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Isolation and characterized of the porcine NKT cells

Research Project

Project/Area Number 20780213
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

OGAWA Tomoko  National Institute of Agrobiological Sciences, 家畜ゲノム研究ユニット, 任期付研究員 (90466011)

Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥5,675,659 (Direct Cost: ¥4,365,892、Indirect Cost: ¥1,309,767)
Fiscal Year 2010: ¥1,385,659 (Direct Cost: ¥1,065,892、Indirect Cost: ¥319,767)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords免疫学 / 獣医学 / ブタ / NKT細胞 / CD1
Research Abstract

In human and mouse, NKT cells are characterized as a population that can be stimulated by glycolipid antigen, such as α-Galactosylceramide (α-GalCer), which is presented by CD1d molecule. Activated NKT cells can produce both cytokines (IFN-γ and IL-4) and cytolytic protein (perforin), suggesting that this cell population have an important role in both innate and acquired immunity. In this study, we tried to conduct isolation, culture and functional analysis of porcine NKT cells. Flow cytometry analysis showed that there are T cell marker (CD3^+)-expressing cells in porcine NK cell (CD8^+CD16^+) population. In addition, we detected mouse CD1d tetramer-reactive cells in porcine CD3^+ cell population. Furthermore, we obtained the homologous sequence of human NKT cell markers, TCRVα24 and TCRVβ11 from pig PBMC. The amino acid sequences that are important for NKT cell binding to α-GalCer and CD1d were conserved in the obtained porcine TcR sequences. These results strongly suggested the existence of porcine NKT cells.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (5 results)

All 2010 2009 2008 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Genomic structure of the whole D-J-C clusters and the upstream region coding V segments of the TRB locus in pig.2009

    • Author(s)
      Eguchi-Ogawa T, Toki D, Uenishi H
    • Journal Title

      Developmental & Comparative Immunology 33

      Pages: 1111-1119

    • Related Report
      2010 Final Research Report
  • [Journal Article] Genomic structure of the whole D-J-C clusters and the upstream region Coding V segments of the TRB locus in pig.

    • Author(s)
      T.Eguchi-Ogawa, et al.
    • Journal Title

      Developmental & Comparative Immunology (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] V_H、D_HおよびJ_H領域を含むブタ抗体重鎖のゲノム構造2010

    • Author(s)
      小川智子
    • Organizer
      第9回 国際獣医免疫学会議
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2010-08-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] There are three TRBD-TRBJ-TRBC clusters in porcine TRB gene2008

    • Author(s)
      T. Eguchi-Ogawa, et al.
    • Organizer
      ISAG2008(第31回 国際動物遺伝学会議)
    • Place of Presentation
      オランダ アムステルダム市
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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