| Project/Area Number |
20780221
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | Azabu University |
|---|
Principal Investigator |
SAKAUE Motohara Azabu University, 獣医学部, 准教授 (60348589)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 脳由来神経影響因子 / 神経細胞死 / 小脳顆粒細胞 / TrkB / 初代培養系 / 神経芽細胞腫由来B35細胞 / ラット / 脳由来神経栄養因子 / 小脳粒細胞 / 神経細胞腫由来B35細胞 |
|---|
| Research Abstract |
Brain-derived neurotrophic factor (BDNF) is a maintenance protein for neuronal function and survive, but also stimulate methylmercury-induced neuronal cell death. In the present study, to expose mechanisms of the BDNF stimulation, we investigate using rat cerebellar granular cell culture system. Neurotrophin-4, another TrkB ligand, stimulated methylmercury-induced cell death. The stimulating effect of BDNF on the cell death was inhibited by treatment of anti-BDNF antibody that prevents BDNF from binding to TrkB. The results indicated that the stimulation by BDNF needs the BDNF binding to TrkB, which suggested BDNF/TrkB play as a sensitivity determiant factor.
|
