, Yoh M, Koba K(他7名)
  • Journal Title: Global Change Biology 17 Pages: 872-885
  • Peer Reviewed
• [Journal Article] Analytical Techniques for quantifying 15N/14N of nitrate, nitrite, total dissolved nitrogen and ammonium in environmental samples using a gas chromatograph equipped with a quadrupole mass spectrometer (2011)
  • Author(s): Isobe K, ら(他11名中11番目)
  • Journal Title: Microbes and Environments 26 Pages: 46-53
  • Peer Reviewed
• [Journal Article] Anthropogenic imprints on nitrogen and oxygen isotopic composition of precipitation nitrate in a nitrogen-polluted city in southern China (2011)
  • Author(s): Fang Y, Koba K(他6名)
  • Journal Title: Atmospheric Chemistry and Physics 11 Pages: 1313-1325
  • Peer Reviewed
• [Journal Article] Analytical Techniques for Quantifying 15N/14N of Nitrate, Nitrite, Total Dissolved Nitrogen and Ammonium in Environmental Samples Using a Gas Chromatograph Equipped with a Quadrupole Mass Spectrometer (2011)
  • Author(s): Isobe, et al.
  • Journal Title: Microbes and Environments Volume: 26 Pages: 46-53
  • Peer Reviewed
• [Journal Article] A simple and rapid GC/MS method for the simultaneous determination of gaseous metabolites (2011)
  • Author(s): Isobe, et al.
  • Journal Title: Journal of Microbiological Methods Volume: 84 Pages: 46-51
  • Peer Reviewed
• [Journal Article] Contribution of atmospheric nitrate to stream-water nitrate in Japanese coniferous forest revealed by the oxygen isotope ratio of nitrate (2010)
  • Author(s): Tobari Y, Koba, K(他7名)
  • Journal Title: Rapid Communications in Mass Spectrometry 24 Pages: 1281-1286
  • Peer Reviewed
• [Journal Article] The natural abundance of 15N in plant and soil-available N indicates a shift of main plant N resources to NO3 to NH4 along the N leaching gradient (2010)
  • Author(s): Takebayashi Y, Koba K(他3名)
  • Journal Title: Rapid Communications in Mass Spectrometry 24 Pages: 1001-1008
  • Peer Reviewed
• [Journal Article] The natural abundance of 15N in plant and soil-avalable N indicates a shift of main plant N resources to NO3- from NH4+ along the N leaching gradient (2010)
  • Author(s): Takebayashi Y, Koba K, Sasaki Y, Fang Y, Yoh M.
  • Journal Title: Rapid Communications in Mass Spectrometry 24 Pages: 1001-1008
  • Peer Reviewed
• [Journal Article] Contribution of atmospheric nitrate to stream-water nitrate in Japanese coniferous forests revealed by the oxygen isotope ratio of nitrate (2010)
  • Author(s): Tobari Y, Koba K, Fukushina K, Tokuchi N, Ohte N, Tateno R, Toyoda S, Yoshioka T, Yoshida N.
  • Journal Title: Rapid Communications in Mass Spectrometry 24 Pages: 1281-1286
  • Peer Reviewed
• [Journal Article] Biogeochemistry of nitrous oxide in groundwater in a forested ecosystem elucidated by nitrous oxide isotopomer measurements (2009)
  • Author(s): KobaK. 他12名
  • Journal Title: Geochimica et Cosmochimica Acta 73 Pages: 3115-3133
  • Peer Reviewed
• [Presentation] 自然安定同位体比を用いた一酸化二窒素生成プロセスの解析 (2009)
  • Author(s): 木庭啓介
  • Organizer: 日本微生物生態学会
• [Presentation] Nitrogen cycling in Lake Kizaki elucidated by isotopic analysis (2008)
  • Author(s): Y.Sasaki, K.Koba(他7名)
  • Organizer: International Symposium on Isotopomers
• [Remarks] ホームページ等
  • URL: http://www.tuat.ac.jp/~keikoba/
• [Remarks]
  • URL: http://www.that.ac.jp/~keikoba/