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Determination of paternally methylated imprinted regions in mouse genome

Research Project

Project/Area Number 20780201
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionSaga University

Principal Investigator

KAWAHARA Manabu  Saga University, 農学部, 准教授 (70468700)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsゲノムインプリンティング / 核移植 / バイオインフォマティクス / 胎盤形成 / マイクロアレイ / 哺乳類 / 卵子ゲノム
Research Abstract

Parental genome functions in ontogeny are determined by interactions among transcripts from the maternal and paternal genomes, which contain many genes whose expression is strictly dependent on their parental origin as a result of genomic imprinting. Comprehensive recognition of the interactions between parental genomes is important for understanding genomic imprinting in mammalian development. The bi-maternal embryos were produced by the construction of oocytes from fully grown oocytes and nongrowing oocytes that contain double deletions in the H19 differentially methylated region (DMR) and the Dlk1-Dio3 intergenic germline-derived DMR. Through a sequence of studies, we demonstrated that analyses of ng/fg bi-maternal conceptuses helped in elucidating the roles of paternally methylated imprinted genes in mouse ontogeny.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (23 results)

All 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (6 results) Remarks (5 results)

  • [Journal Article] Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryos2010

    • Author(s)
      杉村智志, 横尾正樹, 山中謙一, 川原学, 森安悟, 若井拓哉, 永井卓, 阿部宏之, 佐藤英明
    • Journal Title

      Cellular Reprogramming (印刷中)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Longevity in mice without a father2010

    • Author(s)
      川原学, 河野友宏
    • Journal Title

      Human Reproduction 25巻

      Pages: 457-461

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of insulin-like growth Factor 2 in angiogenic factor transcription in bi-maternal mouse conceptuses2010

    • Author(s)
      川原学, Qiong Wu, 河野友宏
    • Journal Title

      Journal of Reproduction and Development 56巻

      Pages: 79-85

    • NAID

      10026323565

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos2010

    • Author(s)
      杉村智志, 山中謙一, 川原学, 若井拓哉, 横尾正樹, 佐藤英明
    • Journal Title

      Animal Science Journal 81巻

      Pages: 48-57

    • NAID

      10027888500

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos.2010

    • Author(s)
      Satoshi Sugimura, Ken-ichi Yamanaka, Manabu Kawahara, Takuya Wakai, Masaki Yokoo, Eimei Sato.
    • Journal Title

      Animal Science Journal 81

      Pages: 48-57

    • NAID

      10027888500

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Longevity in mice without a father2010

    • Author(s)
      Manabu Kawahara, Tomohiro Kono.
    • Journal Title

      Human Reproduction 25

      Pages: 457-461

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of insulin-like growth Factor 2 in angiogenic factor transcription in bi-maternal mouse conceptuses.2010

    • Author(s)
      Manabu Kawahara, Qiong Wu, Tomohiro Kono.
    • Journal Title

      Journal of Reproduction and Development 56

      Pages: 79-85

    • NAID

      10026323565

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anomalous oxygen consumption in porcine somatic cell nuclear transferembryos.2010

    • Author(s)
      Satoshi Sugimura, Masaki Yokoo, Ken-ichi Yamanaka, Manabu Kawahara, Satoru Moriyasu, Takuya Wakai, Takashi Nagai, Hiroyuki Abe, Eimei Sato.
    • Journal Title

      Cellular Reprogramming (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos2009

    • Author(s)
      山中謙一, 杉村智志, 若井拓哉, 川原学, 佐藤英明
    • Journal Title

      Journal of Reproduction and Development 55巻

      Pages: 638-644

    • NAID

      10026322712

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Defining contributions of paternally methylated imprinted genes at the Igf2-H19 and Dlk1-Gtl2 domains to mouse placentation by transcriptomic analysis2009

    • Author(s)
      川原学, 森田慎之介, 高橋望, 河野友宏
    • Journal Title

      Journal of Biological Chemistry 284巻

      Pages: 17751-17765

    • NAID

      120006377532

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos.2009

    • Author(s)
      Ken-ichi Yamanaka, Satoshi Sugimura, Takuya Wakai, Manabu Kawahara, Eimei Sato.
    • Journal Title

      Journal of Reproduction and Development 55

      Pages: 638-644

    • NAID

      10026322712

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defining contributions of paternally methylated imprinted genes at the IGF2-H19 and DLK1-GT12 domains to mouse placentation by transcriptomic analysis2009

    • Author(s)
      Manabu Kawahara, Shinnosuke Morita, Nozomi Takahashi, Tomohiro Kono
    • Journal Title

      The Journal of Biological Chemistry (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 二母性マウスの長命性について2009

    • Author(s)
      河野友宏, 塚平俊貴, 川原学
    • Organizer
      第102回日本繁殖生物学会
    • Place of Presentation
      奈良
    • Year and Date
      2009-09-12
    • Related Report
      2009 Final Research Report
  • [Presentation] 二母性マウスの長命性について2009

    • Author(s)
      河野友宏, 塚平俊貴, 川原学
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      奈良
    • Year and Date
      2009-09-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 二母性マウス胚由来胎盤における網羅的遺伝子発現解析2009

    • Author(s)
      川原学, 森田慎之助, 高橋望, 河野友宏
    • Organizer
      第102回日本繁殖生物学会
    • Place of Presentation
      奈良
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス二母性胚の発生に対する父性メチル化制御インプリント遺伝子の発現アレルの影響2009

    • Author(s)
      塚平俊貴, 川原学, 河野友宏
    • Organizer
      第50回日本哺乳動物卵子学会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 二母性マウス胚由来胎盤における網羅的遺伝子発現解析2009

    • Author(s)
      川原学, 森田慎之助, 高橋望, 河野友宏
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      奈良
    • Related Report
      2009 Annual Research Report
  • [Presentation] 精子無し"マウスの作り方. 日本動物学会九州支部2008

    • Author(s)
      川原学
    • Organizer
      日本植物学会九州支部および日本生態学会九州地区三部合同例会
    • Place of Presentation
      佐賀
    • Year and Date
      2008-11-08
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19881219?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=3

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19700928?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=4

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/20163672?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=1

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19952375?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=2

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19380578?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPane1.Pubmed_DefaultReportPanel.Pubmed-RVDocSum

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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