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Molecular mechanisms on migration of slow muscle cells in fish myogenesis

Research Project

Project/Area Number 20780152
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

KINOSHITA Shigeharu  The University of Tokyo, 大学院・農学生命科学研究科, 助教 (40401179)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords筋発生 / 魚類 / 遅筋 / 細胞移動 / 水産学 / 発生・分化 / トラフグ / 小型魚類
Research Abstract

Lateral migration of adaxial cell-derived slow muscle cells is crucial in fish myogenesis. However, detailed molecular mechanisms responsible for the migration are largely unknown. In this study, we established transgenic fish which expresses fluorescent protein in migrating and not migrating slow muscle cells. By using the transgenic fish, we revealed that distinct mechanisms are involved in gene expression specifically to migrating and not migrating slow muscle cells.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (26 results)

All 2010 2009 2008 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (23 results) Remarks (1 results)

  • [Journal Article] Early development of medaka Oryzias latipes muscles as revealed by transgenic approaches using embryonic and larval types of myosin heavy chain genes.2010

    • Author(s)
      Yosuke Ono, Shigeharu Kinoshita, Daisuke Ikeda, Shugo Watabe
    • Journal Title

      Developmental Dynamics 239

      Pages: 1807-1817

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Early development of medaka Oryzias latipes muscles as revealed by transgenic approaches using embryonic and larval types of myosin heavy chain genes2010

    • Author(s)
      Yosuke Ono, Shigeharu Kinoshtia, Daisuke Ikeda, Shugo Watabe
    • Journal Title

      Developmental Dynamics (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 遅筋/心筋型魚類ミオシン重鎖遺伝子トラフグMYHM5の組織特異的発現に関与するプロモーターの解析2010

    • Author(s)
      木下滋晴・Md. Asaduzzaman・小野陽介・D.B. Akolkar・浅川修一・渡部終五
    • Organizer
      2010年度日本水産学会春季大会
    • Place of Presentation
      藤沢
    • Year and Date
      2010-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] 遅筋/心筋型魚類ミオシン重鎖遺伝子トラフグMYH_<M5>の組織特異的発現に関与するプロモーターの解析2010

    • Author(s)
      木下滋晴・Md.Asaduzzaman・小野陽介・Dadasaheb B.Akolkar・浅川修一・渡部終五
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      藤沢
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] ミオシン重鎖アイソフォームを用いた筋特異的プロモーターの解析2010

    • Author(s)
      木下滋晴・Md. Asaduzzaman・小野陽介・D.B. Akolkar・浅川修一・渡部終五
    • Organizer
      2010年生体運動研究合同班会議
    • Place of Presentation
      東京
    • Year and Date
      2010-01-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 魚類の表層遅筋および心筋に特異的な遺伝子発現に必要な転写制御領域の解析2009

    • Author(s)
      木下滋晴・Md. Asaduzzaman・小野陽介・D.B. Akolkar・浅川修一・渡部終五
    • Organizer
      2009年度日本水産学会秋季大会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-10-01
    • Related Report
      2009 Final Research Report
  • [Presentation] 魚類の表層遅筋および心筋に特異的な遺伝子発現に必要な転写制御領域の解析2009

    • Author(s)
      木下滋晴・Md.Asaduzzaman・小野陽介・Dadasaheb B.Akolkar・浅川修一・渡部終五
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      岩手
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification functional analysis of the promoter region in the torafugu myosin heavy chain gene, MYHM5, involved in cardiac and superficial slow muscle specific expressions.2009

    • Author(s)
      S. Kinoshita, Y. Sugano, Y. Ono, S. Watabe
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Roma, Italy
    • Year and Date
      2009-07-18
    • Related Report
      2009 Final Research Report
  • [Presentation] The spatio-temporal expression of sarcomeric myosin heavy chain genes during muscle development of medaka Oryzias latipes are regulated by their 5'-flanking region as revealed by transgenic constructs.2009

    • Author(s)
      Y. Ono, S. Kinoshita, S. Watabe
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Roma, Italy
    • Year and Date
      2009-07-18
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification functional analysis of the promoter region in the torafugu myosin heavy chain gene, MYH_<M5>, involved in cardiac and superficial slow muscle specific expressions2009

