| Project/Area Number |
20780195
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Kitasato University |
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Principal Investigator |
ISHIKAWA Shin-ichi Kitasato University, 食産業学部, 准教授 (00327462)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 畜産食品 / ホスビチン / 抗酸化作用 / 抗炎症作用 / 免疫賦活化作用 / 畜産産品 / 抗酸化 / ペプチド / 卵黄 / 免疫賦活 / ホスホセリン / インターロイキン-8 / 抗がん作用 |
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| Research Abstract |
Oxidative stress has been implicated in the mucosal inflammation of the gastrointestinal tract. Several studies have described the antioxidant activity of proteins from several animal and plant sources. The antioxidant activity of peptides generated from the hydrolysis of various proteins, such as egg white protein, egg yolk protein, soy protein, and milk casein has been reported. Recent studies have described the antioxidant activity of egg yolk protein phosvitin and phosvitin phosphopeptides (PPP). An inflammatory chemokine, interleukin-8 (IL-8), is induced by oxidative stress, and the antioxidants have been shown to inhibit IL-8 expression. Hydrogen peroxide is known to increase IL-8 production in human epithelial cell lines. We investigated the effect a chemically synthesized poly-phosphoserine as a moldel of PPP on the production of IL-8 induced by hydrogen peroxide in intestinal epithelial Caco-2 cells. The chemokine production was measured by enzyme-linked immunosorbent assay (ELISA). We found that the synthesized poly-phosphoserine significantly inhibited the hydrogen peroxide-induced IL-8 production in Caco-2 cells. Moreover, the inhibition was dose-dependent. The results suggested that the phosphopeptides have the potential to attenuate intestinal inflammation by oxidative stress.
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