Epigenetics of plant mixoploidy

• Project/Area Number: 20780240
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Osaka Kyoiku University
• Principal Investigator: SUZUKI Go 大阪教育大学, 教育学部, 准教授 (10314444)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 遺伝子 / 染色体工学 / 育種学 / 遺伝学 / 植物 / 染色体 / バイオテクノロジー / エピジェネティクス / 形質転換体 / タバコ

Research Abstract

We showed that mixoploidy is inherited in the transgenic tobacco, and analyzed epigenetic status at a chromosomal level. We also performed FISH and sequencing analyses of mixoploid plants with tobacco BAC clones. From these analyses, we discussed the mechanisms of producing mixoploidy in the tobacco, which might be related to the epigenetic status at chromosomal and DNA levels.

Research Products

• [Journal Article] Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species (2012)
  • Author(s): Suzuki, G., Ogaki, Y., Hokimoto, N., Xiao, L., Kikuchi-Taura, A., Harada, C., Okayama, R., Tsuru, A., Onishi, M., Saito, N., Do, G. S., Lee, S. H., Ito, T., Kanno, A., Yamamoto, M., and Mukai, Y.
  • Journal Title: Plant Cell Rep Volume: 31 Pages: 621-628
  • Peer Reviewed
• [Journal Article] Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species (2012)
  • Author(s): Suzuki, G., et al
  • Journal Title: Plant Cell Report Volume: 31 Issue: 4 Pages: 621-628
  • DOI: 10.1007/s00299-011-1178-8
  • Peer Reviewed
• [Journal Article] Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres (2011)
  • Author(s): Nagaki, K., Shibata, F., Suzuki, G., Kanatani, A., Ozaki, S., Hironaka, A., Kashihara, K., and Murata, M.
  • Journal Title: Chromosome Res Volume: 19 Pages: 591-605
  • Peer Reviewed
• [Journal Article] Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii (2011)
  • Author(s): Suzuki, G., Wada, H., Goto, H., Nakano, A., Oba, H., Deno, T., Rahman, S., and Mukai, Y.
  • Journal Title: Plant Cell Rep Volume: 30 Pages: 2293-2301
  • Peer Reviewed
• [Journal Article] Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres (2011)
  • Author(s): Nagaki, K., et al
  • Journal Title: Chromosome Research Volume: 19 Issue: 5 Pages: 591-605
  • DOI: 10.1007/s10577-011-9219-2
  • Peer Reviewed
• [Journal Article] Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii (2011)
  • Author(s): Suzuki, G., et al
  • Journal Title: Plant Cell Report Volume: 30 Issue: 12 Pages: 2293-2301
  • DOI: 10.1007/s00299-011-1134-7
  • Peer Reviewed
• [Journal Article] Characterization of CDC48 in Allium cepa (2010)
  • Author(s): Suzuki, G., Shiomi, M., Morihana, S., and Mukai, Y.
  • Journal Title: Chromosome Sci Volume: 13 Pages: 27-29
  • NAID: 10028105747
  • Peer Reviewed
• [Journal Article] DNA methylation and histone modification in onion chromosomes (2010)
  • Author(s): Suzuki, G., Shiomi, M., Morihana, S., Yamamoto, M., and Mukai, Y.
  • Journal Title: Genes Genet. Syst Volume: 85 Pages: 377-382
  • NAID: 10029515991
  • Peer Reviewed
• [Journal Article] DNA methylation and histone modification in onion chromosomes. (2010)
  • Author(s): Suzuki, G., Shiomi, M., Morihana, S., Yamamoto, M., Mukai, Y.
  • Journal Title: Genes Genet.Syst. 85 Pages: 377-382
  • NAID: 10029515991
  • Peer Reviewed
• [Journal Article] DNA methylation and histone modification in onion chromosomes (2010)
  • Author(s): Suzuki, G., et al.
  • Journal Title: Genes & Genetic Systems Volume: 85 Pages: 377-382
  • NAID: 10029515991
  • Peer Reviewed
• [Journal Article] Visualization of multiple T-DNA loci by FISH on extended DNA fibers (2009)
  • Author(s): Imazawa, T., Suzuki, G., Nakano, A., Yamamoto, M., and Mukai, Y.
  • Journal Title: Plant Biotech Volume: 26 Pages: 421-425
  • NAID: 10026136261
  • Peer Reviewed
• [Journal Article] Recent progress in plant reproduction research : the story of the male gametophyte through to successful fertilization (2009)
  • Author(s): Suzuki, G.
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 1857-1864
  • NAID: 10027343986
  • Peer Reviewed
• [Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes (2009)
