Technological development for promoting phosphate recovery from wastewater by using symbiosis of Sesbania rostrata and Azorhizobium caulinodans

• Project/Area Number: 20780231
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Boundary agriculture
• Research Institution: The University of Tokyo
• Principal Investigator: AONO Toshihiro The University of Tokyo, 生物生産工学研究センター, 助教 (10372418)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 資源循環システム / 環境技術 / 廃棄物再資源化 / 応用微生物 / 共生細菌 / 病原菌

Research Abstract

Azorhizobium caulinodans is a microsymbiont of a tropical legume, Sesbania rostrata, and forms nitrogen fixing nodules on the stems. In this study, it was revealed that praR gene of A. caulinodans was involved in suppression of reb genes, and that an A. caulinodans praR mutant exhibiting high expression of reb genes changed from a symbiont to a pathogen. It was also found that an A. caulinodans mutant taking up potassium in ATP-dependent manner formed stem-nodules showing nitrogen fixation.

Research Products

• [Journal Article] phrR-like gene praR of Azorhizobium caulinodans ORS571 is essential for symbiosis with Sesbania rostrata and is involved in expression of reb genes. (2010)
  • Author(s): Akiba N, Aono T, Toyazaki H, Sato S, Oyaizu H
  • Journal Title: Applied and Environmental Microbiology 76 Pages: 3475-3485
  • Peer Reviewed
• [Journal Article] phrR-like gene praR of Azorhizobium caulinodans ORS571 isessential for symbiosis with Sesbania rostrata and is involved in expression of reb genes. (2010)
  • Author(s): Akiba N, Aono T, Toyazaki H, Sato S, Oyaizu H
  • Journal Title: Applied and Environmental Microbiology Volume: 76 Pages: 3475-3485
  • Peer Reviewed
• [Journal Article] Comparative genome-wide transcriptional profiling of Azorhizobium caulinodans ORS571 grown under free-living and symbiotic conditions. (2009)
  • Author(s): Tsukada S, Aono T, Akiba N, Lee KB, Liu CT, Toyazaki H, Oyaizu H
  • Journal Title: Applied and Environmental Microbiology 75 Pages: 5037-5046
  • Peer Reviewed
• [Journal Article] Comparative genome-wide transcriptional profiling of Azorhizobium caulinodans ORS571 grown under free-living and symbiotic conditions (2009)
  • Author(s): Tsukada S, Aono T, Akiba N, Lee KB, Liu CT, Toyazaki H, Oyaizu H
  • Journal Title: Applied and Environmental Microbiology Volume: 75 Pages: 5037-5046
  • Peer Reviewed
• [Journal Article] An outer membrane autotransporter, AoaA, of Azorhizobium caulinodans is required for sustaining high N_2-fixing activity of stem nodules. (2008)
  • Author(s): Suzuki T, Aono T, Liu CT, Suzuki S, Iki T, Yokota K, Oyaizu H
  • Journal Title: FEMS Microbiology Letters 285 Pages: 16-24
  • Peer Reviewed
• [Journal Article] An outer membrane autotransporter, AoaA, of Azorhizobium caulinodans is required for sustaining high N2-fixing activity of stem nodules. (2008)
  • Author(s): Suzuki T, Aono T, Liu CT, Suzuki S. Iki T. Yokota K. Ovaizu H
  • Journal Title: FEMS Microbiology Letters 285 Pages: 16-24
  • Peer Reviewed
• [Presentation] The praR gene of Azorhizobium caulinodans ORS571 is essential for symbiosis with Sesbania rostrata, and is involved in expression of reb genes. (2010)
  • Author(s): Noriko Akiba, Toshihiro Aono, Hiroki Toyazaki, Satoru Sato, Hiroshi Oyaizu
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and NitrogenFixation
  • Place of Presentation: 青島パームビーチホテル(宮崎)
  • Year and Date: 2010-09-21
• [Presentation] The praR gene of Azorhizobium caulinodans ORS571 is essential for symbiosis with Sesbania rostrata, and is involved in expression of reb genes. (2010)
  • Author(s): Noriko Akiba, Toshihiro Aono, Hiroki Toyazaki, Satoru Sato, Hiroshi Oyaizu
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 宮崎
  • Year and Date: 2010-09-20
• [Presentation] Azorhizobium caulinodans ORS571におけるphrR遺伝子の機能解明(2) (2009)
  • Author(s): 秋葉紀子、青野俊裕、小柳津広志
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-15
• [Presentation] Azorhlzobium caulinodans ORS571におけるphrR遺伝子の機能解明(2) (2009)
  • Author(s): 秋葉紀子、青野俊裕、小柳津広志
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学(京都)
  • Year and Date: 2009-09-15
• [Presentation] Azorhizobium caulinodans ORS571におけるphrR遺伝子の機能解析 (2008)
  • Author(s): 秋葉紀子、青野俊裕、小柳津広志
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-09
• [Presentation] Azorhizobium caulinodans ORS571におけるカリウム吸収関連遺伝子群の機能解析 (2008)
  • Author(s): 戸矢崎裕希、青野俊裕、小柳津広志
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-09
• [Presentation] Azorhizobium caulinodans ORS571におけるマイクロアレイを用いた遺伝子発現解析 (2008)
  • Author(s): 塚田周平、青野俊裕、小柳津広志
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-09