Identification of stress tolerance related genes via FOX hunting of Thellungiella halophila full-length cDNA
Project/Area Number |
20780242
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied molecular and cellular biology
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
TAJI Teruaki Tokyo University of Agriculture, 応用生物科学部, 助教 (60360583)
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Project Period (FY) |
2008 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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Keywords | 遺伝子資源 / 環境ストレス耐性 / 耐塩性 / トランスジェニック植物 / 塩生植物 / 植物 / 環境 / ストレス / バイオテクノロジー / 発現制御 |
Research Abstract |
Thellungiella halophila has been identified as a model system for understanding abiotic stress tolerance, and it shows extreme salt tolerance. Recently, full-length cDNA library with a total of 9,569 unique genes were constructed from Thellungiella plants treated with salinity, cold, freezing stresses or ABA treatment (Taji et al.,2008). Using ectopic expression of full-length cDNAs, a novel gain-of-function system, termed the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system) was developed. We identified 3 salt-, 2 osmo- and 4 heat-tolerance related genes using the FOX hunting.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Dissecting the genetic control of natural variation in salt tolerance of Arabidopsis thaliana accessions.2010
Author(s)
Katori, T., Ikeda, A., Iuchi, S., Kobayashi, M., Shinozaki, K., Maehashi, K., Sakata, Y., Tanaka, S., Taji, T
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Journal Title
J Exp Bot 61
Pages: 1125-1138
Related Report
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[Journal Article] Functional analyses of the ABI1-related protein phosphatase type 2C reveal evolutionarily conserved regulation of abscisic acid signaling between Arabidopsis and the moss Physcomitrella patens.2009
Author(s)
Komatsu, K., Nishikawa, Y., Ohtsuka, T., Taji, T., Quatrano, R.S., Tanaka, S., Sakata, Y.
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Journal Title
Plant Mol Biol 70
Pages: 327-340
Related Report
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[Journal Article] Large-scale collection and annotation of full-length enriched cDNAs from a model halophyte, Thellungiella halophila.2008
Author(s)
Taji, T., Sakurai, T., Totoki, Y., Toyoda, A., Sakaki, Y., Seki, M., Sakata, Y., Tanaka, S., Shinozaki, K.
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Journal Title
BMC Plant Biol. 12
Pages: 1-15
Related Report
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[Journal Article] Large-scale collection and annotation of full-length enriched cDNAs from a model halophyte, Thellungiella halophila2008
Author(s)
Taji T, Sakurai T, Mochida K, Ishiwata A, Kurotani A, Totoki Y, Toyoda A, Sakaki Y, Seki M, Ono H, Sakata Y, Tanaka S, Shinozaki K.
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Journal Title
BMC Plant Biol 12 ; 8:115
Pages: 1-12
Related Report
Peer Reviewed
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