  • Author(s): Wada, N., Kajiyama, S., Akiyama, Y., Kawakami, S., No, D., Uchiyama, S., Otani, M., Shimada, T., Nose, N., Suzuki, G., Mukai, Y., and Fukui, K.
  • Journal Title: Plant Cell Rep Volume: 28 Pages: 759-768
  • Peer Reviewed
• [Journal Article] Visualization of multiple T-DNA loci by FISH on extended DNA fibers. (2009)
  • Author(s): Imazawa, T., Suzuki, G., et al.
  • Journal Title: Plant Biotechnology 26 Pages: 421-425
  • NAID: 10026136261
  • Peer Reviewed
• [Journal Article] Recent progress in plant reproduction research : the story of the male gametophyte through to successful fertilization. (2009)
  • Author(s): Suzuki, G.
  • Journal Title: Plant and Cell Physiology 50 Pages: 1857-1864
  • NAID: 10027343986
  • Peer Reviewed
• [Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes. (2009)
  • Author(s): Wada, N., Kajiyama, S., Akiyama, Y., Kawakami, S., No, D., Uchiyama, S., Otani, M., Shimada, T., Nose, N., Suzuki, G., et al.
  • Journal Title: Plant Cell Report 28 Pages: 759-768
  • Peer Reviewed
• [Journal Article] 大きな植物染色体上でDNAを光らせる挑戦 (2008)
  • Author(s): 鈴木剛
  • Journal Title: 生物の科学・遺伝 Volume: 62 Pages: 73-77
• [Journal Article] Pilot sequencing of onion genomic DNA reveals fragments of transposable elements, low gene densities, and significant gene enrichment after methyl filtration (2008)
  • Author(s): Jakse, J., Meyer, J. D. F., Suzuki, G., McCallum, J., Cheung, F., Town, C. D. and Havey, M. J.
  • Journal Title: Mol. Genet. Genomics Volume: 280 Pages: 287-292
  • Peer Reviewed
• [Journal Article] Separated transcriptomes of male gametophyte and tapetum in rice : validity of a laser microdissection(LM) microarray (2008)
  • Author(s): Suwabe, K., Suzuki, G., Takahashi, H., Shiono, K., Endo, M., Yano, K., Fujita, M., Masuko, H., Saito, H., Fujioka, T., Kaneko, F., Kazama, T., Mizuta, Y., Kawagishi-Kobayashi, M., Tsutsumi, N., Kurata, N., Nakazono, M. and Watanabe, M.
  • Journal Title: Plant Cell Physiol Volume: 49 Pages: 1407-1416
  • NAID: 10027333829
  • Peer Reviewed
• [Journal Article] Mixoploidy in transgenic tobacco. (2008)
  • Author(s): Suzuki, G., Tsujii, Y., Miwa, M., Saito, Y., Imazawa, T., Nakano, A., Yamamoto M., Mukai, Y.
  • Journal Title: Adv.Chromosome Sci. 3 Pages: 133-133
• [Journal Article] 大きな植物染色体上でDNAを光らせる挑戦 (2008)
  • Author(s): 鈴木剛
  • Journal Title: 生物の科学・遺伝 62 Pages: 73-77
• [Journal Article] Pilot sequencing of onion genomic DNA reveals fragments of transposable elements, low gene densities, and significant gene enrichment after methyl filtration (2008)
  • Author(s): Jakse, J., Meyer, J. D. F., Suzuki, G., et.al.
  • Journal Title: Mol. Genet. Genomics 280 Pages: 287-292
  • Peer Reviewed
• [Journal Article] Separated transcriptomes of male gametophyte and tapetum in rice : validity of a laser microdissection (LM) microarray (2008)
  • Author(s): Suwabe, K., Suzuki, G. et.al.
  • Journal Title: Plant Cell Physiol 49 Pages: 1407-1416
  • NAID: 10027333829
  • Peer Reviewed
• [Presentation] タバコ動原体由来BACの塩基配列解析によるタバコ動原体特異的反復配列の同定 (2010)
  • Author(s): 長岐清孝, 柴田洋, 鈴木剛, 村田稔
  • Organizer: 第61回染色体学会年会
  • Place of Presentation: 船橋
  • Year and Date: 2010-11-07
• [Presentation] 形質転換タバコに見られる混数性 (2009)
  • Author(s): 佐々井直人, 斉藤優, 三輪美佳, 辻井由香, 鈴木剛
  • Organizer: 第60回染色体学会年会
  • Place of Presentation: 松江
  • Year and Date: 2009-11-12
• [Presentation] Mixoploidy in transgenic tobacco (2008)
  • Author(s): Suzuki, G., Tsujii, Y., Miwa, M., Saito, Y., Imazawa, T., Nakano, A., Yamamoto M. and Mukai, Y.
  • Organizer: 3rd Asian Chromosome Colloquium
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2008-12-01
• [Presentation] Mixoploidy in transgenic tobacco. (2008)
  • Author(s): Suzuki, G.
  • Organizer: 3rd Asian Chromosome Colloquium
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2008-12-01
• [Presentation] Mixoploidy in transgenic tobacco (2008)
  • Author(s): Suzuki, G.
  • Organizer: 3^<rd> Asian Chromosome Colloquium
  • Place of Presentation: 大阪
  • Year and Date: 2008-12-01