Project/Area Number |
20780242
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Applied molecular and cellular biology
|
Research Institution | Tokyo University of Agriculture |
Principal Investigator |
TAJI Teruaki Tokyo University of Agriculture, 応用生物科学部, 助教 (60360583)
|
Project Period (FY) |
2008 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
Keywords | 遺伝子資源 / 環境ストレス耐性 / 耐塩性 / トランスジェニック植物 / 塩生植物 / 植物 / 環境 / ストレス / バイオテクノロジー / 発現制御 |
Research Abstract |
Thellungiella halophila has been identified as a model system for understanding abiotic stress tolerance, and it shows extreme salt tolerance. Recently, full-length cDNA library with a total of 9,569 unique genes were constructed from Thellungiella plants treated with salinity, cold, freezing stresses or ABA treatment (Taji et al.,2008). Using ectopic expression of full-length cDNAs, a novel gain-of-function system, termed the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system) was developed. We identified 3 salt-, 2 osmo- and 4 heat-tolerance related genes using the FOX hunting.
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