Role of keratinocytes in the development of atopic dermatitis

• Project/Area Number: 20780224
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: OHMORI Keitaro Tokyo University of Agriculture and Technology, 大学院・共生科学技術研究院, 助教 (20466915)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アレルギー / 免疫 / 炎症 / 皮膚 / 動物

Research Abstract

The importance of keratinocytes in the development of atopic dermatitis was evaluated by using a murine model of atopic dermatitis and dogs. We found that increased cell proliferation of keratinocytes and the local crosstalk between keratinocytes and mast cells in the skin were pivotal in the pathogenesis and clinical signs of atopic dermatitis. We also revealed that improvement of skin barrier function could be effective for alleviating atopic dermatitis and would be a local therapeutic option.

Research Products

• [Journal Article] Pilot evaluation of the efficacy of shampoo treatment with ultra-pure soft water for canine pruritus (2010)
  • Author(s): Ohmori K, Tanaka A, Makita Y, Takai M, Yoshinari Y, Matsuda H.
  • Journal Title: Veterinary Dermatology (in press)
  • Peer Reviewed
• [Journal Article] A novel NF-κB inhibitor improves glucocorticoid 1 sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors (2010)
  • Author(s): Matsuda A, Tanaka A, Muto S, Ohmori K, Furusaka T, Jung K, Karasawa K, Okamoto N, Oida K, Itai A, Matsuda H.
  • Journal Title: Research in Veterinary Science (in press)
  • Peer Reviewed
• [Journal Article] Cultivation and characterization of canine skin-derived mast cells (2010)
  • Author(s): Kawarai S, Masuda K, Ohmori K, Matsuura S, Yasuda N, Nagata M, Sakaguchi M, Tsujimoto H.
  • Journal Title: Journal of Veterinary Medical Science 72(2 Pages: 131-140
  • NAID: 130000134517
  • Peer Reviewed
• [Journal Article] Pilot evaluation of the efficacy of shampoo treatment with ultra-pure soft water for canine pruritus (2010)
  • Author(s): Ohmori K, Tanaka A, Makita Y, Takai M, Yoshinari Y, Matsuda H.
  • Journal Title: Veterinary Dermatology (In press)
  • Peer Reviewed
• [Journal Article] A novel NF-κB inhibitor improves glucocorticoid 1 sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors (2010)
  • Author(s): Matsuda A, Tanaka A, Muto S, Ohmori K, Furusaka T, Jung K, Karasawa K, Okamoto N, Oida K, Itai A, Matsuda H.
  • Journal Title: Research in Veterinary Science (In press)
  • Peer Reviewed
• [Journal Article] Cultivation and characterization of canine skin-derived mast cells (2010)
  • Author(s): Kawarai S, Masuda K, Ohmori K, Matsuura S, Yasuda N, Nagata M, Sakaguchi M, Tsujimoto H.
  • Journal Title: Journal of Veterinary Medical Science 72(2) Pages: 131-140
  • NAID: 130000134517
  • Peer Reviewed
• [Journal Article] IL-3 induces basophil expansion in vivo by directing granulocyte-monocyte progenitors to differentiate into basophil lineage-restricted progenitors in the bone marrow and by increasing the number of basophil/mast cell progenitors in the spleen (2009)
  • Author(s): Ohmori K, Luo Y, Jia Y, Nishida J, Wang Z, Bunting KD, Wang D, Huang H.
  • Journal Title: The Journal of Immunology 182(5) Pages: 2835-41
  • Peer Reviewed
• [Journal Article] Oral supplementation with Lactobacillus rhamnosus CGMCC 1. 3724 prevents development of atopic dermatitis in NC/NgaTnd mice possibly by modulating local production of IFN-γ (2009)
  • Author(s): Tanaka A, Jung K, Benyacoub J, Prioult G, Okamoto N, Ohmori K, Blum S, Mercenier A, Matsuda H.
  • Journal Title: Experimental Dermatology 18(12) Pages: 1022-7
  • Peer Reviewed
• [Journal Article] IL-3 induces basophil expansion in vivo by directing granulocyte-monocyte progenitors to differentiate into basophil lineage-restricted progenitors in the bone marrow and by increasing the number of basophil/mast cell progenitors in the spleen (2009)
  • Author(s): Ohmori K, Luo Y, Jia Y, Nishida J, Wang Z, Bunting KD, Wang D, Huang H
  • Journal Title: The Journal of Immunology 182 Pages: 2835-41
  • Peer Reviewed
• [Journal Article] Oral supplementation with Lactobacillus rhamnosus CGMCC 1.3724 prevents development of atopic dermatitis in NC/NgaTnd mice possibly by modulating local production of IFN-γ (2009)
  • Author(s): Tanaka A, Jung K, Benyacoub J, Prioult G, Okamoto N, Ohmori K, Blum S, Mercenier A, Matsuda H
  • Journal Title: Experimental Dermatology (In press)
  • Peer Reviewed
• [Journal Article] Identification of c-kit mutations-independent neoplastic cell proliferation of canine mast cells (2008)
  • Author(s): Ohmori K, Kawarai S, Yasuda N, Tanaka A, Matsuda H, Nishimura R, Sasaki N, Tsujimoto H, Masuda K.
  • Journal Title: Veterinary Immunology and Immunopathology 126(1-2) Pages: 43-53
  • Peer Reviewed
• [Journal Article] Identification of c-kit mutations-independent neoplastic cell proliferation of canine mast cells (2008)
  • Author(s): Ohmori K, Kawarai S, Yasuda N, Tanaka A, Matsuda H, Nishimura R, Sasaki N, Tsuiimoto H, Masuda K
  • Journal Title: Veterinary Immunology and Immunopathology 126 Pages: 43-53
  • Peer Reviewed
• [Presentation] 皮膚炎自然発症犬を用いた高純度軟化水による皮膚炎改善効果の検証 (2009)
  • Author(s): 大森啓太郎、田中あかね、巻田優花、高井政貴、吉成佑治、松田浩珍
  • Organizer: 第59回日本アレルギー学会秋季学術大会
  • Place of Presentation: 秋田キャッスルホテル(秋田県)
• [Presentation] イヌの痒みに対する高純度軟化水を用いたシャンプー療法の臨床的効果 (2009)
  • Author(s): 大森啓太郎、田中あかね、巻田優花、高井政貴、吉成佑治、松田浩珍
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: とりぎん文化会館(鳥取県)
• [Presentation] 高純度軟化水(UPSW)のアトピー性皮膚炎改善効果4. 皮膚バリア損傷モデルでの検証 (2008)
  • Author(s): 田中あかね、大森啓太郎、高井政貴、吉成佑治、松田浩珍
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京都千代田区東京国際フォーラム
  • Year and Date: 2008-11-28
• [Presentation] ヒトおよびイヌの皮膚バリア機能に及ぼす高純度軟化水の影響 (2008)
  • Author(s): 大森啓太郎、田中あかね、高井正貴、吉成佑治、松田浩珍
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎県宮崎市ワールドコンベンションセンター
  • Year and Date: 2008-09-25
• [Presentation] 高純度軟化水(UPSW)のアトピー性皮膚炎改善効果4. 皮膚バリア損傷モデルでの検証 (2008)
  • Author(s): 田中あかね、大森啓太郎、高井政貴、吉成佑治、松田浩珍
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京国際フォーラム(東京都)
• [Presentation] ヒトおよびイヌの皮膚バリア機能に及ぼす高純度軟化水の影響 (2008)
  • Author(s): 大森啓太郎、田中あかね、高井正貴、吉成佑治、松田浩珍
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: ワールドコンベンションセンター(宮崎県)