Development of a novel quantification method for simultaneous screeningof bioactive peptides.

• Project/Area Number: 20790028
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: GOTO Takaaki Tohoku University, 大学院・薬学研究科, 講師 (40344684)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 生理活性ペプチド / 多成分スクリーニング / 抗体 / 質量分析法 / MALDI / アンジオテンシン / 安定同位元素標識 / 生理性ペプチド

Research Abstract

A novel methodology to screen various target bioactive peptides has been examined. That is based on the group-specific immunoaffinity extraction followed by direct MALDI-TOF/MS analysis. This strategy can be used for any bioactive peptides as long as the antibodies are available. This could help for the efficient diagnostic/therapeutic marker discovery in a disease state.

Research Products

• [Journal Article] Combining [13C6]-phenylisothiocyanate and the Edman degradation reaction: a possible breakthrough for absolute quantitative proteomics together with protein identification. (2010)
  • Author(s): omoyuki Oe, Masamitsu Maekawa, Ryo Satoh, Seon Hwa Lee, Takaaki Goto
  • Journal Title: Rapid Commun. Mass Spectrom. 24 Pages: 173-179
  • Peer Reviewed
• [Journal Article] Combining[13C6]-phenylisothiocyanate and the Edman degradation reaction : a possible breakthrough for absolute quantitative proteomics together with protein identification (2010)
  • Author(s): Tomoyuki Oe
  • Journal Title: Rapid Commun.Mass Spectrom. 24 Pages: 173-179
  • Peer Reviewed
• [Journal Article] A new, major C_<27> biliary bile acid in the Red-winged tinamou(Rhynchotus rufescens) : (25R)-1β, 3α, 7α-trihydroxy-5β-cholestan-27-oic acid (2009)
  • Author(s): Lee R. Hagey
  • Journal Title: Journal of Lipid Research 50 Pages: 651-657
  • Peer Reviewed
• [Journal Article] A novel 4-oxo-2(E)-nonenal-derived modification to angiotensin II: Oxidative decarboxylation of N-terminal aspartic acid. (2008)
  • Author(s): Seon Hwa Lee, Takaaki Goto, Tomoyuki Oe
  • Journal Title: Chem. Res. Toxicol. 21 Pages: 2237-2244
  • Peer Reviewed
• [Journal Article] A novel 4-oxo-2(E)-nonenal-derived modification to angiotensin II : Oxidative decarboxylation of N-terminal aspartic acid (2008)
  • Author(s): Seon Hwa Lee
  • Journal Title: Chemical Research in Toxycology 21 Pages: 2237-2244
  • Peer Reviewed
• [Presentation] Novel Strategy to Screen Various Chemical Modifications on a Target Peptide: Combination of Group-Specific Immunoaffinity Extraction and Mass Spectrometry. (2009)
  • Author(s): Takaaki Goto, Shota Kojima, Seon Hwa Lee, Tomoyuki Oe
  • Organizer: 57th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Philadelphia, PA, USA.
  • Year and Date: 2009-06-02
• [Presentation] Novel Strategy to Screen Various Chemical Modifications on a Target Peptide : Combination of Group-Specific Immunoaffinity Extraction and Mass Spectrometry (2009)
  • Author(s): Takaaki Goto
  • Organizer: 57^<th> ASMS Conference on Mass Spectrometry
  • Place of Presentation: USA, Philadelphia, Pennsylvania
  • Year and Date: 2009-06-02
• [Presentation] 大江知行生体試料中化学修飾ペプチドの分別分析を目的とした群特異的イムノアフィニティー抽出法 (2009)
  • Author(s): 小島昌太、後藤貴章、李宣和
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] 生体試料中化学修飾ペプチドの分別分析を目的とした群特異的イムノアフィニティー抽出法 (2009)
  • Author(s): 小島昌太
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26