    • Author(s)
      Shigeharu Kinoshita, Yuya Sugano, Yosuke Ono, Shugo Watabe
    • Organizer
      6^<th> European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Roma Itaria
    • Year and Date
      2009-07-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] The spatio-temporal expression of sarcomeric myosin heavy chain genes during muscle development of medaka Oryzias latipes are regulated by their 5'-flanking region as revealed by transgenic constructs2009

    • Author(s)
      小野陽介・木下滋晴・渡部終五
    • Organizer
      6^<th> European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Roma Itaria
    • Year and Date
      2009-07-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Expression patterns of multiple myosin heavy chain genes identify tissue-specific fibre types in adult skeletal muscles of torafugu Takifugu rubripes.2009

    • Author(s)
      D.B. Akolkar, S. Kinoshita, Y. Ono, S. Watabe
    • Organizer
      Annual Main Meeting 2009 of the Society for Experimental Biology
    • Place of Presentation
      Glasgow, UK
    • Year and Date
      2009-06-28
    • Related Report
      2009 Final Research Report
  • [Presentation] メダカ胚型ミオシン重鎖遺伝子の発現を制御する上流領域の解析2009

    • Author(s)
      小野陽介・木下滋晴・渡部終五
    • Organizer
      2009年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 魚類の心筋および遅筋に特異的なプロモーターの単離と機能解析2009

    • Author(s)
      木下滋晴・菅野雄耶・小野陽介・渡部終五
    • Organizer
      2009年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] メダカ胚型ミオシン重鎖遺伝子の発現を制御する上流領域の解析2009

    • Author(s)
      小野陽介・木下滋晴・渡部終五
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 魚類の心筋および遅筋に特異的なプロモーターの単離と機能解析2009

    • Author(s)
      木下滋晴・菅野雄耶・小野陽介・渡部終五
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] メダカ胚発生における骨格筋型ミオシン重鎖の発現制御機構2009

    • Author(s)
      小野陽介・木下滋晴・渡部終五
    • Organizer
      生体運動研究合同班会議2009
    • Place of Presentation
      東京
    • Year and Date
      2009-01-10
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] A 5'-flanking region of the myosin heavy chain gene from torafugu (Takifugu rubripes) promotes developmental stage-specific transcription.2008

    • Author(s)
      L. Yasmin, S. Kinoshita, D. Ikeda, Y. Ono, D.B. Akolkar, S. Watabe
    • Organizer
      WFC2008 5th World Fisheries Congress
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] The regulation in the expression of sarcomeric myosin heavy chain genes during muscle development in medaka Oryzias latipes.2008

    • Author(s)
      Y. Ono, S. Kinoshita, D. Ikeda, S. Watabe
    • Organizer
      WFC2008 5th World Fisheries Congress
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] Dominant sarcomeric myosin heavy chain genes expressed in adult fast, slow and cardiac muscles in torafugu Takifugu rubripes.2008

    • Author(s)
      D.B. Akolkar, S. Kinoshita, L. Yasmin, Y. Ono, D. Ikeda, S. Watabe
    • Organizer
      WFC2008 5th World Fisheries Congress
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] Fiber type-specific transcriptional regulation in the expression of fish myosin heavy chain genes.2008

    • Author(s)
      S. Kinoshita, Y. Ono, D. Ikeda, S. Watabe
    • Organizer
      WFC2008 5th World Fisheries Congress
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] Fiber Type-specific Transcriptional Regulation in the Expression of Fish Myosin Heavy Chain Genes2008

    • Author(s)
      S. Kinoshita, Y. Ono, D. Ikeda, and S. Watabe
    • Organizer
      WFC2008 5^<th> World Fisheries Congress
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] The regulation in the expression of sarcomeric myosin heavy chain genes during muscle development in medaka Oryzias latipes2008

    • Author(s)
      Y. Ono, S. Kinoshita, D. Ikeda, and S. Watabe
    • Organizer
      WFC2008 5^<th> World Fisheries Congress
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] メダカの筋発生におけるミオシン重鎖遺伝子MYHemb1の発現制御と機能2008

    • Author(s)
      小野陽介・木下滋晴・渡部終五
    • Organizer
      第5回東京大学生命科学シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2008-04-19
    • Related Report
      2009 Final Research Report
  • [Presentation] メダカの筋発生におけるミオシン重鎖遺伝子MYHemb1の発現制御と機能2008

    • Author(s)
      小野陽介・木下滋晴・渡部終五
    • Organizer
      第5回東京大学の生命科学シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2008-04-19
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.suikou.fs.a.u-tokyo.ac.jp/index.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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