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Development of a Sequence-specific detection probe of damaged nucleobase for noninvasive DNA damage imaging.

Research Project

Project/Area Number 20790010
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Chemical pharmacy
Research InstitutionOsaka University

Principal Investigator

KODAMA Tetsuya  Osaka University, 薬学研究科, 助教 (00432443)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords生体関連物質 / 人工核酸 / 分子認識 / 酸化損傷塩基 / 可視化 / 三重鎖 / 機能性核酸 / 8-OxoG
Research Abstract

To develop a sequence-specific detection method of damaged nucleobase for noninvasive DNA damage imaging, we successfully created a novel bridged nucleic acid monomer that can recognize 8-hydroxyguanine in double-stranded DNA in a sequence-specific manner. Additionally, several nucleic acids, which convert into an oxidative lesion 5-formyluridne in double-stranded DNA in response to photo irradiation, were also synthesized. Moreover, we designed and synthesized a triplex forming oligonucleotide probe by organizing the 8-hydroxyguanine-recognizing nucleic acid monomer and acid-labile phosphoramidite linkage, and the fluorescent labeling of the probe was achieved for its noninvasive imaging use.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (19 results)

All 2010 2009 2008

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (11 results)

  • [Journal Article] Synthesis of Light-responsive Bridged Nucleic Acid and Changes in Affinity with Complementary ssRNA.2009

    • Author(s)
      Kunihiko Morihiro, Tetsuya Kodama, Masaru Nishida, Takeshi Imanishi, Satoshi Obika
    • Journal Title

      ChemBioChem 10

      Pages: 1784-1788

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Design, Synthesis, and Evaluation of a Novel Bridged Nucleic Acid, 2', 5'-BNAON, with S-type Sugar Conformation Fixed by N-O Linkage.2009

    • Author(s)
      Tetsuya Kodama, Chieko Matsuo, Hidetsugu Ori, Tetsuya Miyoshi, Satoshi Obika, Kazuyuki Miyashita, Takeshi Imanishi
    • Journal Title

      Tetrahedron 65

      Pages: 2116-2123

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of a Novel trans-3', 4'-BNA Monomer Bearing a 4, 8-Dioxa-5-azabicyclo [5. 3. 0] decane Skeleton.2009

    • Author(s)
      Tetsuya Kodama, Kensaku Sugaya, Takeshi Baba, Takeshi Imanishi, Satoshi Obika
    • Journal Title

      Heterocycles 79

      Pages: 873-882

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Light-Responsive Bridged Nucleic Acid and Changes in Affinity with Complementary ssRNA2009

    • Author(s)
      Kunihiko Morihiro(研究補助)
    • Journal Title

      ChemBioChem 10

      Pages: 1784-1788

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design, synthesis, and evaluation of a novel bridged nucleic acid, 2',5'- BNA^<0N>, with S-type sugar conformation fixed by N-O linkage2009

    • Author(s)
      Tetsuya Kodama
    • Journal Title

      Tetrahedron 65

      Pages: 2116-2123

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of a novel trans-3', 4'-BNA monomer bearing a 4,8-dioxa-5-azabicyclo[5.3.0]decane skeleton2009

    • Author(s)
      Tetsuya Kodama
    • Journal Title

      Heterocycles 79(印刷中)

    • NAID

      40016543114

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and Chemical Synthesis of a 2', 4'-BNA Probe for 7, 8-Dihydro-8-oxoguanine-containing Double Stranded DNA.2008

    • Author(s)
      Tetsuya Miyoshi, Tetsuya Kodama, Satoshi Obika, Takeshi Imanishi
    • Journal Title

      Nucleic Acids Symp. Ser. 52

      Pages: 305-306

    • Related Report
      2009 Final Research Report
  • [Journal Article] Design and chemical synthesis of a 2', 4'-BNA probe for 7,8-dihydro-8-oxoguanine-containing double stranded DNA2008

    • Author(s)
      Tetsuya Miyoshi (Tetsuya Kodama)
    • Journal Title

      Nucleic Acids Symp. Ser 52

      Pages: 305-306

    • Related Report
      2008 Annual Research Report
  • [Presentation] 高感度生体情報分析技術としてのSNP分析及び損傷塩基イメージング法の開発2010

    • Author(s)
      伊藤浩介(研究補助)
    • Organizer
      第3回創薬とイメージングに関するシンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 次世代核酸系機能素材の創製を目指した高度機能化人工核酸の設計と合成2009

    • Author(s)
      兒玉哲也
    • Organizer
      第33回大学分析者の会
    • Place of Presentation
      大阪
    • Year and Date
      2009-07-29
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 三重鎖形成を基盤とした迅速かつ簡便なSNP分析法2009

    • Author(s)
      伊藤浩介、戸水真治、根来佳憲、折田文子、兒玉哲也、今西武、小比賀聡
    • Organizer
      遺伝子・デリバリー研究会第9回シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2009-07-11
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 3-オキサ-4-アザペンチレン架橋構造を有する新しいtrans-3', 4'-BNAの設計と合成2009

    • Author(s)
      兒玉哲也、菅谷建作、馬場武、今西武、小比賀聡
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] 8-OxoGの配列特異的認識能を有する三重鎖形成人工核酸の開発(1)2008

    • Author(s)
      三好哲也, 兒玉哲也, 小比賀聡, 今西武
    • Organizer
      第58回日本薬学会近畿支部大会
    • Place of Presentation
      兵庫
    • Year and Date
      2008-10-25
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 8-OxoGの配列特異的認識能を有する三重鎖形成人工核酸の開発(2)2008

    • Author(s)
      兒玉哲也, 三好哲也, 小比賀聡, 今西武
    • Organizer
      第58回日本薬学会近畿支部大会
    • Place of Presentation
      兵庫
    • Year and Date
      2008-10-25
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 二重鎖DNA中の8-オキソグアニン塩基を選択的に認識する人工核酸プローブ2008

    • Author(s)
      兒玉哲也
    • Organizer
      第11回生命化学研究会
    • Place of Presentation
      群馬
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Design and Chemical Synthesis of a 2', 4'-BNA Probe for 7, 8-Dihydro-8-oxoguanine-containing Double Stranded DNA, Joint symposium of 18th International Roundtable on Nucleosides2008

    • Author(s)
      Tetsuya Miyoshi, Tetsuya Kodama, Satoshi Obika, Takeshi Imanishi
    • Organizer
      Nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chelistry
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] DNA配列選択的な8-ヒドロキシグアニン検出プローブの創製研究2008

    • Author(s)
      兒玉哲也、三好哲也、小比賀聡、今西武
    • Organizer
      第6回次世代を担う有機化学シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] Design and Chemical Synthesis of a 2', 4'-BNA Probe for 7, 8-Dihydro-8-oxoguanine-containing Double Stranded DNA2008

    • Author(s)
      三好哲也(兒玉哲也)
    • Organizer
      Joint symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      京都
    • Related Report
      2008 Annual Research Report
  • [Presentation] DNA配列選択的な8-ヒドロキジグアニン検出プローブの創製研究2008

    • Author(s)
      兒玉哲也
    • Organizer
      第6回次世代を担う有機化学シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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