Identification of fresh and seawater specific molecular markers in teleosts

• Project/Area Number: 20780147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: St.Marianna University School of Medicine
• Principal Investigator: HIROI Junya St.Marianna University School of Medicine, 医学部, 准教授 (20350598)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 魚類 / 環境適応 / 浸透圧調節 / 淡水 / 海水 / 塩類細胞 / イオントランスポーター / イオン輸送体

Research Abstract

Euryhaline fish can live in both fresh water and seawater by means of specialized "mitochondrion-rich cells (MRCs)". Although MRCs are considered to be involved in both ion uptake in fresh water and ion secretion in seawater, the ion-uptake mechanism has been controversial for over 30 years. In this study, we identified a novel freshwater-type ion transporter in tilapia MRCs and proposed a new ion uptake model. We also found that ion uptake mechanisms vary among fish species. These findings are opening a new window for the study on fish osmo- and iono-regulation.

Research Products

• [Journal Article] Morphological and functional classification of ion-absorbing mitochondriarich cells in the gills of Mozambique tilapia (2009)
  • Author(s): Inokuchi M, Hiroi J, Watanabe S, Hwang PP, Kaneko T
  • Journal Title: Journal of Experimental Biolog 212 Pages: 1003-1010
  • Peer Reviewed
• [Journal Article] Role of SLC12A10. 2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio) (2009)
  • Author(s): Wang YF, Tseng YC, Yan JJ, Hiroi J, Hwang PP
  • Journal Title: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 296
  • Peer Reviewed
• [Journal Article] Different hatching strategies in embryos of two species, pacific herring Clupea pallasii and Japanese anchovy Engraulis japonicus, that belong to the same order Clupeiformes, and their environmental adaptation (2009)
  • Author(s): Kawaguchi M, Fujita H, Yoshizaki N, Hiroi J, Okouchi H, Nagakura Y, Noda T, Watanabe S, Katayama S, Iwamuro S, Nishida M, Iuchi I, Yasumasu S
  • Journal Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 312 Pages: 95-107
  • Peer Reviewed
• [Journal Article] Morphological and functional classification of ion-absorbing mitochondria-rich cells inthe gills of Mozambique tilapia. (2009)
  • Author(s): Inokuchi, M., et al.
  • Journal Title: Journal of Experimental Biology 212-7 Pages: 1003-1010
  • Peer Reviewed
• [Journal Article] Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism inzebrafish (Danio rerio). (2009)
  • Author(s): Wang, Y. F., et al.
  • Journal Title: American Journal of Physiology -Regulatory, lntegrativeand Comparative Physiology 296-5
  • Peer Reviewed
• [Journal Article] Different hatching strategies in embryos of two species, pacific herring Clupea pallasii and Japanese anchovy Engraulis japonicus, that belong to the same order Clupeiformes, and their environmental adaptation (2009)
  • Author(s): Kawaguchi, M., et.al.
  • Journal Title: Journal of Experimental Zoology- Part B 312B・2 Pages: 95-107
  • Peer Reviewed
• [Journal Article] Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinity (2008)
  • Author(s): Inokuchi M, Hiroi J, Watanabe S, Lee KM, Kaneko T
  • Journal Title: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology 151 Pages: 151-158
  • Peer Reviewed
• [Journal Article] Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish (2008)
  • Author(s): Hiroi J, Yasumasu S, McCormick SD, Hwang PP, Kaneko T
  • Journal Title: Journal of Experimental Biology 211 Pages: 2584-2599
  • Peer Reviewed
• [Journal Article] Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii-environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene (2008)
  • Author(s): Kawaguchi M, Nakagawa M, Noda T, Yoshizaki N, Hiroi J, Nishida M, Iuchi I, Yasumasu S
  • Journal Title: FEBS Journal 275 Pages: 2884-2898
  • Peer Reviewed
• [Journal Article] Evidence for an apical Na- Cl cotransporter involved in ion uptake in a teleost fish (2008)
  • Author(s): Hiroi, J., et.al.
  • Journal Title: Journal of Experimental Biology 211・16 Pages: 2584-2599
  • Peer Reviewed
• [Journal Article] Gene expression and morphological localization ofNHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide ranee of salinities (2008)
  • Author(s): Inokuchi, M., et.al.
  • Journal Title: Comparative Biochemistry and Physiology-Part A 151・2 Pages: 151-158
  • Peer Reviewed
• [Journal Article] Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii-environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene (2008)
  • Author(s): Kawaguchi, M., et.al.
  • Journal Title: FEBS Journal 275・11 Pages: 2884-2898
  • Peer Reviewed
• [Presentation] Evidence for an apical Na-Cl cotransporter involved in ion uptake in tilapia embryos (2008)
  • Author(s): Hiroi J
  • Organizer: 8th International Congress on the Biology of Fish
  • Place of Presentation: Portland, OR, USA
  • Year and Date: 2008-07-30
• [Presentation] Evidence for an apical Na- Cl cotransporter involved in ion uptake in tilapia embryos (2008)
  • Author(s): Hiroi, J
  • Organizer: 8th International Congress on the Biology of Fish
  • Place of Presentation: Portland, OR, USA
  • Year and Date: 2008-07-30
• [Presentation] 魚類の「塩類細胞」に局在するcation-chloride cotransporter (2008)
  • Author(s): 廣井準也
  • Organizer: 第3回トランスポーター研究会年会
  • Place of Presentation: 京都
  • Year and Date: 2008-06-08
• [Book] 稚魚学-多様な生理生態を探る (2008)
  • Author(s): 廣井準也
  • Publisher: 生物研究社
• [Book] Fish Larval Physiology(eds. R.N.Finn and B.G.Kapoor) (2008)
  • Author(s): Kaneko T, Hiroi J
  • Publisher: Science Publishers, Enfield
• [Book] 魚の浸透圧調節の謎に挑む. 稚魚学-多様な生理生態を探る(田申克編) (2008)
  • Author(s): 廣井準也(分担執筆)
  • Publisher: 生物研究社
• [Book] Osmo-and Ionoregulation. In Fish Larval Physiology (ed. R. N. Finn and B. G. Kapoor) (2008)
  • Author(s): Kaneko, T. and Hiroi, J.(分担執筆)
  • Publisher: Scientific Publishers