Clarification of the Toll-like receptor 2-mediated anti-allergic function of fermented milk-derived microorganisms

• Project/Area Number: 20780191
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: Shinshu University
• Principal Investigator: KAWAHARA Takeshi Shinshu University, 農学研究科, 助教 (30345764)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: マスト細胞 / アレルギー / 乳酸菌 / 脱顆粒反応 / FcεRI / Tollと様受容体2 / TLR2 / I型アレルギー

Research Abstract

This study investigated the suppressive function of fermented milk-derived microorganisms that work as the ligand of Toll-like receptor 2. The strong suppressive effect of lactic acid bacterial strain (Lactobacillus reuteri NBRC15892) on both degranulation and subsequent late-phase reactions (induction of proinflammatory cytokines and production of lipid mediators) of mast cells are elucidated by the screening and investigation using this strain.

Research Products

• [Journal Article] Inhibitory effect of heat-killed Lactobacillus strain on immunoglobulin E-mediated degranulation and late-phase immune reactions of mouse bone marrow-derived mast cells. (2010)
  • Author(s): Kawahara, T
  • Journal Title: Animal Science Journal (in press)
  • NAID: 10027890281
  • Peer Reviewed
• [Journal Article] Bacteriocinogenic lactic acid bacteria Lactobacillus curvatus strain Y108 isolated from Nozawana-zuke pickles (2010)
  • Author(s): Kawahara, T., Iida, A., Toyama, Y., Fukuda, K.
  • Journal Title: Food Science and Technology Research 16 Pages: 253-262
  • Peer Reviewed
• [Journal Article] Inhibitory effect of heat-killed Lactobacillus strain on immunoglobulin E-mediated degranulation and late-phase immune reactions of mouse bone marrow-derived mast cells. (2010)
  • Author(s): Kawahara, T.
  • Journal Title: Animal Science Journal ((in press))
  • NAID: 10027890281
  • Peer Reviewed
• [Journal Article] Bacteriocinogenic lactic acid bacteria Lactobacillus curvatus strain Y108 isolated from Nozawana-zuke pickles (2010)
  • Author(s): Kawahara, T., Iida, A., Toyama, Y., Fukuda, K.
  • Journal Title: Food Science and Technology Research ((in press))
  • Peer Reviewed
• [Journal Article] Anti-Allergic Effect of a Hot-Water Extract of Quince (Cydonia oblonga) (2009)
  • Author(s): Shinomiya, F., Hamauzu, Y., Kawahara T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry 73 Pages: 1773-1778
  • NAID: 10027544392
  • Peer Reviewed
• [Journal Article] Anti-Allergic Effect of a Hot-Water Extract of Quince(Cydonia oblonga) (2009)
  • Author(s): Shinomiya, F., Hamauzu, Y.., and Kawahara T
  • Journal Title: Bioscience, Biotechnology, and Biochemistry (in press)
  • NAID: 10027544392
  • Peer Reviewed
• [Journal Article] Inhibitory effect of electrolyzed reduced water on tumor angiogenesis (2008)
  • Author(s): Ye, J., Li, Y., Hamasaki, T., Nakamichi, N., Komatsu, T., Kashiwagi, T., Teruya, K., Nishikawa, R., Kawahara, T., (他9名、9番目)
  • Journal Title: Biological & Pharmaceutical Bulletin 31 Pages: 19-26
  • NAID: 110006569968
  • Peer Reviewed
• [Journal Article] Inhibitory effect of electrolyzed wayer on tumor angibgenesis (2008)
  • Author(s): Ye, J, Li, Y, Hamasaki, T., Nakamichi, N., Komatsu, T, kashiwagi, T, teruya, K., Nishikawa, R., Kawahara. T, 他9名
  • Journal Title: Biological and Pharmaceutical Bulletin 31 Pages: 19-26
  • Peer Reviewed
• [Presentation] 脱顆粒反応抑制性乳酸菌のTLR2依存性と活性画分の作用に関する特性付け (2010)
  • Author(s): 河原岳志, 戸田圭亮
  • Organizer: 日本畜産学会第110回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 脱顆粒反応抑制性乳酸菌の機能解析におけるマウスマスト細胞ハイブリドーマの利用性 (2010)
  • Author(s): 山崎浩史, 河原岳志
  • Organizer: 日本畜産学会第110回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 野沢菜漬由来乳酸菌のマウス骨髄由来マスト細胞に対する脱顆粒反応抑制効果 (2010)
  • Author(s): 戸田圭亮, 河原岳志
  • Organizer: 日本畜産学会第110回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 脱顆粒反応抑制性乳酸菌のTLR2依存性と活性画分の作用に関する特性付け (2010)
  • Author(s): 河原岳志、戸田圭亮
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 明治大学(東京都)
  • Year and Date: 2010-03-29
• [Presentation] 脱顆粒反応抑制性乳酸菌の機能解析におけるマウスマスト細胞ハイブリドーマの利用性 (2010)
  • Author(s): 山崎浩史、河原岳志
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 明治大学(東京都)
  • Year and Date: 2010-03-29
• [Presentation] 野沢菜漬由来乳酸菌のマウス骨髄由来マスト細胞に対する脱顆粒反応抑制効果 (2010)
  • Author(s): 戸田圭亮、河原岳志
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 明治大学(東京都)
  • Year and Date: 2010-03-29
• [Presentation] 脱顆粒反応抑制性乳酸菌のマウス骨髄由来マスト細胞遅延相応答に対する抑制効果 (2009)
  • Author(s): 河原岳志, 大谷元
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 神奈川
  • Year and Date: 2009-03-29
• [Presentation] 脱顆粒反応抑制乳酸菌のマウス骨髄由来マスト細胞遅延相応答に対する抑制効 (2009)
  • Author(s): 河原岳志、大谷元
  • Organizer: 日本畜産学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-03-27
• [Presentation] 発酵乳由来微生物のマウスマスト細胞に及ぼす影響の評価 (2009)
  • Author(s): 河原岳志, 大谷元
  • Organizer: 平成20年度北信越畜産学会
  • Place of Presentation: 石川
• [Presentation] 発酵乳由来微生物のマウスマスト細胞に及ぼす影響の評価 (2008)
  • Author(s): 河原岳志、大谷元
  • Organizer: 北信越畜産学会
  • Place of Presentation: 金沢エクセルホテル東急
  • Year and Date: 2008-11-07
• [Presentation] 発酵乳由来微生物のマウスマスト細胞に及ぼす影響の評価 (2008)
  • Author(s): 河原岳志, 大谷元
  • Organizer: 平成20年度日本酪農科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-29
• [Presentation] 発酵乳由来微生物のマウスマスト細胞に及ぼす影響の評価 (2008)
  • Author(s): 河原岳志、大谷元
  • Organizer: 日本酪農科学会
  • Place of Presentation: 武蔵野スイングホール
  • Year and Date: 2008-08-29