Investigation of the function of mitochondria in the oocytes during oocyte maturation in pigs.

• Project/Area Number: 20780198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: Tohoku University
• Principal Investigator: YOKOO Masaki Tohoku University, 生物資源科学部, 准教授 (10396541)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 繁殖 / ミトコンドリア機能 / ブタ / 卵子 / ミトコンドリア / 呼吸 / 卵成熟

Research Abstract

I investigated that the oxygen consumption of single porcine COCs and oocytes and the function of mitochondria in the oocytes during oocyte maturation. From this study, it is demonstrated that the oocytes with high oxygen consumption and ATP levels showed higher competence of oocyte maturation. Furthermore, these findings suggest that mitochondrial reorganization may be partly related to the oxygen consumption and consequently the quality of porcine oocytes.

Research Products

• [Journal Article] Induction of oocyte maturation by hyaluronan-CD44 interaction in pigs. (2010)
  • Author(s): Masaki Yokoo, Naoko Kimura, Eimei Sato
  • Journal Title: Journal of Reproduction and Development 56 Pages: 15-19
  • NAID: 10026323088
• [Journal Article] Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos. (2010)
  • Author(s): Satoshi Sugimura, Ken-ichi Yamanaka, Manabu Kawahara, Takuya Wakai, Masaki Yokoo, Eimei Sato
  • Journal Title: Animal Science Journal 81 Pages: 48-57
  • NAID: 10027888500
• [Journal Article] Morphological evaluation and measurement of the respiration activity of cumulus-oocyte complexes to assess oocyte quality. (2010)
  • Author(s): Haruo Murakawa, Nobuya Aono, TakayukiTanaka, Hiroyuki Kikuchi, Hidemune Yoshida, Hiroaki Yoshida, Masaki Yokoo, Hiroyuki Abe
  • Journal Title: Journal of Mammalian Ova Research 26 Pages: 32-41
  • NAID: 10024964622
• [Journal Article] Induction of oocyte maturation by hyaluronan-CD44 interaction in pigs. (2010)
  • Author(s): Yokoo, et al.
  • Journal Title: Journal of Reproduction and Development 56 Pages: 15-19
  • NAID: 10026323088
• [Journal Article] Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos. (2010)
  • Author(s): Sugimura, et al.
  • Journal Title: Animal Science Journal 81 Pages: 48-57
  • Peer Reviewed
• [Journal Article] Genomic screening for genes silenced by DNA methylation revealed an association between RASD1 inactivation and dexamethasone resistance in multiple myeloma. (2009)
  • Author(s): Nojima M, Maruyama R, Yasui H, Suzuki H, Maruyama Y, Tarasawa I, Sasaki Y, Asaoku H, Sakai H, Hayashi T, Mori M, Imai K, Tokino T, Ishida T, Toyota M, Shinomura Y
  • Journal Title: Clin Cancer Res 15(13) Pages: 4356-64
  • Peer Reviewed
• [Journal Article] Evaluation of human cumulus-oocyte complexes during in vitro maturation. (2009)
  • Author(s): Murakawa, et al.
  • Journal Title: Journal of Mammalian Ova Research 26 Pages: 32-41
  • Peer Reviewed
• [Journal Article] Influence of hyaluronan accumulation during cumulus expansion on porcine oocyte maturation in vitro. (2008)
  • Author(s): Masaki Yokoo, Naoko Kimura, Hiroyuki Abe, Eimei Sato
  • Journal Title: Zygote 16 Pages: 309-314
• [Journal Article] Multiple analysis of respiratory activity in the identical oocytes by applying scanning electrochemical microscopy. (2008)
  • Author(s): Masaki Yokoo, Takahiro Ito-Sasaki, Hitoshi Shiku, Tomokazu Matsue, Hiroyuki Abe
  • Journal Title: Transactions of the Materials Research Society of Japan 33 Pages: 759-762
  • NAID: 130007809492
• [Journal Article] Influence of hyaluronan accumulation during cumulus expansion on porcine oocyte maturation in vitro (2008)
  • Author(s): Yokoo et.al.
  • Journal Title: Zygote 16 Pages: 309-314
  • Peer Reviewed
• [Journal Article] Multiple analysis of respiratory activity in the identical oocytes by applying scanning electrochemical microscopy (2008)
  • Author(s): Yokoo et.al.
  • Journal Title: Transactions of the Materials Research Society of Japan 33 Pages: 759-762
  • NAID: 130007809492
  • Peer Reviewed
• [Presentation] Embryo development after vitrification-thawing can be predicted by assessment of oxygen consumption. (2009)
  • Author(s): Tanaka, et al.
  • Organizer: The international ovarian conference 2009
  • Place of Presentation: Tokyo
  • Year and Date: 2009-12-05
• [Presentation] Embryo development after vitrification-thawing can be predicted by assessment of oxygen consumption.
  • Author(s): Takayuki Tanaka, Nobuya Aono, Yuki Iwasa, Tomoko Kamono, Hiroyuki Kikuchi, Asami Suzuki, Haruo Murakawa, Hidemune Yoshida, Masaki Yokoo, Hiroyuki Abe, Hiroaki Yoshida
  • Organizer: The international ovarian conference 2009
  • Place of Presentation: Tokyo
• [Book] カラーアトラス不妊診療のための卵子学 (2010)
  • Author(s): 佐藤英明, 星野由美, 横尾正樹
  • Total Pages: 208
  • Publisher: 医歯薬出版