The bacterial controls using antimicrobial peptides from complement components of fish

Research Project

Project/Area Number	20780148
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	General fisheries
Research Institution	Fukuoka Women's Junior College
Principal Investigator	MUTSURO Junichi Fukuoka Women's Junior College, 食物栄養科, 講師 (40399100)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	魚病 / 生体防御 / 補体 / 抗菌ペプチド / 食品保存
Research Abstract	Our results revealed that the synthetic peptide corresponding to partial amino acid sequence (KVFLECCNLMKTHKNMKTE) of C3a region of carp C3 isotype (C3-Q1) was antibacterial, and acted on Gram-negative bacteria, Escherichia coli. (XL1-Blue mrf and O157:H7 (VT1 and VT2)) and S.Enteritidis. We successfully enhanced antibacterial activity, by partially replacing amino acids of above-mentioned sequence. However, it was found that enhancement of antibacterial activity led to high cytotoxic activity. Hereafter, we aim to create antimicrobial peptides with low side effects on the basis of observation in this study.

Report

(4 results)